10 Questions
What is the composition of the LDH enzyme?
Tetrameric enzyme composed of two different protein subunits
What is the name of the LDH isozyme found in skeletal muscle and liver?
LDH5
What is the name of the enzyme-linked immunosorbant assay?
ELISA
What is the principle of ELISA?
Linking an enzyme to an antibody
What is the purpose of adding a substrate for the enzyme in ELISA?
To quantify the amount of antibody bound to the antigen
Which isozyme is increased in leukemia?
All five isozymes
Which enzyme is used as a diagnostic tool for myocardial infarction?
LDH
What is the name of the enzyme used as a diagnostic tool for viral hepatitis?
SGPT
Which enzyme is used as a diagnostic tool for bone disorders?
Alkaline phosphatase
What is the purpose of spectrophotometric measurement in ELISA?
To quantify the amount of substrate converted
This quiz covers types of enzyme inhibition, regulation of enzyme activity, and uses of enzymes, as taught by Dr. Samah Shehata from the Faculty of Physical Therapy.
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