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What is the primary function of a Biological Safety Cabinet?
What is the primary function of a Biological Safety Cabinet?
Class I Biological Safety Cabinets have a front opening and an exhaust HEPA filter.
Class I Biological Safety Cabinets have a front opening and an exhaust HEPA filter.
True
What should be done to disinfect the work surface of a Biological Safety Cabinet?
What should be done to disinfect the work surface of a Biological Safety Cabinet?
Wipe the work surface with 70% ethanol or other appropriate disinfectant.
The continuous airflow in a Biological Safety Cabinet is released through a __________ filter.
The continuous airflow in a Biological Safety Cabinet is released through a __________ filter.
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Match the following types of Biological Safety Cabinets with their features:
Match the following types of Biological Safety Cabinets with their features:
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Which of the following is NOT a protection mechanism of Biological Safety Cabinets?
Which of the following is NOT a protection mechanism of Biological Safety Cabinets?
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The Class II Biological Safety Cabinet includes a common plenum and a blower.
The Class II Biological Safety Cabinet includes a common plenum and a blower.
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List one application of tissue culture techniques.
List one application of tissue culture techniques.
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Which type of incubator is designed to work at temperatures close to or below ambient temperature?
Which type of incubator is designed to work at temperatures close to or below ambient temperature?
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Carbon dioxide incubators maintain a CO2 concentration in air ranging from 5% to 20%.
Carbon dioxide incubators maintain a CO2 concentration in air ranging from 5% to 20%.
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What should be done before beginning work in a Biological Safety Cabinet (BSC)?
What should be done before beginning work in a Biological Safety Cabinet (BSC)?
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It is safe to use an open flame inside a Biological Safety Cabinet.
It is safe to use an open flame inside a Biological Safety Cabinet.
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Name one use of laboratory incubators in biological applications.
Name one use of laboratory incubators in biological applications.
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What disinfectant concentration is recommended for cleaning the work surface of a BSC after use?
What disinfectant concentration is recommended for cleaning the work surface of a BSC after use?
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A __________ incubator is used to control both temperature and humidity for biological samples.
A __________ incubator is used to control both temperature and humidity for biological samples.
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What is a main feature of standard incubators?
What is a main feature of standard incubators?
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After completing work in a BSC, run the cabinet for at least ______ minutes with no activity to purge airborne contaminants.
After completing work in a BSC, run the cabinet for at least ______ minutes with no activity to purge airborne contaminants.
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Match the following types of incubators with their descriptions:
Match the following types of incubators with their descriptions:
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Match the following procedures with their corresponding purpose in a Biological Safety Cabinet:
Match the following procedures with their corresponding purpose in a Biological Safety Cabinet:
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Which of the following is NOT recommended in a Biological Safety Cabinet?
Which of the following is NOT recommended in a Biological Safety Cabinet?
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Which part of an incubator is responsible for circulating air?
Which part of an incubator is responsible for circulating air?
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Two people can safely work in a BSC at the same time without impacting the safety measures.
Two people can safely work in a BSC at the same time without impacting the safety measures.
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Biological safety cabinets are classified based on their ability to maintain a sterile environment.
Biological safety cabinets are classified based on their ability to maintain a sterile environment.
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What is the primary purpose of running the Biological Safety Cabinet for 5-10 minutes before starting work?
What is the primary purpose of running the Biological Safety Cabinet for 5-10 minutes before starting work?
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Study Notes
Lab Instrument: Incubator & Biological Safety Cabinet
- Incubators: Insulated boxes with adjustable heaters
- Temperature range: 60 to 100°C
- Used in medical and other labs to grow and maintain bacteria, fungi, or cell cultures.
- Ideal temperature for cells/tissue cultures: 37°C
- Cultivation time: 18-24 hours
- Can be used for unicellular and multicellular organisms
Incubator Growth Factors
- Constant temperature
- Humidity typically >80% to prevent evaporation
- Carbon dioxide (CO2) level of 5% to maintain a slightly acidic pH
- Oxygen to maintain life
Incubator Components/Parts
- Thermometer
- Double-walled insulated chamber
- Air circulating fan
- Perforated tray
- Temperature regulator
- Power indicator switch
- Heating switch
- Main switch
- Asbestos door gasket
Main Parts of Laboratory Incubators
- Air circulating fan
- Thermostat
- Timer
- Thermometer
- Electric heater
- Some have inner glass doors for viewing without disrupting the atmosphere.
General Types of Incubators
- Standard: Gravity or fan-assisted convection, to 80°C - 100°C
- Cooled: Work at or below ambient temperature.
- Humidity: Control both temperature and humidity; water tray for constant evaporation.
- CO2 (Carbon dioxide): Maintain constant CO2 pressure, 5-20%
- Shaking: Temperature controlled atmosphere for shaking samples
Uses of Laboratory Incubators
- Biology: Cell and tissue cultures (extracting tissue, controlled environments for growing)
- Molecular biology
- Microbiology
- Biochemical and hematological studies
- Pharmaceuticals and hematological studies
- Food analysis
Biological Safety Cabinets (BSCs)
- Ventilated enclosures protecting users, products, and environments from aerosols.
- Continuous airflow discharged through high-efficiency particulate air (HEPA) filters and high-energy UV light.
- Killing live bacteria and viruses
Uses of Protection Cabinets
- Personal protection from harmful agents
- Product protection from contamination
- Environmental protection from contaminants
Classification of Biological Cabinets
- Class I: Personnel and environmental protection, no product protection
- Class II: Partial barrier systems; air moves to protect all three (personnel, environment, product)
- Class III: Gas-tight maximum protection for personnel, product, and environment
Class 1 Biological Safety Cabinets Features
- Front opening
- Sash
- Exhaust HEPA filter
- Exhaust plenum
Class 2 Biological Safety Cabinets Features
- Front opening
- Sash
- Exhaust HEPA filter
- Supply HEPA filter
- Common plenum
- Blower
Class 3 Biological Safety Cabinets Features
- Front opening
- Sash
- Supply HEPA filter
- Blower
Procedures for Proper BSC Use
- Turn on fan and light, turn off UV. Close drain.
- Wipe surface with 70% ethanol or disinfectant for 5-10min.
- Plan work in advance, place all tools/materials inside.
- Arrange materials logically; segregate clean and contaminated materials.
- Remove unnecessary materials immediately.
- Avoid materials on the intake grille (impacts protective barrier).
- Keep equipment inside the cabinet area.
- Decontaminate equipment in direct contact with the agent.
- Run the cabinet 3 minutes post-procedure for decontamination.
- Wipe surfaces with ethanol, clean spilled culture.
- Some researchers choose to run the fan continuously.
- Limit access to one person at a time; negates protective air barrier.
- Do not use open flames; damages HEPA filter.
- Conduct research in a "clean to dirty" pattern.
Biohazard Spill Control Inside BSC
- Keep cabinet on
- Wear gloves
- Spray wipe walls, surfaces, and equipment with decontamination solution
- Use drain pans and catch basins
- Allow solution to sit for 20 min
- Drain the solution
- Lift out and clean any removable surfaces
- Clean all surfaces with decontamination solution
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Description
Explore the essential features and components of incubators and biological safety cabinets. Learn about their importance in laboratory settings, specifically for growing bacteria, fungi, and cell cultures. This quiz covers the functions and parts that make up these vital instruments.
