Podcast
Questions and Answers
What is the purpose of using HAT Medium in antibody-producing cell selection?
What is the purpose of using HAT Medium in antibody-producing cell selection?
- To induce apoptosis in antibody-producing cells
- To enhance the mutation rate in antibody-producing cells
- To inhibit DNA synthesis in antibody-producing cells (correct)
- To promote the growth of antibody-producing cells
Why is a single cell cultured in different tubes during the antibody production process?
Why is a single cell cultured in different tubes during the antibody production process?
- To induce programmed cell death in the cultured cells
- To introduce genetic mutations in the cultured cells
- To increase the chances of finding a monoclonal antibody-producing cell (correct)
- To decrease the antibody production efficiency
What is the role of thymidine kinase (TK) in the HAT Medium-mediated cell selection process?
What is the role of thymidine kinase (TK) in the HAT Medium-mediated cell selection process?
- It enhances DNA replication in antibody-producing cells
- It phosphorylates thymidine absorbed by the cells into TMP (correct)
- It inhibits cloning of antibody-producing cells
- It promotes apoptosis in antibody-producing cells
What is the consequence of blocking dihydrofolate reductase by aminopterin in the HAT Medium?
What is the consequence of blocking dihydrofolate reductase by aminopterin in the HAT Medium?
Why are positive clones selected during the antibody production process?
Why are positive clones selected during the antibody production process?
How does the HAT Medium help in maintaining monoclonal antibody-producing cells?
How does the HAT Medium help in maintaining monoclonal antibody-producing cells?
What is one way to achieve even greater variation in Fv molecules?
What is one way to achieve even greater variation in Fv molecules?
Why were bacteriophage λ vectors engineered to insert the H and L chain DNA sequences into a site flanked by plasmid DNA sequences?
Why were bacteriophage λ vectors engineered to insert the H and L chain DNA sequences into a site flanked by plasmid DNA sequences?
What is a key advantage of phage display technology compared to traditional methods like animal immunization and hybridoma development?
What is a key advantage of phage display technology compared to traditional methods like animal immunization and hybridoma development?
How can Fv fragments be produced in large quantities using the bacteriophage λ vector system?
How can Fv fragments be produced in large quantities using the bacteriophage λ vector system?
What technique can be used to increase the quantification of gene copy numbers in phage display?
What technique can be used to increase the quantification of gene copy numbers in phage display?
Why is it possible to replace animal immunization and hybridoma development with a bacterial system for antibody production?
Why is it possible to replace animal immunization and hybridoma development with a bacterial system for antibody production?
What is the purpose of the XenoMouse technology?
What is the purpose of the XenoMouse technology?
What causes replicative senescence in normal diploid cells?
What causes replicative senescence in normal diploid cells?
What is the phenomenon known as the Hayflick limit?
What is the phenomenon known as the Hayflick limit?
What is telomerase and its role in cell immortalization?
What is telomerase and its role in cell immortalization?
How does an immortalized cell line differ from a normal cell line?
How does an immortalized cell line differ from a normal cell line?
What triggers the phenomenon of replicative senescence in cells?
What triggers the phenomenon of replicative senescence in cells?
