Questions and Answers
What is the primary purpose of a microbiological safety cabinet?
To protect the laboratory worker and the surrounding environment from pathogens.
A biological refrigerator is used to preserve cultures of bacteria, yeast, and other microbes at temperatures between ______ °C.
2-8
What temperature is typically set in an incubator for microorganism cultivation?
35-37
Which type of incubator is used to cultivate mammalian cells?
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A CO2 incubator is an anaerobic incubator.
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What is the standard setting for sterilization in a hot air oven?
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Hot air ovens are used for sterilizing materials such as ______ and ______.
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Study Notes
Biological Safety Cabinet
- An enclosed, ventilated workspace designed for safe handling of materials contaminated with pathogens.
- Protects laboratory workers and the environment from exposure to biohazards.
- Operates according to established biosafety levels.
Biological Refrigerator
- Preserves bacteria, yeast, and other microbes at temperatures between 2-8°C.
- Lower temperatures lead to decreased metabolic rates in living organisms.
- Distinct from regular refrigerators as it lacks a freezer compartment.
Deep Freezer
- Used for storage of plasma, blood components, vaccines, and microorganisms at temperatures below 2°C.
- Features a microcontroller-based temperature system with a digital display for monitoring set and actual temperature values.
Incubator
- Provides controlled conditions, specifically temperature settings of 35-37°C, for cultivation of microorganisms.
- Designed with a glass cover to protect cultures from microbial contamination and to prevent odors.
- Ideal for organisms like E. coli and Saccharomyces cerevisiae.
Types of Incubators
-
Carbon Dioxide Incubator:
- Cultivates mammalian cells and maintains CO2 levels of 5-10%.
- Contains typical atmospheric oxygen levels at about 20-21% with high humidity (>95%).
-
Non Carbon Dioxide Incubator:
- Operates with room air, containing normal oxygen levels without added CO2.
-
Anaerobic Incubator:
- Designed for isolating capnophiles; features a low-oxygen atmosphere.
- CO2 incubators contain some oxygen, differentiating them from true anaerobic settings.
Hot Air Oven / Oven Sterilizer
- Utilizes dry heat sterilization through oxidation for sterilizing laboratory instruments and materials.
- Originally developed by Pasteur, it uses standard settings for effective sterilization:
- 1.5 to 2 hours at 160-165°C
- 1 hour at 170-180°C
- 6 to 12 minutes for specific applications.
- Requires that items to be sterilized, such as metals and glassware, are wrapped in clean manila paper.
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Description
This quiz covers the essentials of biological safety cabinets, focusing on their design, purpose, and importance in microbiological work. Learn about the enclosed, ventilated environments necessary for safe laboratory procedures. Test your understanding of safety protocols when working with microbiological agents.
