Biology: Cell Structure and Function Quiz
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Questions and Answers

What is a key property common to all cells?

  • Existence of a nucleus
  • Ability to move independently
  • Presence of a cell membrane (correct)
  • Ability to produce energy through photosynthesis

How does the surface-to-volume ratio affect cell size?

  • Larger cells can more efficiently perform metabolic functions
  • Smaller cells have a lower surface-to-volume ratio
  • Larger cells have a higher surface-to-volume ratio
  • Increased surface area can facilitate nutrient absorption (correct)

Which of the following correctly distinguishes prokaryotic from eukaryotic cells?

  • Prokaryotic cells have a defined nucleus; eukaryotic cells do not.
  • Prokaryotic cells possess membrane-bound organelles; eukaryotic do not.
  • Eukaryotic cells lack ribosomes; prokaryotic cells contain them.
  • Eukaryotic cells are typically larger than prokaryotic cells. (correct)

Which evidence supports the endosymbiotic theory of eukaryotic organelles?

<p>Mitochondria and chloroplasts possess their own DNA. (D)</p> Signup and view all the answers

What features must have been present in the earliest cells?

<p>Simple genetic material capable of replication (D)</p> Signup and view all the answers

What is the primary function of mitochondria in eukaryotic cells?

<p>Generate ATP using oxygen and glucose (A)</p> Signup and view all the answers

Which statement about the cytoskeleton is true?

<p>It supports the shape of the cell and keeps organelles in fixed locations. (C)</p> Signup and view all the answers

How are mitochondria inherited?

<p>From the mother only (C)</p> Signup and view all the answers

What are intermediate filaments primarily known for?

<p>Providing structural stability and durability to cells (B)</p> Signup and view all the answers

Which type of structure is responsible for cellular propulsion, similar to that of a spaceship?

<p>Cilia and Flagella (D)</p> Signup and view all the answers

What role do lysosomes play in cellular maintenance?

<p>They digest and remove old structures and organelles. (B)</p> Signup and view all the answers

What is a primary by-product of the activities of peroxisomes?

<p>Hydrogen peroxide (C)</p> Signup and view all the answers

Which structure is responsible for ATP production in the cell?

<p>Mitochondria (D)</p> Signup and view all the answers

What feature distinguishes peroxisomes from lysosomes?

<p>Peroxisomes oxidize fatty acids while lysosomes digest cellular debris. (A)</p> Signup and view all the answers

What is the significance of the low pH inside lysosomes?

<p>It activates the digestive enzymes. (B)</p> Signup and view all the answers

What is the primary function of cilia and flagella in eukaryotic cells?

<p>Motion (C)</p> Signup and view all the answers

Which structure is primarily associated with the movement of chromosomes during cell division?

<p>Centrosome (B)</p> Signup and view all the answers

What is the arrangement of microtubules in cilia and flagella referred to as?

<p>9 + 2 structure (B)</p> Signup and view all the answers

What type of organelle contains chlorophyll and is involved in photosynthesis?

<p>Chloroplasts (C)</p> Signup and view all the answers

Which of the following is NOT a function of the vacuole in plant cells?

<p>Regulating cell division (C)</p> Signup and view all the answers

What protein is responsible for the movement of microtubules in cilia and flagella?

<p>Dynein (C)</p> Signup and view all the answers

How do flagella move in eukaryotic cells?

<p>By undulating (D)</p> Signup and view all the answers

Which of the following structures is unique to plant cells?

<p>Water vacuole (B)</p> Signup and view all the answers

What is the primary role of the Golgi Complex in a cell?

<p>Collection, packaging, and distribution of proteins and lipids (A)</p> Signup and view all the answers

Which structure serves as the 'recycling truck' of the cell?

<p>Lysosome (C)</p> Signup and view all the answers

What distinguishes eukaryotic cells from prokaryotic cells?

<p>Presence of the endomembrane system (B)</p> Signup and view all the answers

What are the two types of transport associated with vesicles?

<p>Endocytosis and exocytosis (A)</p> Signup and view all the answers

What is the structure of the Golgi Complex?

<p>Flat membranes with bulging edges (B)</p> Signup and view all the answers

What is the primary product of nitrogen fixation that makes nitrogen bioavailable to plants?

<p>Ammonia (NH3) (B)</p> Signup and view all the answers

Where is the cis face of the Golgi Complex located?

<p>Near the Rough Endoplasmic Reticulum (B)</p> Signup and view all the answers

Which characteristic is NOT associated with mitochondria according to the endosymbiosis theory?

<p>Ability to photosynthesize (A)</p> Signup and view all the answers

Glycoproteins are formed by the addition of which molecule?

<p>A polysaccharide (D)</p> Signup and view all the answers

What type of vesicles would be used for exporting materials out of the cell?

<p>Exocytic vesicles (C)</p> Signup and view all the answers

How does RNA serve a crucial role in early life forms?

<p>It can catalyze chemical reactions. (A)</p> Signup and view all the answers

What role do vesicles play in intracellular transport?

<p>Transporting materials within the cell (B)</p> Signup and view all the answers

What is NOT a characteristic of prokaryotic cells?

<p>Presence of a nucleus (D)</p> Signup and view all the answers

Which statement accurately describes ribozymes?

<p>They are RNA molecules that act as enzymes. (A)</p> Signup and view all the answers

How many Golgi Complexes might a protist typically have?

<p>One to a few (B)</p> Signup and view all the answers

What key role do cell membranes play in cellular environments?

<p>They separate the internal and external environment of the cell. (C)</p> Signup and view all the answers

What evidence supports the endosymbiosis theory for the origin of mitochondria?

<p>Mitochondria can replicate independently. (D)</p> Signup and view all the answers

Which of the following processes includes the conversion of nitrogen gas (N2) into a usable form for living organisms?

<p>Nitrogen fixation (C)</p> Signup and view all the answers

Why is DNA loosely compacted during interphase?

<p>To make it readable by the cell (B)</p> Signup and view all the answers

What role do histones play in the structure of nucleosomes?

<p>They help in the condensation of chromosomes (B)</p> Signup and view all the answers

What is the advantage of chromatin being maximally compacted during cell division?

<p>It enables efficient partitioning to daughter cells (A)</p> Signup and view all the answers

What does the term TADs refer to in the context of chromatin organization?

<p>Topologically associated domains (D)</p> Signup and view all the answers

How are nucleosomes structured?

<p>DNA duplex coiled around histone proteins (B)</p> Signup and view all the answers

During which phase of the cell cycle are chromosomes most condensed?

<p>M phase (B)</p> Signup and view all the answers

What is the structural unit of DNA packaging called?

<p>Nucleosome (C)</p> Signup and view all the answers

What occurs to homologous chromosomes prior to DNA replication?

<p>They consist of one DNA molecule each (B)</p> Signup and view all the answers

What is the purpose of cohesin proteins during DNA replication?

<p>They bind sister chromatids together. (D)</p> Signup and view all the answers

During which phase does the cell synthesize a replica of its genome?

<p>S Phase (A)</p> Signup and view all the answers

What is primarily occurring during the G1 phase of interphase?

<p>Cell growth and organelle duplication occur. (C)</p> Signup and view all the answers

What is the outcome at the end of the somatic cell division process?

<p>Two identical daughter cells are formed. (B)</p> Signup and view all the answers

What distinguishes the mitotic phase from interphase?

<p>Sister chromatids are separated during the mitotic phase. (D)</p> Signup and view all the answers

In what state is DNA during interphase?

<p>Loosely compacted and not clearly defined. (B)</p> Signup and view all the answers

Which statement is true regarding the centromere?

<p>It connects sister chromatids during cell division. (D)</p> Signup and view all the answers

Which phase is primarily characterized as the longest stage of the cell cycle?

<p>G1 Phase (B)</p> Signup and view all the answers

What is the main method of bacterial cell division?

<p>Binary fission (B)</p> Signup and view all the answers

What happens during the replication of a bacterial chromosome?

<p>It is replicated in two directions (B)</p> Signup and view all the answers

Which of the following correctly describes the outcome of binary fission in bacteria?

<p>A clone of the original cell is produced (B)</p> Signup and view all the answers

How is bacterial DNA compacted within the cell?

<p>Through a process called supercoiling (A)</p> Signup and view all the answers

What structure forms to separate the two new cells during bacterial division?

<p>Septum (D)</p> Signup and view all the answers

What is the location of bacterial DNA within a cell known as?

<p>Nucleoid region (A)</p> Signup and view all the answers

Which phase of the eukaryotic cell cycle is primarily responsible for DNA replication?

<p>S phase (D)</p> Signup and view all the answers

In contrast to eukaryotic cell division, what is a key characteristic of bacterial cell division?

<p>Clonal reproduction (D)</p> Signup and view all the answers

What is the term for a cell with two sets of homologous chromosomes?

<p>Diploid (A)</p> Signup and view all the answers

Which type of cell division do somatic cells undergo?

<p>Mitosis (A)</p> Signup and view all the answers

Which of the following correctly defines germ-line cells?

<p>Diploid cells that undergo meiosis to produce haploid gametes (B)</p> Signup and view all the answers

How many chromosomes do humans have in diploid somatic cells?

<p>46 (A)</p> Signup and view all the answers

What does the term 'ploidy' refer to in genetics?

<p>The number of sets of homologous chromosomes (A)</p> Signup and view all the answers

Which of the following statements is true about haploid cells?

<p>They have one set of chromosomes. (B)</p> Signup and view all the answers

In what way do offspring inherit genetic material from their parents?

<p>From a combination of both parents' chromosomes (B)</p> Signup and view all the answers

Which feature distinguishes somatic cells from germ-line cells?

<p>Somatic cells undergo mitosis, while germ-line cells undergo meiosis (A)</p> Signup and view all the answers

What initiates the anaphase during somatic cell division?

<p>Centromeres splitting due to cohesion protein removal. (A)</p> Signup and view all the answers

Which of the following occurs during telophase?

<p>Two daughter nuclei form. (B)</p> Signup and view all the answers

Which phase of mitosis is characterized by the alignment of chromosomes on the metaphase plate?

<p>Metaphase (B)</p> Signup and view all the answers

What is the role of the cleavage furrow during cytokinesis in animal cells?

<p>Pinching the cell into two identical daughter cells. (A)</p> Signup and view all the answers

What occurs during the G1 phase of the cell cycle?

<p>Cells may enter a resting state called G0 (D)</p> Signup and view all the answers

How do plant cells differ from animal cells during cytokinesis?

<p>Plant cells assemble a membrane using vesicles. (A)</p> Signup and view all the answers

What happens to the chromosomes at the end of anaphase?

<p>They migrate to opposite poles. (C)</p> Signup and view all the answers

Which of the following describes a characteristic of prophase in mitosis?

<p>Nuclear envelope disassembly begins (B)</p> Signup and view all the answers

Which structure is responsible for the movement of chromosomes during cell division?

<p>Kinetochore microtubule (C)</p> Signup and view all the answers

What happens during cytokinesis?

<p>Cytoplasm is divided into two daughter cells (B)</p> Signup and view all the answers

What feature characterizes the end of metaphase?

<p>Chromosomes line up at the metaphase plate. (D)</p> Signup and view all the answers

Which phase of the cell cycle typically sees the most variation in length between different cell types?

<p>G1 phase (D)</p> Signup and view all the answers

During which phase do microtubules attach to the centromeres of chromosomes?

<p>Prometaphase (B)</p> Signup and view all the answers

Which statement is true about the frequency of cell division?

<p>Neurons do not appear to divide after maturity (D)</p> Signup and view all the answers

What is indicated when a cell is in the G0 phase?

<p>It may resume division after a long pause (B)</p> Signup and view all the answers

Which role do centrioles play during mitosis?

<p>They form the spindle apparatus (D)</p> Signup and view all the answers

What is the first step in Mendel's experimental method?

<p>Produce true-breeding strains for each trait (B)</p> Signup and view all the answers

Which of the following traits did Mendel study in his experiments?

<p>Seed shape (C)</p> Signup and view all the answers

What is the result of crossing a true-breeding purple flower with a true-breeding white flower?

<p>Offspring will have only purple flowers (B)</p> Signup and view all the answers

What process did Mendel use to prevent self-fertilization in his pea plants?

<p>Removing the anthers from flowers (A)</p> Signup and view all the answers

How many different traits did Mendel produce true-breeding strains for?

<p>7 (A)</p> Signup and view all the answers

Which of these is NOT a method included in Mendel's experimental method?

<p>Alter the genetic material artificially (C)</p> Signup and view all the answers

What is the purpose of counting the number of offspring in Mendel's experiments?

<p>To calculate the inheritance pattern of traits (C)</p> Signup and view all the answers

What do true-breeding strains guarantee in Mendel's experiments?

<p>Uniformity in trait expression over generations (D)</p> Signup and view all the answers

What is a key characteristic of a test cross?

<p>It involves crossing a dominant phenotype with a homozygous recessive. (A)</p> Signup and view all the answers

In a monohybrid cross, what is being studied?

<p>Variations of a single trait. (C)</p> Signup and view all the answers

During a dihybrid cross, what is the key purpose?

<p>To observe the inheritance of two separate traits. (B)</p> Signup and view all the answers

What is the expected phenotype of the F1 generation in a dihybrid cross?

<p>Only dominant phenotypes for each trait. (A)</p> Signup and view all the answers

Which genotype represents a heterozygous individual?

<p>Pp (A)</p> Signup and view all the answers

How many gametes can a dihybrid plant with the genotype RrYy produce?

<p>4 (A)</p> Signup and view all the answers

What is the term used to describe true breeding parental generations in genetic crosses?

<p>P generation (B)</p> Signup and view all the answers

In a Punnett square, what do the letters represent?

<p>The alleles of the offspring. (D)</p> Signup and view all the answers

What is the genotype of the F1 generation offspring when crossing a true-breeding purple parent with a true-breeding white parent?

<p>Pp (A)</p> Signup and view all the answers

In a Punnett square, which type of allele expresses itself over the other?

<p>Dominant allele (B)</p> Signup and view all the answers

What phenotypes are possible in the F2 generation resulting from a cross of F1 heterozygous purple offspring?

<p>Purple and white (C)</p> Signup and view all the answers

What percentage of F2 offspring from a Pp x Pp cross are expected to be homozygous recessive (pp)?

<p>25% (D)</p> Signup and view all the answers

What type of gametes do true-breeding parents produce?

<p>One type of gamete (A)</p> Signup and view all the answers

Which phenotype corresponds with the homozygous dominant genotype?

<p>Homozygous purple flowers (C)</p> Signup and view all the answers

Why are all F1 offspring purple when crossing a purple and a white parent?

<p>The dominant trait completely masks the recessive trait (D)</p> Signup and view all the answers

What is the expected ratio of phenotypes in the F2 generation from a dihybrid cross?

<p>3:1 (D)</p> Signup and view all the answers

What ratio of dominant to recessive traits is observed in the F2 generation from self-fertilized hybrid offspring?

<p>3:1 (C)</p> Signup and view all the answers

What ratio represents the typical genetic makeup of the F3 generation?

<p>1:2:1 (C)</p> Signup and view all the answers

What is the definition of a dominant allele?

<p>An allele that is always expressed in the phenotype (A)</p> Signup and view all the answers

What does it mean when an organism is described as homozygous?

<p>Having two identical alleles for a specific gene (D)</p> Signup and view all the answers

Which statement about alleles is true?

<p>Alleles remain discrete and do not blend with one another (A)</p> Signup and view all the answers

What does the term phenotype refer to?

<p>The physical appearance of an organism (A)</p> Signup and view all the answers

In Mendel's experiments, how were alternative traits expressed in the progeny of a particular cross?

<p>In the ratio of 3:1 (B)</p> Signup and view all the answers

Which of the following statements accurately summarizes Mendel's five-element model of inheritance?

<p>Alleles are inherited in pairs, but not as blends (A)</p> Signup and view all the answers

What color flowers are produced in the first filial generation (F1) when a true-breeding purple flower is crossed with a true-breeding white flower?

<p>Only purple flowers (A)</p> Signup and view all the answers

What does the term 'dominant trait' refer to in the context of genetics?

<p>A trait that is always expressed in hybrid offspring (C)</p> Signup and view all the answers

What phenotypic ratio is expected in the F2 generation after self-fertilizing hybrid F1 plants?

<p>3:1 ratio of purple to white (A)</p> Signup and view all the answers

What is observed when conducting a reciprocal cross between two true-breeding plants?

<p>Same offspring phenotype regardless of the cross direction (B)</p> Signup and view all the answers

Which statement accurately differentiates hybrid offspring from their true-breeding parents?

<p>Hybrids display one parent's trait exclusively (D)</p> Signup and view all the answers

How is the recessive trait treated in the F1 generation when true-breeding strains are crossed?

<p>Completely masked and not expressed (B)</p> Signup and view all the answers

What is the role of self-fertilization in the F1 generation for producing the F2 generation?

<p>To stabilize the traits of the F1 hybrid (C)</p> Signup and view all the answers

What phenomenon is completely absent in the offspring of the initial cross between two true-breeding plants?

<p>Identification of intermediate characteristics (B)</p> Signup and view all the answers

What is the primary purpose of a test cross in genetics?

<p>To determine the unknown genotype of an individual (A)</p> Signup and view all the answers

What does a purple-flowered pea plant's phenotype indicate?

<p>The plant may be either homozygous or heterozygous. (A)</p> Signup and view all the answers

In a test cross, what genotype is the unknown genotype crossed with?

<p>Homozygous recessive (A)</p> Signup and view all the answers

Which Punnett square outcome would indicate that a purple-flowered plant is likely homozygous?

<p>100% purple flowers (A)</p> Signup and view all the answers

What genetic feature allows a plant with the purple phenotype to potentially be heterozygous?

<p>The dominance of the purple allele (D)</p> Signup and view all the answers

What conclusion can be made if a test cross results in offspring with a mix of purple and white flowers?

<p>The unknown plant is heterozygous. (C)</p> Signup and view all the answers

In the context of Mendelian genetics, how is the term 'homozygous' best defined?

<p>Having identical alleles for a trait (B)</p> Signup and view all the answers

What type of genetic cross demonstrates the principle of segregation in inheritance?

<p>Monohybrid cross (C)</p> Signup and view all the answers

Flashcards

Cell Theory

A fundamental principle in biology stating that all living organisms are composed of cells and that all cells arise from pre-existing cells.

Surface-to-Volume Ratio

The ratio of a cell's outer surface area to its volume. It affects the rate of diffusion and the efficiency of nutrient uptake and waste removal.

Prokaryotic Cells

Simple cells lacking a nucleus and other membrane-bound organelles, usually found in bacteria and archaea.

Eukaryotic Cells

Complex cells with a nucleus and other membrane-bound organelles, found in plants, animals, fungi, and protists.

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Endosymbiotic Theory

The theory explaining the origin of eukaryotic organelles like mitochondria and chloroplasts, which were once free-living prokaryotes that were engulfed by larger cells.

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Lysosomes

Organelles containing enzymes that break down proteins, nucleic acids, lipids, and carbohydrates. They also digest and remove old cell structures. The low pH inside activates the enzymes.

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Peroxisomes

Small organelles similar to lysosomes, but specialize in oxidizing fatty acids and amino acids. They produce hydrogen peroxide as a byproduct, which is broken down by catalase enzyme.

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What is the function of catalase in peroxisomes?

Catalase is an enzyme that breaks down hydrogen peroxide (H2O2) into water and oxygen, preventing its accumulation and damaging effects on the cell.

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Mitochondria

Rod-shaped organelles with two membranes: an outer membrane and an inner membrane folded into cristae. They are the 'powerhouses' of the cell, producing ATP (energy) during cellular respiration.

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What is the function of the cristae in mitochondria?

Cristae are the folds of the inner membrane in mitochondria. They increase the surface area for chemical reactions involved in ATP production.

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Endomembrane System

A network of membranes within the cytoplasm that divides the cell into compartments for different functions.

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Golgi Complex

A series of flattened sacs within the cytoplasm of eukaryotic cells that process, modify, and package proteins and lipids.

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Cis Face

The receiving side of the Golgi Complex, located close to the Endoplasmic Reticulum (ER).

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Trans Face

The exit side of the Golgi Complex, where processed proteins and lipids are packaged into vesicles for transport.

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Vesicle

A small membrane-bound sac that transports materials within and outside the cell.

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Endocytosis

The process of bringing material INTO the cell.

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Exocytosis

The process of exporting material OUT of the cell.

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Glycoprotein vs Glycolipid

Glycoproteins have a sugar molecule attached to a protein, while glycolipids have a sugar molecule attached to a lipid.

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Mitochondria: Powerhouse of the Cell

Mitochondria are the powerhouses of the cell, responsible for generating ATP, the cell's energy currency. They utilize oxygen and glucose to produce energy, releasing carbon dioxide and water as byproducts.

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Mitochondrial DNA

Mitochondria have their own circular DNA, separate from the cell's nuclear DNA. However, they still rely on genes from the nucleus to function properly.

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What is the cytoskeleton's function?

The cytoskeleton is a network of protein fibers found in all eukaryotic cells. It provides structural support, giving the cell its shape. It also helps keep organelles in their designated locations, similar to a cell's internal scaffolding.

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Actin Filaments: Building Blocks for Movement

Actin filaments, also known as microfilaments, are part of the cytoskeleton. They are involved in muscle cell contraction, allowing for movement.

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Intermediate Filaments: Durable and Diverse

Intermediate filaments are the most durable part of the cytoskeleton. They're made of different proteins and provide structural support to cells. Keratin, a key component of hair and nails, is an example of an intermediate filament protein.

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Nitrogen Fixation

The process where nitrogen-fixing bacteria convert atmospheric nitrogen gas (N2) into a usable form (ammonia, NH3) for plants.

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RNA World Hypothesis

The theory that RNA was the primary form of genetic material in early life, capable of both storing genetic information and catalyzing chemical reactions.

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Plasma Membrane

The outer boundary of a cell, separating its internal environment from the external environment, regulating what enters and exits the cell.

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Mitochondria: Origin

Mitochondria originated from an ancient bacterial endosymbiosis event, where a prokaryote was engulfed by a eukaryotic cell.

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Chloroplast: Origin

Chloroplasts originated from an ancient endosymbiotic event, where a photosynthetic cyanobacterium was engulfed by a eukaryotic cell.

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Prokaryote Characteristics

Prokaryotes are the simplest organisms, lacking a nucleus, having free circular DNA, and relying on a rigid cell wall for structure.

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Ribozyme

An RNA molecule that acts as an enzyme, catalyzing specific biochemical reactions.

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Cilia

Short, hair-like structures that help in cell movement. They are abundant in number and found in eukaryotic cells.

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Flagellum

A long, whip-like structure used for cell movement. They are usually found in smaller numbers than cilia.

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What makes cilia and flagella move?

The movement of cilia and flagella is powered by microtubules arranged in a 9+2 structure. Dynein, a motor protein, helps these microtubule pairs move past each other.

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Centriole

A small, cylindrical structure found in eukaryotic cells. They are involved in cell division by moving chromosomes to the poles of the cell.

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What is the role of the centrosome?

The centrosome is a region within a cell that contains two centrioles arranged perpendicular to each other. It is involved in organizing microtubules and plays a crucial role in cell division.

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Central Vacuole

Large, fluid-filled sacs found primarily in plant cells. They play a role in storage, water balance and maintaining cell shape.

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Chloroplast

Organelles found in plants and algae. They contain chlorophyll, which is used for photosynthesis, the process of converting light energy into chemical energy.

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What makes chloroplasts special?

Chloroplasts are unique because they have their own DNA and ribosomes, indicating their possible origin as ancient bacteria.

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Binary Fission

Asexual reproduction in bacteria where a single cell divides into two identical daughter cells.

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Origin of Replication

The specific point on a bacterial chromosome where DNA replication begins.

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Septum

A new cell wall that forms during bacterial division, separating the two daughter cells.

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Loop Domain

A compact structure formed by DNA binding proteins, helping to condense the bacterial chromosome.

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Supercoiling

The process of further compacting DNA by twisting it upon itself, allowing for efficient packaging inside the bacterial cell.

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Nucleoid Region

The area in the cytoplasm of a bacterial cell where the DNA is located.

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Mitosis vs. Binary Fission

Mitosis is cell division in eukaryotes with complex, linear chromosomes and multiple phases. Binary fission is cell division in prokaryotes with a single, circular chromosome and simpler steps.

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Clonal Reproduction

A form of reproduction where offspring are genetically identical to the parent organism.

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Homologous Chromosomes

Pairs of chromosomes with the same genes. Each chromosome in the pair comes from a different parent.

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Ploidy

The number of sets of homologous chromosomes in a cell.

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Haploid (n)

A cell with only one set of chromosomes. For example, a sperm or an egg cell.

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Diploid (2n)

A cell with two sets of chromosomes. Most human cells are diploid.

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Somatic Cell

Any cell in a multicellular organism that is not a sperm or egg cell. These cells divide by mitosis.

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Germ-line Cell

A type of diploid cell that can undergo meiosis to produce haploid gametes.

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Mitosis

A type of cell division that produces two identical daughter cells. Used by somatic cells to grow and repair tissues.

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Meiosis

A type of cell division that produces four haploid gametes (sperm or egg cells) from a diploid cell. Used for sexual reproduction.

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Somatic Cell Division

The process of cell division that produces two identical daughter cells, each with the same number of chromosomes as the parent cell.

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Sister Chromatids

Identical copies of a single replicated chromosome, attached at the centromere.

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Centromere

The constricted region of a chromosome where sister chromatids are attached.

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Interphase

The phase of the cell cycle where the cell grows, copies its DNA, and prepares for cell division.

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G1 Phase

The first growth phase of interphase, where the cell grows and produces proteins.

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S Phase

The phase of interphase where DNA is replicated.

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G2 Phase

The second growth phase of interphase, where the cell prepares for mitosis.

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Chromatin

A complex of DNA and proteins that make up chromosomes. It's found in the nucleus of eukaryotic cells.

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Nucleosome

The basic unit of DNA packaging in eukaryotes. It consists of a segment of DNA wrapped around eight histone proteins.

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Histones

Proteins that DNA wraps around to form nucleosomes. They have a positive charge that attracts the negatively charged DNA.

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TADs (Topologically Associated Domains)

Regions of a chromosome that are physically linked and interact more frequently than other parts of the chromosome.

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Why does DNA condense during cell division?

DNA condenses into chromosomes during cell division to ensure that each daughter cell receives a complete copy of the genetic material. It also makes it easier to move the chromosomes around the cell.

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Radial Looping

A process that helps compact DNA during cell division. Loops of DNA are formed and anchored to a protein scaffold.

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Condensin Proteins

Proteins that help with the folding and compaction of DNA during cell division.

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What is interphase?

The longest phase of the cell cycle, where the cell grows, duplicates its organelles, and copies its DNA.

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Cytokinesis

The division of the cytoplasm, which follows mitosis and results in two separate daughter cells.

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Chromosomes condense

During prophase, chromosomes condense into visible structures, each consisting of two identical sister chromatids.

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What is the purpose of Prophase?

Prophase is the first stage of mitosis where the chromatin condenses into visible chromosomes. This prepares the genetic material for division.

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What happens during Prometaphase?

In Prometaphase, the nuclear envelope breaks down, allowing microtubules from the spindle apparatus to attach to chromosomes at the centromere.

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What's the Metaphase plate?

The metaphase plate is the imaginary plane where chromosomes align during Metaphase, equidistant from the spindle poles.

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Explain Anaphase.

In Anaphase, sister chromatids separate and move towards opposite poles of the cell, driven by the shortening of kinetochore microtubules.

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What is Telophase?

Telophase is the final stage of mitosis, where chromosomes reach the poles and decondense. The nuclear envelope reforms, and the spindle apparatus disassembles.

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What is Cytokinesis?

Cytokinesis is the division of the cytoplasm, physically separating the two daughter cells.

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Difference between plant and animal cytokinesis?

In animal cells, cytokinesis involves formation of a cleavage furrow. In plant cells, a cell plate is formed by vesicles fusing together.

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What is the role of actin filaments in animal cytokinesis?

Actin filaments play a key role in animal cytokinesis, forming a contractile ring that pinches the cell membrane inward, dividing the cytoplasm.

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True-Breeding Strain

A type of organism that consistently produces offspring with the same traits when self-fertilized.

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F1 Generation

The first generation of offspring produced by crossing two true-breeding parents.

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Dominant Trait

A trait that appears in the F1 generation when two contrasting true-breeding parents are crossed.

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Recessive Trait

A trait that is masked in the F1 generation but reappears in the F2 generation.

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F2 Generation

The second generation of offspring produced by self-fertilizing the F1 generation.

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Phenotypic Ratio

The observed ratio of different phenotypes (physical traits) in a population.

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3:1 Phenotypic Ratio

The characteristic ratio of phenotypes observed in the F2 generation when crossing two true-breeding parents with contrasting traits.

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Reciprocal Cross

A cross where the parental phenotypes are reversed to confirm if a trait is sex-linked.

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Mendel's experimental method

A three-step process involving producing true-breeding strains, cross-fertilizing them, and observing offspring over generations.

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Cross-fertilization

The process of transferring pollen from the anthers of one plant to the stigma of another plant.

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Why remove anthers?

Mendel removed anthers to prevent self-fertilization and ensure only the desired cross-fertilization occurred.

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Parental generation (P)

The original generation used in a cross-fertilization experiment, representing the starting point for studying inheritance.

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First filial generation (F1)

The offspring of a cross-fertilization between two true-breeding strains.

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Second filial generation (F2)

The offspring produced by self-fertilizing the F1 generation, revealing the inheritance patterns of the traits.

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What is the importance of Mendel's experiments?

Mendel's experiments laid the foundation for understanding the principles of inheritance, demonstrating how traits are passed from one generation to the next.

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Mendel's 3:1 Ratio

When self-fertilizing hybrid offspring for several generations, the F2 generation consistently shows a 3:1 ratio of dominant to recessive traits.

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No Blending

Mendel observed that traits did not blend in offspring, meaning there was no intermediate expression (like pale purple flowers). Instead, one trait was dominant, the other recessive.

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Allele

An alternative form of a gene. For example, a flower could have the purple allele or the white allele.

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Homozygous vs. Heterozygous

Homozygous: Two identical alleles for a trait (e.g., purple-purple). Heterozygous: Two different alleles for a trait (e.g., purple-white).

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Dominant vs. Recessive

Dominant: The allele that is always expressed (denoted by a capital letter). Recessive: The allele that is only expressed if two copies are present (denoted by a lowercase letter).

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Phenotype

The physical appearance of a trait. For example, a purple flower or a white flower.

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Mendel's Five-Element Model

A summary of Mendel's key findings about inheritance: parents transmit discrete factors (genes), individuals receive one copy from each parent, not all copies are identical (alleles), alleles remain discrete, and dominant allele expression masks recessive alleles.

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Punnett Square

A diagram used to predict the possible genotypes and phenotypes of offspring from a cross between two parents. It shows the combination of alleles from each parent.

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Heterozygous

Having two different alleles for a particular trait. For example, Pp for a flower where 'P' is purple and 'p' is white.

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Homozygous

Having two identical alleles for a particular trait. For example, PP or pp for a flower.

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Test Cross

Crossing an individual with an unknown genotype with a homozygous recessive individual to determine the unknown genotype.

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Monohybrid Cross

A cross between two individuals that differ in a single trait. Example: flower color, where one parent is red and the other is white.

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Dihybrid Cross

A cross between two individuals that differ in two traits. Example: seed color and seed shape.

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True Breeding

An organism that always produces offspring with the same phenotype as itself. It is homozygous for the trait considered.

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Dominant Phenotype

The phenotype that is expressed in a heterozygous individual. The allele responsible for it masks the recessive allele.

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Dominant Allele

An allele that masks the expression of another allele.

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Recessive Allele

An allele whose expression is masked by a dominant allele.

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Study Notes

General Biology 1 - Fall 2024

  • Course focusing on the study of cells, as part 2.
  • Textbook used: Raven, Biology, 13th edition.
  • Key topics covered by these notes include Cell Structure, Chemical Basis of Life, Organelles, Endomembrane System, the Golgi Complex, Vesicles, Lysosomes and Peroxisomes, Mitochondria, Cytoskeleton, Cilia and Flagella, Centrosomes, Plant cells and Vacuoles, Chloroplasts, how life began on Earth, prokaryotes and eukaryotes (differences).

Chemical Basis of Life

  • Cell Theory Summary: Summarize the Cell Theory and list the key properties common to all cells.
  • Surface Area to Volume Ratio: Discuss the impact of surface-to-volume ratios on cell size, and biological processes in general.
  • Prokaryotic vs. Eukaryotic Cells: Distinguish between prokaryotic and eukaryotic cells.
  • Cellular Structures: Identify and/or explain the function of various cellular structures (organelles) found in typical eukaryotic cells.
  • Cell Comparison: Compare the structural aspects of cells found in bacteria, protists, plants, animals, and fungi.
  • Origins of Life: Briefly discuss the possible origins of life, and the properties of life.
  • Early Cells: Describe features that must have been present in the earliest cells.
  • Endosymbiotic Theory: Describe the endosymbiotic theory regarding eukaryotic organelles, including evidence supporting the theory.

Organelles

  • The cell is like a medieval city.
  • Organelles are like all the buildings of a city.
  • The endomembrane system is a series of membranes within the cytoplasm of a eukaryotic cell.

Endomembrane System

  • The endomembrane system is a series of membranes throughout the cytoplasm (surrounds the organelles).
  • It divides the cell into compartments where different cellular functions occur.
  • The presence of endomembrane is a fundamental distinction between eukaryotes and prokaryotes.
    • Components include control center (nucleus), assembly line (endoplasmic reticulum), workbenches (ribosomes), distribution center (Golgi complex), and cleaning crew (lysosomes).

Golgi Complex

  • Function: Collection, packaging, and distribution of proteins and lipids; modification of proteins and lipids.
  • Number: Protists: 1 to a few, Animals: >20, Plants: >100.
  • Structure: Flattened membranes with bulging edges. Two key faces: cis (receiving) and trans (exit) faces.

Vesicles

  • Structure: Small membrane sacs made of a phospholipid bilayer.
  • Function: Transporting materials within the cell. Transport material "in and out" of the cell.
  • Types of Transport: Endocytosis (import into cell) and exocytosis (export from cell).

Cell structure: Lysosomes and Peroxisomes

  • Lysosomes: Small round vesicles that arise from the Golgi apparatus, functioning as the cell's recycling centers. Contain enzymes that break down waste, old structures, and organelles. Key to having a low internal pH to activate digestive enzymes.
  • Peroxisomes: Similar to lysosomes but smaller; involved in oxidizing fatty acids and some amino acids generating hydrogen peroxide; contain catalase to break down hydrogen peroxide.

Cell structure: Mitochondria

  • Structure: Rod-shaped organelles with two membranes (outer and inner); inner membrane folds into cristae; matrix (innermost part) contains DNA and ribosomes.
  • Function: Powerhouses of the cell, generating ATP (energy currency); use oxygen and glucose for energy production, producing CO2 and water.
  • Fun Facts: Mitochondria have their own DNA (mtDNA), can divide independently, and are inherited only from the mother.

Cytoskeleton

  • Structure: Network of protein fibers found in all eukaryotic cells.
  • Function: Supports cell shape; keeps organelles in fixed locations.
  • Types: Actin filaments (also called microfilaments), Microtubules, Intermediate filaments.

Cilia and Flagella

  • Function: Both are used for cell motion; cilia are more numerous and shorter; flagella more lengthy and are few, if any in a single cell.
  • Examples: Sperm tails (flagella). Cilia can be found in the respiratory tract or uterine tubes to move substances.
  • Structure: Nine pairs of microtubules surrounding two central microtubules, a 9 + 2 arrangement. Dynein proteins cause microtubules to move.

Centrosome

  • Structure: Pair of centrioles arranged perpendicularly to each other.
  • Function: Involved in cell division, specifically movement of chromosomes to cell ends during cell division.

Plant Cells

  • Special Structures: Central vacuole, Cell wall (peptidoglycan), Chloroplasts (photosynthesis), No centrioles.
  • Vacuole: Function in storage of sugars, salts, and pigments; waste disposal; and maintenance of water balance. The vacuolar membrane is called the tonoplast and contains channels for water.
  • Chloroplast: Two membranes surrounding the organelle; contain chlorophylls for photosynthesis; have their own DNA and ribosomes; involved in photosynthesis, converting light energy to chemical energy.

How Life Began on Earth

  • Metabolic Evolution Landmarks: Oxygenic photosynthesis; carbon fixation (12C isotope).
  • Nitrogen Fixation: Nitrogen gas (N₂) is essential for life (DNA, amino acids), but unavailable to most organisms; nitrogen fixation is a vital process by microbes creating usable nitrogen for plants and other organisms to use.
  • RNA Hypothesis: Earliest life may have used RNA as the primary informational molecule, as RNA can store genetic information, act enzymatically in ribozymes, and form ribosomal structures.
  • Cell Membranes: These are crucial for separating the internal environment from the external environment, and are vital in increasing the probability of biochemical reactions.

Mitochondria Endosymbiosis Theory

  • Capture of Ancestral Bacteria: Mitochondria may have originated from aerobic bacteria that were engulfed by an ancestral eukaryotic cell.
  • Evidence: Double membrane structure (outer from host, inner from bacteria); contain their own DNA and ribosomes; can multiply independently.

Chloroplast Endosymbiosis Theory

  • Capture of Photosynthetic Bacteria (secondary): Chloroplasts may have originated from photosynthetic bacteria that were engulfed by an ancestral eukaryotic cell.
  • Evidence: Double membrane structure (inner from host, outer from bacteria); contain their own DNA and ribosomes; can multiply independently.
  • Secondary Endosymbiosis: Involves an additional engulfing event between eukaryotic cells further supporting the theory

Endosymbiosis Theory

  • Proposing that mitochondria and chloroplasts were originally bacteria that were engulfed by a larger cell.

Prokaryotes

  • Prokaryotes vs. Eukaryotes Differences: In summary, Prokaryotes lack a membrane-bound nucleus and other organelles, generally are smaller in size, and contain a single circular DNA molecule. Eukaryotes have a membrane-bound nucleus, more complex organelles, and multiple linear chromosomes.
  • Characteristics: Prokaryotes are the simplest organisms. They lack nuclei and other membrane-bound organelles.
  • Bacterial Cell Walls: The majority of bacterial cell walls incorporate peptidoglycan, a unique polymer that aids in preserving the bacterial cell's shape and resisting the uptake or loss of water. This differs from peptidoglycan which is present in plants and fungi.
  • Archaeal Cell Walls: Differing from bacteria, archaea lack peptidoglycan and their cell wall structures vary greatly. The membrane lipids are made from a different chemical structure.
  • Prokaryotic Flagella: Flagella help in cell motion. It's a rotary motor across the plasma membrane using energy from proton gradients.

Difference Between Prokaryotes and Eukaryotes

  • Eukaryotes possess: membrane-bound nucleus, complex endomembrane system and compartmentalization, and cytoskeleton.

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Test your knowledge on key properties common to all cells, the differences between prokaryotic and eukaryotic cells, and the roles of various organelles such as mitochondria and lysosomes. This quiz also covers important theories like the endosymbiotic theory and cellular maintenance mechanisms.

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