Applied Microbial Technology Lec 7: Ideal Production Microorganism Acquisition
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Questions and Answers

What should the construction of an ideal industrial production microorganism be based on?

  • Physical characteristics and growth rate
  • Metabolic pathways, regulatory mechanisms, and genetic basis (correct)
  • Environmental factors and external stimuli
  • Reproductive patterns and life cycle

Which method is used for the acquisition of new strains based on reason and logic?

  • Direct visualization of the most producing strains
  • Gene manipulations such as cell or protoplast fusion (correct)
  • Mutation-selection strain improvement
  • Microbial screening utilizing selection of the desired characteristic

What technique results in an increase in the number of a given organism relative to the numbers of other types in the original inoculum?

  • Gene manipulations
  • Enrichment culture (correct)
  • Direct visualization
  • Mutation-selection

In the context of microbial screening, what are isolation methods used to select for?

<p>Microbes exhibiting desirable characteristics (D)</p> Signup and view all the answers

What is the main focus of a general schematic of an industrial screening program?

<p>Selection of the desired characteristic (D)</p> Signup and view all the answers

What is the main reason for the low productivity of biologically active molecules synthesized by microorganisms isolated from nature?

<p>The molecules are nonessential for the survival of the microorganisms (B)</p> Signup and view all the answers

What is the most commonly sought phenotype in microbial strain improvement?

<p>Increased metabolite production (C)</p> Signup and view all the answers

What is one significant advantage of targeted approaches for developing improved microbial strains?

<p>Only a small number of strains have to be screened and evaluated (B)</p> Signup and view all the answers

What does the classical process of obtaining improved microbial strains involve?

<p>Random screening and assessment of mutagenesis survivors (A)</p> Signup and view all the answers

What is the most comprehensive knowledge that should be considered for the construction of an ideal industrial production microorganism?

<p>Metabolic pathways, regulatory mechanisms, and genetic basis (D)</p> Signup and view all the answers

Which method is used for the acquisition of new strains based on reason and logic?

<p>Rational enrichment techniques (B)</p> Signup and view all the answers

What is the primary purpose of the enrichment culture technique in microbial screening?

<p>To isolate and increase the number of desired microbes (A)</p> Signup and view all the answers

Which gene manipulation technique involves the fusion of cells or protoplasts to create new strains?

<p>In vitro DNA recombination (A)</p> Signup and view all the answers

What is the fundamental strategy behind the isolation methods used in microbial screening?

<p>To utilize selection for desired characteristics (B)</p> Signup and view all the answers

What is the primary purpose of microbial strain improvement?

<p>To enhance the production of biologically active molecules by microorganisms (C)</p> Signup and view all the answers

What is the key advantage of targeted approaches for developing improved microbial strains?

<p>They require screening and evaluation of only a small number of strains (B)</p> Signup and view all the answers

What characterizes the classical approach to microbial strain improvement?

<p>It entails random mutation and screening as the primary method for strain improvement (D)</p> Signup and view all the answers

What is the primary reason for the low productivity of biologically active molecules synthesized by microorganisms isolated from nature?

<p>The molecules being nonessential for the survival of the microorganisms (C)</p> Signup and view all the answers

What is a common strategy used to obtain improved microbial strains?

<p>Application of mutagens to increase genetic variability (B)</p> Signup and view all the answers

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