Bacterial Gene Mutation Resistance Experiment

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What is the main purpose of this experiment?

To investigate the selective advantage of bacterial mutations

To detect resistance resulting from a mutation in a bacterial gene (correct)

To study the effects of streptomycin on bacterial protein synthesis

To compare the mutation rates in haploid and diploid organisms

Why are bacterial mutations more easily detectable compared to mutations in diploid organisms?

Bacteria multiply at a slower rate than diploid organisms

Bacteria have multiple, paired chromosomes

Recessive mutations occur in bacteria but not in diploid organisms

Bacteria have only one unpaired chromosome (correct)

What is the typical range of mutation rates in bacterial cells?

1 in 10^2 to 1 in 10^8 divisions

1 in 10^6 to 1 in 10^10 divisions

1 in 10^4 to 1 in 10^12 divisions (correct)

1 in 10^3 to 1 in 10^9 divisions

How do bacterial mutations provide a selective advantage?

By allowing the bacteria to survive in the presence of antimicrobial agents Signup and view all the answers

What is the role of streptomycin in this experiment?

It is used to inhibit protein synthesis in the bacterial cell Signup and view all the answers

Why is the combination of E. coli and streptomycin convenient for illustrating chromosomal resistance?

E. coli becomes resistant to streptomycin with just one mutation Signup and view all the answers

What is the purpose of adding histidine to the top agar in the Ames test?

To promote mutagenesis by allowing bacteria to undergo multiple cell divisions Signup and view all the answers

What might happen if the top agar is not vortexed properly before pouring over the glucose minimal salts agar?

Stippling of the top agar will occur Signup and view all the answers

What is the significance of a halo of densely packed revertant colonies around the disk in the Ames test?

It suggests the presence of mutagenic agent Signup and view all the answers

What could be the consequence of not moving quickly enough when adding bacteria to the top agar in the Ames test?

Stippling of the top agar Signup and view all the answers

Why is it necessary to incubate the plate at 37°C for 48 hours after adding the test reagent in the Ames test?

To allow time for mutation and colony formation Signup and view all the answers

Why are additional tester strains used in routine testing of the Ames test?

To accommodate different types of chemical compounds Signup and view all the answers

According to the passage, why are bacteria used for screening chemical compounds for potential carcinogenesis?

The fact that carcinogenic compounds induce increased rates of mutation in bacteria. Signup and view all the answers

What is the conventional method for determining if a chemical substance is carcinogenic?

Injecting the material into animals and looking for tumor development. Signup and view all the answers

What is the advantage of using the Ames test over the conventional animal testing method?

The Ames test is cheaper and easier to perform than animal testing. Signup and view all the answers

What is the role of the Ames test in determining carcinogenicity?

It serves as a screening test for detecting potentially carcinogenic compounds. Signup and view all the answers

What is the correlation between mutagenicity and carcinogenesis mentioned in the passage?

The correlation between mutagenicity and carcinogenesis is around 83%. Signup and view all the answers

What bacterial strain is used in the Ames test, and what is its characteristic?

A strain of Salmonella typhimurium that is auxotrophic for histidine. Signup and view all the answers

Bacterial Gene Mutation Resistance Experiment

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