Podcast
Questions and Answers
Which type of antibodies are typically responsible for causing decreased survival of RBCs possessing the target antigen?
Which type of antibodies are typically responsible for causing decreased survival of RBCs possessing the target antigen?
- IgA antibodies
- IgM antibodies
- IgG antibodies (correct)
- IgE antibodies
At what temperature do clinically significant alloantibodies typically react?
At what temperature do clinically significant alloantibodies typically react?
- 37°C (correct)
- 50°C
- 25°C
- 0°C
In which phase of the indirect antiglobulin test (IAT) do clinically significant alloantibodies typically react?
In which phase of the indirect antiglobulin test (IAT) do clinically significant alloantibodies typically react?
- Phase 3
- Phase 4
- Phase 2 (correct)
- Phase 1
What is performed to determine the specificity of an unexpected antibody detected in the antibody screen?
What is performed to determine the specificity of an unexpected antibody detected in the antibody screen?
What can be ascertained once the specificity of an antibody is identified?
What can be ascertained once the specificity of an antibody is identified?
Match the following types of antibodies with their descriptions:
Match the following types of antibodies with their descriptions:
Which population group requires antibody screen testing as part of pretransfusion compatibility testing?
Which population group requires antibody screen testing as part of pretransfusion compatibility testing?
What is the purpose of including an antibody screen in standard prenatal testing for obstetric patients?
What is the purpose of including an antibody screen in standard prenatal testing for obstetric patients?
In what situations may an antibody screen be performed when evaluating the compatibility of allogeneic hematopoietic progenitor cell (HPC) and bone marrow donors with the intended transplant recipient?
In what situations may an antibody screen be performed when evaluating the compatibility of allogeneic hematopoietic progenitor cell (HPC) and bone marrow donors with the intended transplant recipient?
Which method is used to detect clinically significant antibodies in a test tube?
Which method is used to detect clinically significant antibodies in a test tube?
What is the purpose of the immediate spin (IS) phase in the test tube method?
What is the purpose of the immediate spin (IS) phase in the test tube method?
What is the purpose of the 37°C incubation phase in the test tube method?
What is the purpose of the 37°C incubation phase in the test tube method?
What does the addition of enhancement reagent in the test tube method do?
What does the addition of enhancement reagent in the test tube method do?
When is observation for hemolysis required in the test tube method?
When is observation for hemolysis required in the test tube method?
How is agglutination observed in the test tube method?
How is agglutination observed in the test tube method?
What is the purpose of the AHG reagent in the test tube method?
What is the purpose of the AHG reagent in the test tube method?
What is the concentration range of RBC reagents used in the test tube method?
What is the concentration range of RBC reagents used in the test tube method?
What is the purpose of the IAT in the test tube method?
What is the purpose of the IAT in the test tube method?
What is the purpose of the centrifugation step in the test tube method?
What is the purpose of the centrifugation step in the test tube method?
Which type of AHG reagent is not often used when performing antibody detection testing?
Which type of AHG reagent is not often used when performing antibody detection testing?
Which type of AHG reagent is also called polyvalent or broad spectrum Coombs’ serum?
Which type of AHG reagent is also called polyvalent or broad spectrum Coombs’ serum?
Which type of AHG reagent contains antibodies to both IgG and complement components?
Which type of AHG reagent contains antibodies to both IgG and complement components?
What is used to control a negative antiglobulin test after adding the AHG reagent?
What is used to control a negative antiglobulin test after adding the AHG reagent?
What does the addition of Coombs’ control cells in a negative antiglobulin test prove?
What does the addition of Coombs’ control cells in a negative antiglobulin test prove?
What should be done if the Coombs’ control cells fail to agglutinate in a negative antiglobulin test?
What should be done if the Coombs’ control cells fail to agglutinate in a negative antiglobulin test?
What is the purpose of the antibody screen in standard prenatal testing for obstetric patients?
What is the purpose of the antibody screen in standard prenatal testing for obstetric patients?
What should be done if a tube test is negative after adding the AHG reagent?
What should be done if a tube test is negative after adding the AHG reagent?
What is the purpose of using group O cells in antibody screen testing?
What is the purpose of using group O cells in antibody screen testing?
What should be observed for when using a pooled reagent for blood donor antibody screening?
What should be observed for when using a pooled reagent for blood donor antibody screening?
Which antigens should be expressed in the reagent set or pooled reagent used for antibody screen testing?
Which antigens should be expressed in the reagent set or pooled reagent used for antibody screen testing?
What is the significance of antibodies that show dosage in antibody screening?
What is the significance of antibodies that show dosage in antibody screening?
Which antibodies may show dosage and react more strongly with cells having homozygous antigen expression?
Which antibodies may show dosage and react more strongly with cells having homozygous antigen expression?
Which type of AHG reagent is also known as polyvalent or broad spectrum Coombs’ serum?
Which type of AHG reagent is also known as polyvalent or broad spectrum Coombs’ serum?
