Biological Safety Cabinet Overview
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Biological Safety Cabinet Overview

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Questions and Answers

What is the recommended preservation method for urine when a delay in testing is anticipated?

  • Refrigeration at 4°C (correct)
  • Room temperature storage
  • Freezing at -20°C
  • Chemical preservation with formalin
  • What is the maximum recommended time for transporting biological specimens to the laboratory after collection?

  • 1 hour
  • 3 hours
  • 2 hours (correct)
  • 5 hours
  • Which of the following represents a basis for specimen rejection?

  • Specimen collection exceeded 30 minutes
  • Specimen is leaking (correct)
  • Specimen is positive for pathogens
  • Specimen temperature exceeds 25°C
  • What is the significance of the biohazard symbol created by Charles Baldwin?

    <p>It alerts personnel to potential infectious materials</p> Signup and view all the answers

    What is one of the essential components that a collection manual must include?

    <p>Sterile vs. non-sterile specimen handling procedures</p> Signup and view all the answers

    What is the primary role of the Occupational Safety and Health Administration (OSHA) in a clinical laboratory?

    <p>To promote workplace safety and health.</p> Signup and view all the answers

    Which of the following best describes the function of biological safety cabinets (BSCs)?

    <p>To safeguard laboratory personnel and samples from biological hazards.</p> Signup and view all the answers

    What is an essential practice for preventing biological contamination in the laboratory?

    <p>Regularly cleaning and maintaining all equipment.</p> Signup and view all the answers

    What is the significance of organizing materials within a biosafety cabinet?

    <p>To ensure optimal airflow and reduce cross-contamination.</p> Signup and view all the answers

    Which of the following accurately reflects the principle of biosafety?

    <p>Biosafety protects people from germs.</p> Signup and view all the answers

    Study Notes

    Biological Safety Cabinet

    • A device that protects workers from aerosol exposure and infectious disease agents.
    • Sterilizes air using heat, UV light, or a HEPA filter.
    • Three types of BSC: Class I, Class II, and Class III.

    Class I Cabinet

    • Open-fronted, negative pressure, ventilated cabinet.
    • Allows room air to enter the cabinet, circulate, and expose the material within.
    • Only exhaust air is filtered using a HEPA filter.
    • Used for BSL 2 and 3 agents.

    Class II Cabinet

    • Also known as laminar flow BSC.
    • Commonly used in clinical microbiology laboratories.
    • Sterilizes air using a HEPA filter.
    • Air flows directly over infectious material and is exhausted.
    • Used for BSL 2 and 3 agents.
    • Two types:
      • Class IIA - has fixed openings; 70% of air is recirculated.
      • Class IIB - variable sash opening; used for chemicals, radioisotopes, and carcinogens.

    Class III Cabinet

    • Provides the highest level of safety to the worker.
    • Air entering and exiting the cabinet is HEPA filtered.
    • Infectious material within the cabinet is handled with rubber gloves attached and sealed to the cabinet.

    OSHA Pathogen Regulations

    • Universal Precautions: Treating all blood, tissue, body fluids, and OPIM (other potentially infectious materials) as infectious.
    • Engineering and Work Practice Controls: Physical steps to isolate or remove potential pathogen hazards from the workplace. Examples: Sharp’s containers, biological safety cabinets, laboratory fume hoods, proper hand washing, banning of eating, smoking, and drinking.
    • Personal Protective Equipment: Gloves, eye and face protection, protective body clothing.

    Biological Contamination

    • Contamination of food or environment with microorganisms (bacteria, viruses, fungi, and parasites).
    • Describes the event where foreign materials or substances can cause foodborne illnesses enter food materials.
    • Contaminated sites can impact human health and the environment based on the type of contaminant, concentration, location, exposure mechanism, and exposure level.

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    Description

    Explore the essentials of Biological Safety Cabinets (BSC), including their types and functions. This quiz covers Class I, II, and III cabinets and their specific applications in laboratory settings. Test your knowledge on the safe handling of infectious materials and protective equipment.

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