Lab Instrument: Incubator & Safety Cabinet
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Questions and Answers

What is the primary function of a Biological Safety Cabinet?

  • To maintain temperature for laboratory samples
  • To analyze the growth of tissues
  • To protect the user, product, and environment from hazardous microorganisms (correct)
  • To provide warmth for cell cultures

Class I Biological Safety Cabinets have a front opening and an exhaust HEPA filter.

True (A)

What should be done to disinfect the work surface of a Biological Safety Cabinet?

Wipe the work surface with 70% ethanol or other appropriate disinfectant.

The continuous airflow in a Biological Safety Cabinet is released through a __________ filter.

<p>HEPA</p> Signup and view all the answers

Match the following types of Biological Safety Cabinets with their features:

<p>Class I = Front opening, HEPA filtered exhaust Class II = Front opening, supply HEPA filter, and exhaust HEPA filter Class III = Gas-tight and with HEPA filtered air Class II, Type A1 = Recirculates air with some exhausted air</p> Signup and view all the answers

Which of the following is NOT a protection mechanism of Biological Safety Cabinets?

<p>Temperature Control (C)</p> Signup and view all the answers

The Class II Biological Safety Cabinet includes a common plenum and a blower.

<p>True (A)</p> Signup and view all the answers

List one application of tissue culture techniques.

<p>Pharmaceutical and biochemical studies.</p> Signup and view all the answers

Which type of incubator is designed to work at temperatures close to or below ambient temperature?

<p>Cooled incubators (B)</p> Signup and view all the answers

Carbon dioxide incubators maintain a CO2 concentration in air ranging from 5% to 20%.

<p>True (A)</p> Signup and view all the answers

What should be done before beginning work in a Biological Safety Cabinet (BSC)?

<p>Plan the work operation in advance. (D)</p> Signup and view all the answers

It is safe to use an open flame inside a Biological Safety Cabinet.

<p>False (B)</p> Signup and view all the answers

Name one use of laboratory incubators in biological applications.

<p>To grow bacteria or cell cultures.</p> Signup and view all the answers

What disinfectant concentration is recommended for cleaning the work surface of a BSC after use?

<p>70% ethanol</p> Signup and view all the answers

A __________ incubator is used to control both temperature and humidity for biological samples.

<p>humidity</p> Signup and view all the answers

What is a main feature of standard incubators?

<p>They include an electric heater and can reach temperatures up to 100°C. (B)</p> Signup and view all the answers

After completing work in a BSC, run the cabinet for at least ______ minutes with no activity to purge airborne contaminants.

<p>3</p> Signup and view all the answers

Match the following types of incubators with their descriptions:

<p>Standard incubator = Temperature range up to 100°C Cooled incubator = Operates below ambient temperature Humidity incubator = Controls both temperature and humidity CO2 incubator = Maintains CO2 levels for biological samples</p> Signup and view all the answers

Match the following procedures with their corresponding purpose in a Biological Safety Cabinet:

<p>Keeping the BSC on = Prevents contamination spread Wiping surfaces with disinfectant = Removes potential biohazards Limiting access to one person = Protects air curtain integrity Avoiding materials on air intake grille = Maintains protective airflow</p> Signup and view all the answers

Which of the following is NOT recommended in a Biological Safety Cabinet?

<p>Placing equipment on the air intake grille (B)</p> Signup and view all the answers

Which part of an incubator is responsible for circulating air?

<p>Air circulating fan</p> Signup and view all the answers

Two people can safely work in a BSC at the same time without impacting the safety measures.

<p>False (B)</p> Signup and view all the answers

Biological safety cabinets are classified based on their ability to maintain a sterile environment.

<p>True (A)</p> Signup and view all the answers

What is the primary purpose of running the Biological Safety Cabinet for 5-10 minutes before starting work?

<p>To clean itself</p> Signup and view all the answers

Flashcards

Cell and tissue cultures

Growing animal or plant tissue fragments in controlled environments for analysis.

Biological Safety Cabinet (BSC)

A ventilated enclosure that protects users, products, and the environment from harmful aerosols during experiments.

Class 1 BSC

A BSC with an open front, HEPA filtered exhaust, and no internal air recirculation.

Class 2 BSC

A BSC with a front opening, HEPA filtered exhaust and supply air. It includes a plenum delivering HEPA-filtered air.

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Class 3 BSC

A BSC that is completely sealed, providing maximum protection to the user and the environment.

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HEPA filter

A high-efficiency particulate air filter that removes small particles from the air.

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BSC use procedure - step 1

Turn on the fan and lights, turn off UV light, and ensure drain valve is closed.

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BSC use procedure - step 2

Disinfect the work surface with a solution like 70% ethanol.

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Biological Safety Cabinet Pre-Work

Allow the biological safety cabinet (BSC) to run for 5-10 minutes to clean itself before starting work.

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BSC Work Area Organization

Plan work, place materials in the cabinet before starting, properly segregate materials (clean/contaminated).

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BSC Airflow Disruption Prevention

Avoid placing materials on the air intake grille, keep materials/equipment inside the cabinet work area, perform manipulations far back.

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BSC Decontamination

After procedures, decontaminate all equipment (disinfect), run cabinet for 3 minutes without operation to purge contaminants.

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BSC Post-Procedure Cleaning

Wipe surfaces with disinfectant (70% ethanol or other). Clean up spills to prevent future contamination.

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BSC Access Limitation

Limit access to one person at a time to maintain proper airflow and to prevent contamination.

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BSC Open Flame Prohibition

Never use open flame inside the cabinet; it damages HEPA filters and disrupts the airflow.

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BSC Work Pattern

Conduct research in a 'clean to dirty' (left to right) work pattern to maintain a safe workflow.

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What is an incubator?

An insulated box with adjustable heating, typically up to 60-100°C, used to grow and maintain bacteria, fungi, or cell cultures at a specific temperature.

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What are the key components of an incubator?

Components typically include an air circulating fan, thermostat, timer, thermometer, electric heater, and sometimes inner glass doors.

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What is a standard incubator?

A basic incubator that uses gravity or fan assisted convection to heat, with a temperature range from ambient to 80-100°C.

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Why are CO2 incubators important?

They are used to maintain and grow biological samples that require a constant pressure of CO2 in the air, usually between 5-20%.

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What makes a humidity incubator special?

It controls both temperature and humidity levels, and uses a water tray to create a humid environment.

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What does a shaking incubator do?

It shakes the contents in a temperature-controlled atmosphere. This is useful for mixing substances.

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Why is a constant 37°C important for incubators?

It's the optimal temperature for the growth of many human cells and bacteria.

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What is the incubator used for?

Incubators have diverse applications in biology, including growing bacteria, fungi, cells, and tissues to study their characteristics or for various research purposes.

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Study Notes

Lab Instrument: Incubator & Biological Safety Cabinet

  • Incubators: Insulated boxes with adjustable heaters
  • Temperature range: 60 to 100°C
  • Used in medical and other labs to grow and maintain bacteria, fungi, or cell cultures.
  • Ideal temperature for cells/tissue cultures: 37°C
  • Cultivation time: 18-24 hours
  • Can be used for unicellular and multicellular organisms

Incubator Growth Factors

  • Constant temperature
  • Humidity typically >80% to prevent evaporation
  • Carbon dioxide (CO2) level of 5% to maintain a slightly acidic pH
  • Oxygen to maintain life

Incubator Components/Parts

  • Thermometer
  • Double-walled insulated chamber
  • Air circulating fan
  • Perforated tray
  • Temperature regulator
  • Power indicator switch
  • Heating switch
  • Main switch
  • Asbestos door gasket

Main Parts of Laboratory Incubators

  • Air circulating fan
  • Thermostat
  • Timer
  • Thermometer
  • Electric heater
  • Some have inner glass doors for viewing without disrupting the atmosphere.

General Types of Incubators

  • Standard: Gravity or fan-assisted convection, to 80°C - 100°C
  • Cooled: Work at or below ambient temperature.
  • Humidity: Control both temperature and humidity; water tray for constant evaporation.
  • CO2 (Carbon dioxide): Maintain constant CO2 pressure, 5-20%
  • Shaking: Temperature controlled atmosphere for shaking samples

Uses of Laboratory Incubators

  • Biology: Cell and tissue cultures (extracting tissue, controlled environments for growing)
  • Molecular biology
  • Microbiology
  • Biochemical and hematological studies
  • Pharmaceuticals and hematological studies
  • Food analysis

Biological Safety Cabinets (BSCs)

  • Ventilated enclosures protecting users, products, and environments from aerosols.
  • Continuous airflow discharged through high-efficiency particulate air (HEPA) filters and high-energy UV light.
  • Killing live bacteria and viruses

Uses of Protection Cabinets

  • Personal protection from harmful agents
  • Product protection from contamination
  • Environmental protection from contaminants

Classification of Biological Cabinets

  • Class I: Personnel and environmental protection, no product protection
  • Class II: Partial barrier systems; air moves to protect all three (personnel, environment, product)
  • Class III: Gas-tight maximum protection for personnel, product, and environment

Class 1 Biological Safety Cabinets Features

  • Front opening
  • Sash
  • Exhaust HEPA filter
  • Exhaust plenum

Class 2 Biological Safety Cabinets Features

  • Front opening
  • Sash
  • Exhaust HEPA filter
  • Supply HEPA filter
  • Common plenum
  • Blower

Class 3 Biological Safety Cabinets Features

  • Front opening
  • Sash
  • Supply HEPA filter
  • Blower

Procedures for Proper BSC Use

  • Turn on fan and light, turn off UV. Close drain.
  • Wipe surface with 70% ethanol or disinfectant for 5-10min.
  • Plan work in advance, place all tools/materials inside.
  • Arrange materials logically; segregate clean and contaminated materials.
  • Remove unnecessary materials immediately.
  • Avoid materials on the intake grille (impacts protective barrier).
  • Keep equipment inside the cabinet area.
  • Decontaminate equipment in direct contact with the agent.
  • Run the cabinet 3 minutes post-procedure for decontamination.
  • Wipe surfaces with ethanol, clean spilled culture.
  • Some researchers choose to run the fan continuously.
  • Limit access to one person at a time; negates protective air barrier.
  • Do not use open flames; damages HEPA filter.
  • Conduct research in a "clean to dirty" pattern.

Biohazard Spill Control Inside BSC

  • Keep cabinet on
  • Wear gloves
  • Spray wipe walls, surfaces, and equipment with decontamination solution
  • Use drain pans and catch basins
  • Allow solution to sit for 20 min
  • Drain the solution
  • Lift out and clean any removable surfaces
  • Clean all surfaces with decontamination solution

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Related Documents

Lab 3. Incubator & Biological Safety Cabinet PDF

Description

Explore the essential features and components of incubators and biological safety cabinets. Learn about their importance in laboratory settings, specifically for growing bacteria, fungi, and cell cultures. This quiz covers the functions and parts that make up these vital instruments.

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