Podcast
Questions and Answers
What is the main purpose of this experiment?
What is the main purpose of this experiment?
- To investigate the selective advantage of bacterial mutations
- To detect resistance resulting from a mutation in a bacterial gene (correct)
- To study the effects of streptomycin on bacterial protein synthesis
- To compare the mutation rates in haploid and diploid organisms
Why are bacterial mutations more easily detectable compared to mutations in diploid organisms?
Why are bacterial mutations more easily detectable compared to mutations in diploid organisms?
- Bacteria multiply at a slower rate than diploid organisms
- Bacteria have multiple, paired chromosomes
- Recessive mutations occur in bacteria but not in diploid organisms
- Bacteria have only one unpaired chromosome (correct)
What is the typical range of mutation rates in bacterial cells?
What is the typical range of mutation rates in bacterial cells?
- 1 in 10^2 to 1 in 10^8 divisions
- 1 in 10^6 to 1 in 10^10 divisions
- 1 in 10^4 to 1 in 10^12 divisions (correct)
- 1 in 10^3 to 1 in 10^9 divisions
How do bacterial mutations provide a selective advantage?
How do bacterial mutations provide a selective advantage?
What is the role of streptomycin in this experiment?
What is the role of streptomycin in this experiment?
Why is the combination of E. coli and streptomycin convenient for illustrating chromosomal resistance?
Why is the combination of E. coli and streptomycin convenient for illustrating chromosomal resistance?
What is the purpose of adding histidine to the top agar in the Ames test?
What is the purpose of adding histidine to the top agar in the Ames test?
What might happen if the top agar is not vortexed properly before pouring over the glucose minimal salts agar?
What might happen if the top agar is not vortexed properly before pouring over the glucose minimal salts agar?
What is the significance of a halo of densely packed revertant colonies around the disk in the Ames test?
What is the significance of a halo of densely packed revertant colonies around the disk in the Ames test?
What could be the consequence of not moving quickly enough when adding bacteria to the top agar in the Ames test?
What could be the consequence of not moving quickly enough when adding bacteria to the top agar in the Ames test?
Why is it necessary to incubate the plate at 37°C for 48 hours after adding the test reagent in the Ames test?
Why is it necessary to incubate the plate at 37°C for 48 hours after adding the test reagent in the Ames test?
Why are additional tester strains used in routine testing of the Ames test?
Why are additional tester strains used in routine testing of the Ames test?
According to the passage, why are bacteria used for screening chemical compounds for potential carcinogenesis?
According to the passage, why are bacteria used for screening chemical compounds for potential carcinogenesis?
What is the conventional method for determining if a chemical substance is carcinogenic?
What is the conventional method for determining if a chemical substance is carcinogenic?
What is the advantage of using the Ames test over the conventional animal testing method?
What is the advantage of using the Ames test over the conventional animal testing method?
What is the role of the Ames test in determining carcinogenicity?
What is the role of the Ames test in determining carcinogenicity?
What is the correlation between mutagenicity and carcinogenesis mentioned in the passage?
What is the correlation between mutagenicity and carcinogenesis mentioned in the passage?
What bacterial strain is used in the Ames test, and what is its characteristic?
What bacterial strain is used in the Ames test, and what is its characteristic?
