Biological Safety Cabinet (BSC) Cleaning and Disinfection Procedures

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Which type of biological safety cabinet is 100% exhaust ventilated?

Class II type B2 (correct)

BSL Risk Level 3-4

BSL Risk Level 1-3

Class II type B1

What is the purpose of a class II type B1 biological safety cabinet?

Enclosing workstations on three sides

Exhausting all air through a HEPA filter to the outside

Protecting the samples from environmental contamination (correct)

Providing positive pressure Personnel and Product protection

What is a typical use of Class II type B2 biological safety cabinets?

Testing the BSC's integrity

Handling of bacterial, viral, and fungal samples (correct)

Recirculating air back into the lab

UV lamp disinfection

How are potentially dangerous samples and contamination protected in BSL Risk Level 1-3 settings?

Using Class II type B2 biological safety cabinets Signup and view all the answers

What inflow velocity of air is drawn in through a HEPA filter in a Class II type B1 cabinet?

100 ft/min Signup and view all the answers

What is the purpose of a Class I biosafety cabinet (BSC)?

To vent HEPA-filtered air outside to remove contaminants Signup and view all the answers

Which type of biosafety cabinet (BSC) is best suited for applications requiring sterile conditions without the need to work with volatile chemicals?

Class II Type A1 Signup and view all the answers

What is the primary function of a Class II B biosafety cabinet (BSC)?

To recirculate HEPA-filtered air within the cabinet Signup and view all the answers

Which biosafety cabinet (BSC) type has an inflow velocity requirement of 100 fpm minimum?

Class II Type A2 Signup and view all the answers

What is the main drawback of using a Class II Type A1 biosafety cabinet for working with volatile chemicals?

Does not protect against vapors and gases Signup and view all the answers

In biosafety cabinets, what is the purpose of HEPA filters?

To remove contaminants from the air before returning it to the lab or venting it outside Signup and view all the answers

Which of the following statements about UV lamps in a biosafety cabinet (BSC) is correct?

UV lamps may cause performance degradation of the BSC over time. Signup and view all the answers

What is the recommended procedure for cleaning and disinfecting the interior surfaces of a BSC?

Use a cloth dampened with water and soap, followed by a disinfecting solution. Signup and view all the answers

What is the purpose of the HEPA filter leak test (LT) for a BSC?

To check the HEPA filters, filter housing, and mounting frames for possible leakage. Signup and view all the answers

What is the acceptance criteria for the HEPA filter leak test (LT) in a BSC?

The leak should be not more than 0.01% of the specific before the filter. Signup and view all the answers

What is the purpose of the inflow velocity test (IV) for a BSC?

To determine the nominal value of the inflow velocity (air entering the BSC). Signup and view all the answers

What is the acceptance criteria for the inflow velocity test (IV) in a BSC?

The nominal value should be 0.45 m/s. Signup and view all the answers