Antibody Library Construction and Screening Quiz

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What is the purpose of using HAT Medium in antibody-producing cell selection?

To induce apoptosis in antibody-producing cells

To enhance the mutation rate in antibody-producing cells

To inhibit DNA synthesis in antibody-producing cells (correct)

To promote the growth of antibody-producing cells

Why is a single cell cultured in different tubes during the antibody production process?

To induce programmed cell death in the cultured cells

To introduce genetic mutations in the cultured cells

To increase the chances of finding a monoclonal antibody-producing cell (correct)

To decrease the antibody production efficiency

What is the role of thymidine kinase (TK) in the HAT Medium-mediated cell selection process?

It enhances DNA replication in antibody-producing cells

It phosphorylates thymidine absorbed by the cells into TMP (correct)

It inhibits cloning of antibody-producing cells

It promotes apoptosis in antibody-producing cells

What is the consequence of blocking dihydrofolate reductase by aminopterin in the HAT Medium?

Prevention of tetrahydrofolate (THF) production (B) Signup and view all the answers

Why are positive clones selected during the antibody production process?

To ensure specificity for the same antigen (B) Signup and view all the answers

How does the HAT Medium help in maintaining monoclonal antibody-producing cells?

By blocking non-specific cell growth (B) Signup and view all the answers

What is one way to achieve even greater variation in Fv molecules?

Performing random mutagenesis of DNAs in the combinatorial library (A) Signup and view all the answers

Why were bacteriophage λ vectors engineered to insert the H and L chain DNA sequences into a site flanked by plasmid DNA sequences?

To facilitate the production of large numbers of Fv fragments in E.coli (B) Signup and view all the answers

What is a key advantage of phage display technology compared to traditional methods like animal immunization and hybridoma development?

No need for immunization (C) Signup and view all the answers

How can Fv fragments be produced in large quantities using the bacteriophage λ vector system?

By transforming plasmid DNA into E.coli (D) Signup and view all the answers

What technique can be used to increase the quantification of gene copy numbers in phage display?

Changing binding conditions (B) Signup and view all the answers

Why is it possible to replace animal immunization and hybridoma development with a bacterial system for antibody production?

Bacterial systems can produce unlimited quantities of antibodies (B) Signup and view all the answers

What is the purpose of the XenoMouse technology?

To integrate human immunoglobulin loci into a mouse chromosome (B) Signup and view all the answers

What causes replicative senescence in normal diploid cells?

Telomere shortening (A) Signup and view all the answers

What is the phenomenon known as the Hayflick limit?

Replicative senescence after a certain number of cell divisions (D) Signup and view all the answers

What is telomerase and its role in cell immortalization?

A reverse transcriptase enzyme (A) Signup and view all the answers

How does an immortalized cell line differ from a normal cell line?

Immortalized cell lines have evaded normal cellular senescence barriers (A) Signup and view all the answers

What triggers the phenomenon of replicative senescence in cells?

Telomere shortening (B) Signup and view all the answers

Antibody Library Construction and Screening Quiz

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Questions and Answers

What is the purpose of using HAT Medium in antibody-producing cell selection?

Why is a single cell cultured in different tubes during the antibody production process?

What is the role of thymidine kinase (TK) in the HAT Medium-mediated cell selection process?

What is the consequence of blocking dihydrofolate reductase by aminopterin in the HAT Medium?

Why are positive clones selected during the antibody production process?

How does the HAT Medium help in maintaining monoclonal antibody-producing cells?

What is one way to achieve even greater variation in Fv molecules?

Why were bacteriophage λ vectors engineered to insert the H and L chain DNA sequences into a site flanked by plasmid DNA sequences?

What is a key advantage of phage display technology compared to traditional methods like animal immunization and hybridoma development?

How can Fv fragments be produced in large quantities using the bacteriophage λ vector system?

What technique can be used to increase the quantification of gene copy numbers in phage display?

Why is it possible to replace animal immunization and hybridoma development with a bacterial system for antibody production?

What is the purpose of the XenoMouse technology?

What causes replicative senescence in normal diploid cells?

What is the phenomenon known as the Hayflick limit?

What is telomerase and its role in cell immortalization?

How does an immortalized cell line differ from a normal cell line?

What triggers the phenomenon of replicative senescence in cells?

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