Biochemistry: Enzymes and Isoenzymes
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Questions and Answers

What is the main difference between isoenzymes?

  • They are found in different locations in the body. (correct)
  • They catalyze different reactions.
  • They are made of completely different amino acids.
  • They have different active sites.
  • Which of the following isoenzymes is found predominantly in the liver or skeletal tissues?

  • LDH5 (correct)
  • LDH3
  • LDH2
  • LDH1
  • How many subunits does the Lactate dehydrogenase (LDH) enzyme have?

  • 3
  • 4 (correct)
  • 2
  • 1
  • What is the primary function of enzymes?

    <p>To speed up the rate of chemical reactions. (B)</p> Signup and view all the answers

    Which isoenzyme of Creatine phosphokinase (CPK) is primarily found in the brain?

    <p>BB (B)</p> Signup and view all the answers

    Flashcards

    Enzymes

    Biological catalysts that speed up chemical reactions in the body.

    Isoenzymes

    Enzymes that catalyze the same reaction but differ in structure or composition.

    Lactate Dehydrogenase (LDH)

    A tetrameric enzyme with 5 forms, each found in different body tissues.

    Creatine Phosphokinase (CPK)

    A dimer with two types, BB for brain and MM for muscle, with 3 forms.

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    Enzyme function in diagnosis

    Certain enzymes are used clinically to diagnose organ damage.

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    Study Notes

    Enzymes in the Body

    • Enzymes are biological catalysts that accelerate chemical reactions in the body.
    • Isoenzymes catalyze the same reaction but have different structures/subunit compositions.
    • Enzymes are used in diagnostic medicine.

    Lactate Dehydrogenase (LDH)

    • LDH is a tetrameric enzyme with 2 subunits.
    • It exists in 5 forms (isoenzymes).
    • LDH 1 is found in cardiac cells.
    • LDH 2 is found in red blood cells.
    • LDH 3 is found in the lungs.
    • LDH 4 is found in the kidney/pancreatic cells.
    • LDH 5 is found in the liver/skeletal tissues.

    Creatine Phosphokinase (CPK)

    • CPK is a dimeric enzyme with protomers.
    • It exists in 3 forms (isoenzymes).
    • BB form is in the brain.
    • MM form is in muscle.

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    Description

    Explore the fascinating roles of enzymes in the body, including their functions as biological catalysts. This quiz delves into specific enzymes like Lactate Dehydrogenase and Creatine Phosphokinase, highlighting their different isoenzymes and tissue distributions. Test your understanding of these crucial biochemical components and their significance in diagnostic medicine.

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