Biochemistry 3: Enzymes (Part 2)
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Questions and Answers

What is the composition of the LDH enzyme?

  • Hexameric enzyme composed of three identical protein subunits
  • Monomeric enzyme composed of a single protein subunit
  • Tetrameric enzyme composed of two different protein subunits (correct)
  • Dimeric enzyme composed of two identical protein subunits

    • What is the name of the LDH isozyme found in skeletal muscle and liver?

  • LDH4
  • LDH5 (correct)
  • LDH1
  • LDH3

    • What is the name of the enzyme-linked immunosorbant assay?

  • EMSA
  • ELSA
  • EISA
  • ELISA (correct)

    • What is the principle of ELISA?

    <p>Linking an enzyme to an antibody</p> Signup and view all the answers

    What is the purpose of adding a substrate for the enzyme in ELISA?

    <p>To quantify the amount of antibody bound to the antigen</p> Signup and view all the answers

    Which isozyme is increased in leukemia?

    <p>All five isozymes</p> Signup and view all the answers

    Which enzyme is used as a diagnostic tool for myocardial infarction?

    <p>LDH</p> Signup and view all the answers

    What is the name of the enzyme used as a diagnostic tool for viral hepatitis?

    <p>SGPT</p> Signup and view all the answers

    Which enzyme is used as a diagnostic tool for bone disorders?

    <p>Alkaline phosphatase</p> Signup and view all the answers

    What is the purpose of spectrophotometric measurement in ELISA?

    <p>To quantify the amount of substrate converted</p> Signup and view all the answers

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