Podcast
Questions and Answers
What is the purpose of this lab?
What is the purpose of this lab?
To recognize bacterial flagella, describe their function, and understand motility techniques.
What does 'Flagellum' describe?
What does 'Flagellum' describe?
- Most common structure used by bacteria for motility
- Thin, hair-like protein extensions of bacterial cells
- Function - locomotion
- All of the above (correct)
What is the function of the flagella?
What is the function of the flagella?
Locomotion
The presence of flagella and their distribution on the cell can be useful in what two things?
The presence of flagella and their distribution on the cell can be useful in what two things?
Why is flagella a method of classification?
Why is flagella a method of classification?
What does 'Atrichous' mean?
What does 'Atrichous' mean?
What are the two main types of flagellar distribution?
What are the two main types of flagellar distribution?
What kind of flagella does 'Peritrichous' describe?
What kind of flagella does 'Peritrichous' describe?
What kind of flagella does 'Polar' describe?
What kind of flagella does 'Polar' describe?
What kind of polar flagella does 'Monotrichous' describe?
What kind of polar flagella does 'Monotrichous' describe?
What kind of polar flagella does 'Lophotrichous' describe?
What kind of polar flagella does 'Lophotrichous' describe?
What kind of polar flagella does 'Amphitrichous' describe?
What kind of polar flagella does 'Amphitrichous' describe?
What type of bacteria motility method involves staining?
What type of bacteria motility method involves staining?
What type of bacteria motility method includes the Hanging Drop technique?
What type of bacteria motility method includes the Hanging Drop technique?
What are three ways bacteria motility can be studied?
What are three ways bacteria motility can be studied?
What kind of bacteria motility technique can be directly observed with a light microscope?
What kind of bacteria motility technique can be directly observed with a light microscope?
Does this describe motile or non-motile bacteria in the Hanging Drop technique? They will seem to vibrate only within a confined location.
Does this describe motile or non-motile bacteria in the Hanging Drop technique? They will seem to vibrate only within a confined location.
What is Brownian movement?
What is Brownian movement?
Does this describe motile or non-motile bacteria in the Hanging Drop technique? Can be seen moving in a specific directional movement or flipping.
Does this describe motile or non-motile bacteria in the Hanging Drop technique? Can be seen moving in a specific directional movement or flipping.
What kind of bacteria motility technique uses agar medium with tritetrazilolum chloride?
What kind of bacteria motility technique uses agar medium with tritetrazilolum chloride?
What is a colorless dye used in the motility test agar medium?
What is a colorless dye used in the motility test agar medium?
How many hours do you incubate bacteria in the motility test agar?
How many hours do you incubate bacteria in the motility test agar?
Which technique is usually used for pathogenic bacteria?
Which technique is usually used for pathogenic bacteria?
Which two organisms were used for the experiment (not prepared slides)?
Which two organisms were used for the experiment (not prepared slides)?
Which step (of 4) in the Hanging Drop technique includes applying petroleum jelly along edges of the coverslip?
Which step (of 4) in the Hanging Drop technique includes applying petroleum jelly along edges of the coverslip?
Which step (of 4) in the Hanging Drop technique involves placing a loopful of culture with an inoculating loop in the center of the coverslip?
Which step (of 4) in the Hanging Drop technique involves placing a loopful of culture with an inoculating loop in the center of the coverslip?
Which step (of 4) in the Hanging Drop technique involves using a depression slide to place the concave area over the coverslip?
Which step (of 4) in the Hanging Drop technique involves using a depression slide to place the concave area over the coverslip?
Which step (of 4) in the Hanging Drop technique involves inverting the slide so the coverslip faces the nosepiece?
Which step (of 4) in the Hanging Drop technique involves inverting the slide so the coverslip faces the nosepiece?
Which step (of 3) in the Motility test medium involves suspending bacteria cultures by rolling the tube 20 times between your palms?
Which step (of 3) in the Motility test medium involves suspending bacteria cultures by rolling the tube 20 times between your palms?
Which step (of 3) in the Motility test medium involves incinerating the inoculating needle and dipping it into the culture?
Which step (of 3) in the Motility test medium involves incinerating the inoculating needle and dipping it into the culture?
Which step (of 3) in the Motility test medium involves incubating culture tubes for 24-48 hours?
Which step (of 3) in the Motility test medium involves incubating culture tubes for 24-48 hours?
What is the arrangement of the flagella for Proteus vulgaris?
What is the arrangement of the flagella for Proteus vulgaris?
What is the arrangement of the flagella for Spirillum volutans?
What is the arrangement of the flagella for Spirillum volutans?
Is Escherichia coli motile?
Is Escherichia coli motile?
Is Staphylococcus aureus motile?
Is Staphylococcus aureus motile?
What are two other methods of bacterial motility?
What are two other methods of bacterial motility?
How do flagella contribute to pathogenicity?
How do flagella contribute to pathogenicity?
What are two advantages of the Hanging Drop technique?
What are two advantages of the Hanging Drop technique?
What is an advantage of the motility test medium method?
What is an advantage of the motility test medium method?
Bacteria demonstrate motility in the motility medium by:
Bacteria demonstrate motility in the motility medium by:
Bacteria demonstrate non-motility in the motility medium by:
Bacteria demonstrate non-motility in the motility medium by:
Which tube demonstrates motility?
Which tube demonstrates motility?
Which tube demonstrates non-motility?
Which tube demonstrates non-motility?
What kind of bacteria is 'Atrichous'?
What kind of bacteria is 'Atrichous'?
What kind of bacteria is 'Monotrichous'?
What kind of bacteria is 'Monotrichous'?
What kind of bacteria is 'Peritrichous'?
What kind of bacteria is 'Peritrichous'?
What kind of bacteria is 'Lophotrichous'?
What kind of bacteria is 'Lophotrichous'?
What kind of bacteria is 'Amphitrichous'?
What kind of bacteria is 'Amphitrichous'?
Study Notes
Bacterial Flagella and Their Functions
- Bacterial flagella are thin, hair-like structures that enable motility in bacteria.
- Flagella serve the primary function of locomotion, allowing bacteria to move towards nutrients and evade harmful substances.
- Not all microorganisms possess flagella; their presence aids in identification and classification.
Types of Flagellar Distribution
- Atrichous: Bacteria without flagella.
- Monotrichous: A single flagellum at one pole of the bacterium.
- Lophotrichous: Several flagella originating from one pole.
- Amphitrichous: Flagella located at both poles of the bacterium.
- Peritrichous: Flagella distributed all around the bacterium.
Motility Testing Techniques
- Hanging Drop Technique: Utilizes a depression slide and allows for direct observation of motility under a microscope, including Brownian movement (vibration of non-motile bacteria).
- Motility Test Medium: Uses tritetrazilolum chloride dye; bacteria reduce the dye, causing a color change, aiding in observation of growth and motility patterns.
Observations and Results
- Motile bacteria exhibit directional movement, while non-motile bacteria appear to vibrate within their confined space, indicative of Brownian movement.
- Growth patterns in motility test medium indicate motility:
- Diffusion of growth away from the stab line indicates motile bacteria.
- Growth restricted to the stab line denotes non-motility.
Experimental Procedures
-
Steps for Hanging Drop Technique:
- Apply petroleum jelly to the coverslip edges.
- Place a loopful of bacterial culture at the center of the coverslip.
- Use a depression slide to secure the coverslip for observation.
- Invert the slide for microscopy.
-
Steps for Motility Test Medium:
- Roll culture tubes to suspend bacteria.
- Stab the medium with an inoculating needle.
- Incubate the tubes to observe growth patterns.
Examples of Bacterial Organisms
- Escherichia coli: Known to be motile.
- Staphylococcus aureus: Non-motile organism commonly used as a control.
Advantages of Motility Techniques
-
Hanging Drop Technique:
- Requires no heat treatment, preserving bacteria morphology.
- Provides immediate observation without incubation.
-
Motility Test Medium:
- Good for testing pathogenic organisms in controlled conditions.
Summary of Key Concepts
- Flagella contribute to pathogenicity by facilitating adhesion and invasion of host cells.
- Recognize staining methods to visualize flagella for identification purposes.
- Distinguish motility patterns through observation of growth diffusion in motility testing.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Explore the fascinating world of bacterial flagella and their critical role in motility. This quiz delves into various types of flagellar distributions and techniques used for testing bacterial motility. Test your understanding of these essential microbiological concepts!
