Bacterial Growth Curve and DNA Replication
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Questions and Answers

What is the method used for direct cell counts that involves microbial suspension forced through a small orifice?

  • Electronic counters - Flow Cytometry (correct)
  • Counting chambers
  • Viable cell counts
  • Direct Counts on Membrane Filters
  • Which method is useful for counting bacteria and can distinguish living from dead cells?

  • Electronic counters - Flow Cytometry
  • Viable cell counts
  • Counting chambers
  • Direct Counts on Membrane Filters (correct)
  • What is the unit of measurement for viable cells expressing the population size as colony forming units?

  • Log N0
  • µ
  • Reciprocal of mean growth rate constant
  • CFU (correct)
  • Which method involves microbial cells being filtered through a special membrane to observe cells against a dark background?

    <p>Direct Counts on Membrane Filters (B)</p> Signup and view all the answers

    What is the term used to describe the method of plating dilutions of a population on suitable solid medium to calculate the number of cells in the population?

    <p>Spread plate and pour plate methods (D)</p> Signup and view all the answers

    Which method is easy, inexpensive, quick, but cannot distinguish living from dead cells?

    <p>Counting chambers (D)</p> Signup and view all the answers

    Which of the following terms describes organisms that require high osmotic pressure for growth?

    <p>Halophiles (A)</p> Signup and view all the answers

    A bacterial population increases from 10^5 cells to 10^11 cells in 15 hours. What is the generation time (g) of the bacteria?

    <p>3 hours (A)</p> Signup and view all the answers

    Which phase of the bacterial growth curve is characterized by the bacterial population remaining constant?

    <p>Stationary phase (B)</p> Signup and view all the answers

    What is the term used to describe bacteria that can survive in harsh conditions but are unable to be cultured using standard laboratory techniques?

    <p>Viable but non-culturable (D)</p> Signup and view all the answers

    Which of the following organisms is adapted to grow in low pH environments (pH 0-5.5)?

    <p>Acidophiles (A)</p> Signup and view all the answers

    What is the primary benefit that biofilms provide to the microbial species within the community?

    <p>Protection from harsh environmental conditions (A)</p> Signup and view all the answers

    What is the average time required for E. coli to replicate its DNA?

    <p>40 minutes (B)</p> Signup and view all the answers

    Which phase of the growth curve is characterized by a constant rate of growth?

    <p>Exponential phase (B)</p> Signup and view all the answers

    What is the maximum cell concentration that can be reached by bacteria in a batch culture?

    <p>$10^{10}$ cells/mL (D)</p> Signup and view all the answers

    Which of the following is NOT a possible reason for entry into the stationary phase?

    <p>Increase in cell division rate (A)</p> Signup and view all the answers

    Which protein assists RNA polymerase in transcribing genes for starvation proteins during the stationary phase?

    <p>RpoS (B)</p> Signup and view all the answers

    What is the term used to describe bacteria that are temporarily unable to grow but can resume growth when the environment becomes favorable?

    <p>Viable but non-culturable (VBNC) bacteria (D)</p> Signup and view all the answers

    What is the term used to describe the irreversible loss of a cell's ability to reproduce?

    <p>Loss of viability (C)</p> Signup and view all the answers

    Which phase of the growth curve is characterized by a plateau in the total number of viable cells?

    <p>Stationary phase (A)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of the exponential phase?

    <p>Decrease in population size (D)</p> Signup and view all the answers

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