Bioenergetics and Cellular Respiration Overview
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Questions and Answers

What are the two compartments formed by the double-membrane mitochondrion?

  • Nucleus and cytoplasm
  • Intermembrane space and cristae
  • Inner membrane and outer membrane (correct)
  • Cytoplasmic fluid and matrix
  • Which of the following stages of aerobic respiration occurs first?

  • Formation of acetyl-CoA
  • Glycolysis (correct)
  • Krebs cycle
  • Electron transport chain
  • What is the main product of glycolysis?

  • NADH
  • Pyruvate (correct)
  • Glucose
  • Acetyl-CoA
  • Which phrase describes the Energy Investment Phase of glycolysis?

    <p>Requires an investment of ATP (C)</p> Signup and view all the answers

    What happens to pyruvate during the formation of acetyl-CoA?

    <p>It is converted to acetyl-CoA and CO2 is released (D)</p> Signup and view all the answers

    In aerobic respiration, what is produced as a direct result of glucose oxidation?

    <p>Energy in the form of ATP (D)</p> Signup and view all the answers

    Which of the following correctly describes the role of NAD+ in glycolysis?

    <p>Serves as an electron acceptor (D)</p> Signup and view all the answers

    Where does glycolysis occur within a cell?

    <p>In the cytoplasmic fluid (B)</p> Signup and view all the answers

    What is produced when a coenzyme-A is attached to pyruvate?

    <p>Acetyl-CoA (B)</p> Signup and view all the answers

    How many NADH molecules are produced during one cycle of the Krebs cycle per glucose molecule?

    <p>6 (B)</p> Signup and view all the answers

    Which molecule is regenerated at the end of the Krebs cycle?

    <p>Oxaloacetate (D)</p> Signup and view all the answers

    What type of reactions do the electron transport chain involve?

    <p>Reduction-oxidation (redox) (B)</p> Signup and view all the answers

    Which component carries high-energy electrons to the electron transport chain?

    <p>FADH2 (C)</p> Signup and view all the answers

    Who received the Nobel Prize for the identification of the Krebs cycle?

    <p>Hans Adolf Krebs (C)</p> Signup and view all the answers

    In which part of the cell does the Krebs cycle occur?

    <p>Matrix of mitochondria (D)</p> Signup and view all the answers

    What is formed when a four-carbon molecule oxaloacetate accepts a two-carbon acetyl group?

    <p>Citric acid (A)</p> Signup and view all the answers

    What is the total maximum ATP yield from one glucose molecule during aerobic respiration?

    <p>36 to 38 ATP (B)</p> Signup and view all the answers

    How much ATP does the oxidation of FADH2 yield per molecule?

    <p>2 ATP (C)</p> Signup and view all the answers

    Which process occurs in the absence of oxygen to generate ATP?

    <p>Fermentation (A)</p> Signup and view all the answers

    What is the primary function of NADH produced during glycolysis?

    <p>Transfer electrons during fermentation (C)</p> Signup and view all the answers

    What is the yield of ATP from the two NADH molecules produced in glycolysis in eukaryotes?

    <p>2 ATP (D)</p> Signup and view all the answers

    Which organic molecule can enter glycolysis after being converted to pyruvate?

    <p>Glutamate (C)</p> Signup and view all the answers

    What is the caloric value of one gram of lipid in the diet?

    <p>9 kcal (B)</p> Signup and view all the answers

    Which of the following statements about the Krebs cycle is accurate?

    <p>It generates both NADH and FADH2. (C)</p> Signup and view all the answers

    Which enzyme complex is responsible for reducing NADH?

    <p>NADH-Q reductase complex (A)</p> Signup and view all the answers

    Which of the following electron carriers can exist in both oxidized and reduced forms?

    <p>All of the above (D)</p> Signup and view all the answers

    What is the primary function of flavin mononucleotide (FMN) in the electron transport chain?

    <p>Transfers electrons from NADH-Q reductase to the next complex (A)</p> Signup and view all the answers

    Which complex is located after the cytochrome b/c1 reductase complex in the electron transport chain?

    <p>Cytochrome c oxidase complex (A)</p> Signup and view all the answers

    What process is represented by the equation 2 H+ + 1/2 O2 → H2O?

    <p>Reduction of oxygen (B)</p> Signup and view all the answers

    Which of the following complexes directly contributes to the generation of a proton gradient?

    <p>Cytochrome c oxidase complex (C)</p> Signup and view all the answers

    Which of the following statements about the electron transport chain is false?

    <p>Ubiquinone is a fixed component of the electron transport chain. (C)</p> Signup and view all the answers

    What is the end product when electrons are finally transferred to oxygen in the electron transport chain?

    <p>Water (B)</p> Signup and view all the answers

    What is the primary purpose of cellular respiration in cells?

    <p>To generate ATP for cellular work (A)</p> Signup and view all the answers

    Which type of organism can switch from aerobic respiration to fermentation when oxygen is unavailable?

    <p>Facultative anaerobe (B)</p> Signup and view all the answers

    In cellular respiration, what is the role of the final electron acceptor when it is oxygen?

    <p>It gets reduced (D)</p> Signup and view all the answers

    What is the chemical equation for the process of cellular respiration using glucose as fuel?

    <p>C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy (C)</p> Signup and view all the answers

    What is the primary role of NADH and FADH2 in the electron transport chain?

    <p>To donate electrons with high energy content. (B)</p> Signup and view all the answers

    What is often referred to as the 'power station' of the cell?

    <p>Mitochondrion (B)</p> Signup and view all the answers

    Which process couples electron transport to ATP synthesis?

    <p>Oxidative phosphorylation. (B)</p> Signup and view all the answers

    What is the final electron acceptor in the electron transport chain?

    <p>Oxygen (O2) (B)</p> Signup and view all the answers

    What is produced alongside ATP during cellular respiration?

    <p>NADH (D)</p> Signup and view all the answers

    Which of the following processes occurs in the mitochondria?

    <p>Cellular respiration (C)</p> Signup and view all the answers

    What is formed as electrons from NADH or FADH2 lose energy while moving through the electron transport chain?

    <p>A proton gradient. (B)</p> Signup and view all the answers

    What type of respiration occurs when there is no oxygen available?

    <p>Anaerobic respiration (A)</p> Signup and view all the answers

    How does the proton gradient contribute to ATP synthesis?

    <p>It powers ATP synthesis as protons diffuse back into the matrix. (C)</p> Signup and view all the answers

    What type of process is the diffusion of protons through ATP synthase?

    <p>Exergonic process. (C)</p> Signup and view all the answers

    In which part of the cell does oxidative phosphorylation primarily occur?

    <p>Mitochondria. (A)</p> Signup and view all the answers

    What is the role of ATP synthase in the process of ATP production?

    <p>To synthesize ATP using the energy from the proton gradient. (A)</p> Signup and view all the answers

    Flashcards

    Bioenergetics

    The study of how organisms manage their energy resources.

    Cellular Respiration

    Process where cells break down glucose to generate ATP, the cell's energy currency.

    Photosynthesis

    Process where plants use sunlight to convert carbon dioxide and water into sugar and oxygen.

    Aerobic

    An organism that requires oxygen to survive and thrive.

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    Anaerobic

    An organism that can live without oxygen.

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    Facultative Anaerobe

    Organism that can survive with or without oxygen.

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    Mitochondrion

    The powerhouse of the cell, where cellular respiration takes place.

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    ATP

    The main energy currency of cells, used for various cellular processes.

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    Electron Transport Chain

    A series of protein complexes and electron carriers embedded in the inner mitochondrial membrane that transfer electrons from NADH and FADH2 to molecular oxygen, generating a proton gradient used for ATP synthesis.

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    Electron Carriers

    Molecules that can accept and donate electrons, facilitating the flow of electrons through the electron transport chain.

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    Oxidized and Reduced Forms

    Electron carriers can exist in two states: oxidized (missing electrons) and reduced (gained electrons). They cycle between these states during electron transfer.

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    Redox Reactions

    Chemical reactions involving the transfer of electrons from a donor (reducing agent) to an acceptor (oxidizing agent).

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    Complex I

    NADH-Q reductase complex. It accepts electrons from NADH and passes them to ubiquinone (Q).

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    Complex II

    Succinate-Q reductase complex. It accepts electrons from FADH2 and passes them to ubiquinone (Q).

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    Complex III

    Cytochrome b/c1 reductase complex. It accepts electrons from ubiquinone (Q) and passes them to cytochrome c.

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    Complex IV

    Cytochrome c oxidase complex. It accepts electrons from cytochrome c and passes them to molecular oxygen, producing water. This is where the majority of the energy is released.

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    Pyruvate Dehydrogenase

    An enzyme that catalyzes the conversion of pyruvate to acetyl-CoA, a key step in cellular respiration.

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    Acetyl-CoA

    A molecule that enters the Krebs cycle and carries two carbon atoms from pyruvate.

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    Krebs Cycle

    A series of reactions that completes the breakdown of glucose, generating energy carriers like NADH and FADH2.

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    Citric Acid Cycle

    Another name for the Krebs cycle, named after the first molecule produced.

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    Oxaloacetate

    A four-carbon molecule that starts the Krebs cycle and is regenerated at the end.

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    Mitochondrial Membrane

    The inner membrane of mitochondria where the electron transport chain is located.

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    Proton Gradient

    An uneven distribution of protons (H+) across the inner mitochondrial membrane, with a higher concentration in the intermembrane space than in the matrix.

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    Chemiosmosis

    The process of using the energy stored in the proton gradient to synthesize ATP.

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    ATP Synthase

    An enzyme complex that acts as a channel for protons to flow back into the matrix, using the energy to synthesize ATP.

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    Oxidative Phosphorylation

    The production of ATP through a series of redox reactions in the electron transport chain, coupled with the movement of protons across the inner mitochondrial membrane.

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    Energy Loss

    As electrons move through the electron transport chain, they lose energy at each step.

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    Final Electron Acceptor

    Oxygen (O2) is the final acceptor of electrons in the electron transport chain, forming water (H2O).

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    Role of NADH and FADH2

    NADH and FADH2 are the primary electron donors in the electron transport chain, carrying high-energy electrons from metabolic reactions.

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    Mitochondrial Compartments

    The double-membrane mitochondrion creates four distinct compartments: the outer membrane, inner membrane, intermembrane space, and matrix. The inner membrane folds into cristae, increasing surface area for ATP production.

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    Aerobic Respiration Equation

    The complete breakdown of glucose in the presence of oxygen produces carbon dioxide, water, and energy stored in ATP. The overall equation is: C6H12O6 + 6 O2 + 6 H2O → 6 CO2 + 12 H2O + Energy (ATP)

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    Stages of Aerobic Respiration

    Aerobic respiration involves four main stages: glycolysis, formation of acetyl-CoA, Krebs cycle, and electron transport chain/chemiosmosis. Each stage extracts energy from glucose in a step-by-step process.

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    Glycolysis: What happens?

    Glycolysis is the initial breakdown of glucose into two molecules of pyruvate. This process occurs in the cytoplasm and doesn't require oxygen.

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    Glycolysis Phases

    Glycolysis has two phases: an energy investment phase where ATP is used and an energy liberation phase where ATP and NADH are produced.

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    Glycolysis: Outputs

    Glycolysis produces 2 pyruvate molecules, 2 ATP molecules, and 2 NADH molecules. These products are then used in the subsequent stages of respiration.

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    Formation of Acetyl-CoA: What happens?

    Pyruvate is converted into acetyl-CoA in the mitochondria. This involves removing a carbon atom from pyruvate as carbon dioxide.

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    Formation of Acetyl-CoA: Where?

    The conversion of pyruvate to acetyl-CoA takes place inside the mitochondria, specifically in the mitochondrial matrix.

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    ATP Yield from Glucose

    Aerobic respiration of one glucose molecule yields a maximum of 36 to 38 ATP. This includes ATP produced during glycolysis, pyruvate grooming, the Krebs cycle, and oxidative phosphorylation.

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    NADH and FADH2

    NADH and FADH2 are electron carriers that are produced during glycolysis, pyruvate grooming, and the Krebs cycle. They donate electrons to the electron transport chain, generating a proton gradient that drives ATP synthesis.

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    Fermentation

    An anaerobic process that regenerates NAD+ by transferring electrons from NADH to an organic molecule. This allows glycolysis to continue in the absence of oxygen.

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    Other Fuels for Respiration

    Besides glucose, cells can use carbohydrates, proteins, and fats as fuel sources. These molecules are broken down into intermediates that enter glycolysis or the Krebs cycle.

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    Glycolysis

    The first stage of cellular respiration, occurring in the cytoplasm. Glucose is broken down into pyruvate, generating a small amount of ATP and NADH.

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    Study Notes

    Bioenergetics Overview

    • Covers energy, cellular respiration, and photosynthesis
    • Relates energy transfer within ecosystems

    Cellular Respiration

    • Aerobic respiration: Requires oxygen; produces ATP from glucose.
    • Anaerobic respiration: Does not require oxygen; uses an inorganic molecule as the final electron acceptor instead.
    • Fermentation: Uses an organic molecule as the final electron acceptor to regenerate NAD+ for glycolysis.

    Mitochondrion Structure and Function

    • Double-membrane structure with compartments:
      • Outer membrane
      • Inner membrane
      • Intermembrane space
      • Matrix
      • Cristae (folds in inner membrane)

    Aerobic Respiration (Redox Process)

    • Consists of four main stages:
      • Glycolysis
      • Formation of Acetyl-CoA
      • Krebs cycle
      • Electron transport chain/Chemiosmosis
    • The overall reaction pathway for glucose is: C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + Energy
    • Oxidation and reduction occur during the process

    Glycolysis

    • Breakdown of glucose into pyruvate (in the cytosol)
    • Two phases:
      • Energy investment phase (requires ATP)
      • Energy liberation phase (produces ATP and NADH)
    • Net gain of 2 ATP, 2 NADH, and 2 Pyruvate

    Formation of Acetyl-CoA

    • Occurs in the mitochondria
    • Pyruvate is converted to Acetyl-CoA
    • Generates NADH and releases CO₂

    Krebs Cycle (Tricarboxylic Acid Cycle)

    • Takes place in the mitochondrial matrix
    • Acetyl-CoA enters the cycle and combines with oxalacetate to form citrate.
    • Series of eight reactions that complete the breakdown of glucose to CO2.
    • Produces 2 ATP, 6 NADH, and 2 FADH2 per glucose molecule

    Electron Transport Chain

    • Complex series of electron carriers embedded in the inner mitochondrial membrane.
    • Electrons from NADH and FADH2 are passed along the chain releasing energy.
    • The energy is used to pump H+ ions into the intermembrane space.
    • This creates a gradient that drives ATP synthesis.

    Chemiosmosis

    • Process of ATP synthesis fueled by the H+ gradient.
    • H+ ions diffuse back into the matrix through ATP synthase.
    • This drives the production of ATP from ADP.

    Oxidative Phosphorylation

    • Coupled to the electron transport chain.
    • Most ATP synthesis occurs in this process.
    • ATP synthase is the enzyme responsible for ATP production

    Summary of Aerobic Respiration ATP Production

    • Glycolysis: 2 ATP
    • Pyruvate grooming: 2 NADH
    • Krebs cycle: 2 ATP + 6 NADH + 2 FADH₂
    • Total ATP per glucose molecule (~36-38 ATP) *Depending on the shuttle system

    Fermentation

    • Alternative energy production in the absence of oxygen.
    • Two common types:
      • Alcoholic fermentation
      • Lactic acid fermentation
    • Regenerates NAD+ to allow glycolysis to continue, enabling a low yield of ATP production through substrate-level phosphorylation.

    Other Organic Molecules as Fuels

    • Carbohydrates (glucose), proteins (amino acids), and fats (glycerol and fatty acids) can all be used as fuels for cellular respiration.
    • Each molecule has different entry points.
    • Different fuels will yield different amounts of ATP.

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    Cellular Respiration PDF

    Description

    This quiz covers essential concepts in bioenergetics, focusing on energy transfer in cellular respiration and photosynthesis. Key topics include the structure and function of mitochondria, types of respiration, and the stages of aerobic respiration. Test your understanding of these critical biological processes!

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