18 Questions
What is the purpose of using HAT Medium in antibody-producing cell selection?
To inhibit DNA synthesis in antibody-producing cells
Why is a single cell cultured in different tubes during the antibody production process?
To increase the chances of finding a monoclonal antibody-producing cell
What is the role of thymidine kinase (TK) in the HAT Medium-mediated cell selection process?
It phosphorylates thymidine absorbed by the cells into TMP
What is the consequence of blocking dihydrofolate reductase by aminopterin in the HAT Medium?
Prevention of tetrahydrofolate (THF) production
Why are positive clones selected during the antibody production process?
To ensure specificity for the same antigen
How does the HAT Medium help in maintaining monoclonal antibody-producing cells?
By blocking non-specific cell growth
What is one way to achieve even greater variation in Fv molecules?
Performing random mutagenesis of DNAs in the combinatorial library
Why were bacteriophage λ vectors engineered to insert the H and L chain DNA sequences into a site flanked by plasmid DNA sequences?
To facilitate the production of large numbers of Fv fragments in E.coli
What is a key advantage of phage display technology compared to traditional methods like animal immunization and hybridoma development?
No need for immunization
How can Fv fragments be produced in large quantities using the bacteriophage λ vector system?
By transforming plasmid DNA into E.coli
What technique can be used to increase the quantification of gene copy numbers in phage display?
Changing binding conditions
Why is it possible to replace animal immunization and hybridoma development with a bacterial system for antibody production?
Bacterial systems can produce unlimited quantities of antibodies
What is the purpose of the XenoMouse technology?
To integrate human immunoglobulin loci into a mouse chromosome
What causes replicative senescence in normal diploid cells?
Telomere shortening
What is the phenomenon known as the Hayflick limit?
Replicative senescence after a certain number of cell divisions
What is telomerase and its role in cell immortalization?
A reverse transcriptase enzyme
How does an immortalized cell line differ from a normal cell line?
Immortalized cell lines have evaded normal cellular senescence barriers
What triggers the phenomenon of replicative senescence in cells?
Telomere shortening
Test your knowledge on the construction and screening of antibody libraries, including shuffling L and H chains, random mutagenesis, and bacteriophage plaque screening. Learn about the technologies used to develop Fv molecules that recognize unusual epitopes.
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