Apoptotic DNA Fragmentation Quiz
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Questions and Answers

What is the role of CAD in DNA fragmentation during apoptosis?

  • CAD inhibits the endonuclease activity
  • CAD prevents the cleavage of iCAD
  • CAD cuts the chromosomal DNA between nucleosomes (correct)
  • CAD promotes the formation of apoptotic nuclei

    • What is the consequence of cleavage of iCAD?

  • Inhibition of DNA fragmentation
  • Activation of executioner caspases
  • Unleashing of the nuclease CAD (correct)
  • Formation of apoptotic nuclei

    • How are DNA fragments separated by size during gel electrophoresis?

  • Based on their mobility in the gel (correct)
  • Based on their nucleotide sequence
  • Based on their association with iCAD
  • Based on their fluorescence intensity

    • What is the purpose of adding a fluorescent label to DNA ends in the mentioned technique?

    <p>To detect apoptotic nuclei</p> Signup and view all the answers

    What happens to the fragments during gel electrophoresis when cleavages occur in the linker regions between nucleosomes?

    <p>They separate into a characteristic ladder pattern</p> Signup and view all the answers

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