Podcast
Questions and Answers
What is the primary effect of the CNCL1 gene on muscle function?
What is the primary effect of the CNCL1 gene on muscle function?
- It causes muscle contractions due to increased potassium levels.
- It enhances chloride removal, preventing muscle fatigue.
- It inhibits the chloride pump, causing muscle 'freeze'. (correct)
- It directly promotes muscle growth and strength.
Which of the following poses a primary challenge to laying hens when transitioning from a closed system to free-range?
Which of the following poses a primary challenge to laying hens when transitioning from a closed system to free-range?
- Decreased exposure to natural light causing Vitamin D deficiency.
- Reduced environmental stimulation leading to boredom.
- Increased access to high quality feed, without limitations.
- Increased risk of exposure to a wider variety of pathogens. (correct)
What does 'epitype' refer to in the context of matching animals to their environment?
What does 'epitype' refer to in the context of matching animals to their environment?
- The physical characteristics determined by genetics and environment. (correct)
- The genetic makeup of the animal.
- The behavioral adaptations of the animal over time.
- The specific diet requirements of a breed of animal.
Why are many farmed species not well matched to intensive farming systems?
Why are many farmed species not well matched to intensive farming systems?
What is a significant limitation of using flock/herd mortality as a stress indicator?
What is a significant limitation of using flock/herd mortality as a stress indicator?
Which of the following is considered the best indicator of real-time stress monitoring in animals according to the passage?
Which of the following is considered the best indicator of real-time stress monitoring in animals according to the passage?
Why is it advantageous for a stress biomarker to be easily obtainable?
Why is it advantageous for a stress biomarker to be easily obtainable?
A measurable substance in an organism whose presence indicates a disease or environmental exposure is called a:
A measurable substance in an organism whose presence indicates a disease or environmental exposure is called a:
What is the primary purpose of using a novel antigen (Ag) or a vaccine to induce AbMIR?
What is the primary purpose of using a novel antigen (Ag) or a vaccine to induce AbMIR?
How does the level of antibody production change with subsequent exposures to the same antigen?
How does the level of antibody production change with subsequent exposures to the same antigen?
What was the specific stressor used in the immune challenge experiment with sheep, and why was it chosen?
What was the specific stressor used in the immune challenge experiment with sheep, and why was it chosen?
In the sheep experiment, what was the significance of some sheep not responding to the bacterial endotoxin?
In the sheep experiment, what was the significance of some sheep not responding to the bacterial endotoxin?
According to the content, what is a key difference observed in the immune response to acute versus chronic stress?
According to the content, what is a key difference observed in the immune response to acute versus chronic stress?
What role was cortisol playing when endotoxins were injected in sheep during the experiment?
What role was cortisol playing when endotoxins were injected in sheep during the experiment?
Which of the following best describes the effect of glucocorticoids on the immune system?
Which of the following best describes the effect of glucocorticoids on the immune system?
What was the main conclusion regarding individual response to stress from the study?
What was the main conclusion regarding individual response to stress from the study?
What is a potential consequence of an animal's inability to recover from stress or habituate to a stressor?
What is a potential consequence of an animal's inability to recover from stress or habituate to a stressor?
During early development, which system is particularly sensitive to epigenetic modifications which highlights the importance of the microbiome?
During early development, which system is particularly sensitive to epigenetic modifications which highlights the importance of the microbiome?
How might single-nucleotide polymorphisms (SNPs) in cytochrome p450 (CYP) enzymes affect glucocorticoid levels?
How might single-nucleotide polymorphisms (SNPs) in cytochrome p450 (CYP) enzymes affect glucocorticoid levels?
Which of the following is an example of antimicrobial misuse in animal production?
Which of the following is an example of antimicrobial misuse in animal production?
In what way could antimicrobial resistance (AMR) be disseminated within an agricultural environment?
In what way could antimicrobial resistance (AMR) be disseminated within an agricultural environment?
Which of the following best describes the role of MHC-I molecules?
Which of the following best describes the role of MHC-I molecules?
What is the primary function of cytokines like IL-4 and IL-13 released by T helper cells?
What is the primary function of cytokines like IL-4 and IL-13 released by T helper cells?
How does the acquired immune response differ from the innate immune response?
How does the acquired immune response differ from the innate immune response?
Why are antibodies considered good biomarkers for infection?
Why are antibodies considered good biomarkers for infection?
What is a key difference between bovine IgG1 and IgG2 in blood?
What is a key difference between bovine IgG1 and IgG2 in blood?
Which of the following is NOT a known alarmins or DAMPs (distress signal) producer?
Which of the following is NOT a known alarmins or DAMPs (distress signal) producer?
What is the role of cytotoxic T cells in the cell-mediated immune response?
What is the role of cytotoxic T cells in the cell-mediated immune response?
What is a limitation of using cytokines as a biomarker for stress?
What is a limitation of using cytokines as a biomarker for stress?
Why is it important to consider sampling location when measuring antibody isotypes?
Why is it important to consider sampling location when measuring antibody isotypes?
Which T cell helps in generating both antibody-mediated (AbMIR) and cell-mediated (CMIR) immune responses?
Which T cell helps in generating both antibody-mediated (AbMIR) and cell-mediated (CMIR) immune responses?
What is the primary mechanism by which dexamethasone treats autoimmune skin conditions?
What is the primary mechanism by which dexamethasone treats autoimmune skin conditions?
Why is early diagnosis of Johne's Disease in cattle challenging?
Why is early diagnosis of Johne's Disease in cattle challenging?
Which of the following biomarkers is most effective for monitoring stages 1-3d of Johne's Disease?
Which of the following biomarkers is most effective for monitoring stages 1-3d of Johne's Disease?
What can be assessed by monitoring SAM, HPA, AbMIR, and CMIR following an immune challenge?
What can be assessed by monitoring SAM, HPA, AbMIR, and CMIR following an immune challenge?
Which of these is an example of a biological stressor that could be used in a stress challenge?
Which of these is an example of a biological stressor that could be used in a stress challenge?
How do epigenetic variants differ from genetic variants?
How do epigenetic variants differ from genetic variants?
What is the typical effect of cytosine methylation on gene expression?
What is the typical effect of cytosine methylation on gene expression?
How do glucocorticoids (GCs) impact CMIR in the context of stress?
How do glucocorticoids (GCs) impact CMIR in the context of stress?
Which of the following is a direct consequence of Addison's disease?
Which of the following is a direct consequence of Addison's disease?
What is a typical diagnostic symptom observed in individuals with Addison's disease?
What is a typical diagnostic symptom observed in individuals with Addison's disease?
What is a primary limitation of using ACTH as a biomarker for stress?
What is a primary limitation of using ACTH as a biomarker for stress?
Why is the 'white coat effect' useful in research?
Why is the 'white coat effect' useful in research?
Which of the following best describes why 'Chromogranin A' is considered an indirect biomarker of stress?
Which of the following best describes why 'Chromogranin A' is considered an indirect biomarker of stress?
Why is measuring glucocorticoid levels over a stress challenge, rather than at a single time point, advantageous?
Why is measuring glucocorticoid levels over a stress challenge, rather than at a single time point, advantageous?
Which of the following correctly describes how commensal gut bacteria contribute to host defense?
Which of the following correctly describes how commensal gut bacteria contribute to host defense?
What role do cytokines serve in the acute-phase response during an infection?
What role do cytokines serve in the acute-phase response during an infection?
What is the significance of 'alarmins' in the context of innate immunity?
What is the significance of 'alarmins' in the context of innate immunity?
How does antibody-mediated immunity (AbMIR) primarily target pathogens?
How does antibody-mediated immunity (AbMIR) primarily target pathogens?
Why is tolerance to commensal microbes essential for survival?
Why is tolerance to commensal microbes essential for survival?
Which of the following parameters is least likely to influence the measurement of 'Chromogranin A' levels?
Which of the following parameters is least likely to influence the measurement of 'Chromogranin A' levels?
How do non-immune barriers such as skin and mucous membranes protect the body from microbial growth?
How do non-immune barriers such as skin and mucous membranes protect the body from microbial growth?
What is a 'cytokine storm', and why is it dangerous?
What is a 'cytokine storm', and why is it dangerous?
What is the primary strategy for improving host defense against pathogens?
What is the primary strategy for improving host defense against pathogens?
What is the main characteristic of the innate immune response that differentiates it from the acquired immune response?
What is the main characteristic of the innate immune response that differentiates it from the acquired immune response?
Which of the following is a characteristic of glucocorticoids that must be considered when using them as a stress biomarker?
Which of the following is a characteristic of glucocorticoids that must be considered when using them as a stress biomarker?
Flashcards
CNCL1 gene
CNCL1 gene
A gene that influences the function of a chloride channel protein, leading to muscle freezing or immobility due to chloride accumulation.
Biomarker
Biomarker
A measurable substance in an organism that indicates the presence of a condition, disease, or environmental exposure.
Systemic Biomarker
Systemic Biomarker
A biomarker of stress that reflects a systemic response, affecting the entire body.
Local Biomarker
Local Biomarker
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Somatic Cell Count (SCC)
Somatic Cell Count (SCC)
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Mastitis
Mastitis
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Learned Helplessness
Learned Helplessness
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Real-time Behaviour Monitoring
Real-time Behaviour Monitoring
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Immune Response Capacity
Immune Response Capacity
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Antibody Levels
Antibody Levels
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Immune Efficacy
Immune Efficacy
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Immune Challenge
Immune Challenge
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Bacterial Endotoxin Challenge
Bacterial Endotoxin Challenge
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Cortisol
Cortisol
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Individual Differences in Stress Response
Individual Differences in Stress Response
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Chronic Stress and Immunosuppression
Chronic Stress and Immunosuppression
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Cytokines
Cytokines
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Innate Immunity
Innate Immunity
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Acquired Immunity
Acquired Immunity
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Phagocytes
Phagocytes
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Antibodies
Antibodies
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Major Histocompatibility Complex (MHC)
Major Histocompatibility Complex (MHC)
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T Helper Cells
T Helper Cells
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Cytotoxic T Cells
Cytotoxic T Cells
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Dendritic Cells
Dendritic Cells
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Antibody-Mediated Immune Response (AbMIR)
Antibody-Mediated Immune Response (AbMIR)
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ACTH (Adrenocorticotropic Hormone)
ACTH (Adrenocorticotropic Hormone)
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Norepinephrine (NE)
Norepinephrine (NE)
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Epinephrine (E)
Epinephrine (E)
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Chromogranin A
Chromogranin A
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Immunoregulation
Immunoregulation
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Antigen (Ag)
Antigen (Ag)
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Antibody (Ab)
Antibody (Ab)
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Pro-inflammatory Cytokine
Pro-inflammatory Cytokine
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Systemic Inflammatory Response Syndrome (SIRS)
Systemic Inflammatory Response Syndrome (SIRS)
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Acute-phase Response
Acute-phase Response
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Acquired Immune Response (AIR)
Acquired Immune Response (AIR)
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Innate Immune Response (IIR)
Innate Immune Response (IIR)
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Panel of Biomarkers
Panel of Biomarkers
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Addison's Disease
Addison's Disease
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Stress Resilience
Stress Resilience
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Cushing's Disease
Cushing's Disease
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Epigenetic Changes
Epigenetic Changes
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MicroRNA (miRNA)
MicroRNA (miRNA)
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DNA Methylation
DNA Methylation
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Histone Modification
Histone Modification
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Epigenetics
Epigenetics
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Genes Involved in Stress Response
Genes Involved in Stress Response
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Neuroendocrine Immune System
Neuroendocrine Immune System
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Antimicrobial Usage in Animal Production
Antimicrobial Usage in Animal Production
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Study Notes
Stress and Animal Health
- Stress negatively impacts animal production, product quality, and welfare.
- Consumers expect high quality, ethically raised animals, often at lower prices.
- Zoonotic diseases (diseases that transfer from animals to humans) are a concern.
- Antimicrobial resistance is a growing problem in animal health, affecting both human and animal effectiveness.
- Genetic selection for production traits can negatively correlate with health and fertility.
- Climate change is expected to affect livestock and aquaculture.
- Physiological effects of stress are important to study for stress resistant animals.
- Domestic animals face physical, chemical, biological, and psychological stressors.
Response to Stress
- Stress response is a conserved reaction in mammals.
- Stress responses include cardiovascular, metabolic, musculoskeletal, neuroendocrine, and immune changes.
- Stress response triggers might vary between species and individuals.
- Stressors disrupt homeostasis.
- Physical stressors include temperature, climate change, shipping, air and water quality, light intensity, injury, stray voltage, UV radiation, processing, noise, and restraint.
- Chemical stressors include mycotoxins, heavy metals, organochlorides, and poisonous plants.
- Biological stressors include nutrient excess/deficiency, feed restriction, parturition, negative energy balance, and infection.
- Psychological stressors include mixing, weaning, herding, and showing.
Microbiome
- Microbes on epithelial surfaces provide nutrients (e.g., Vitamin K).
- Competition for space and resources between pathogens and microbiome is important.
- Regulation of inflammation is affected by the aging process.
- Sensory inputs (neural, endocrine, immune) lead to stress responses.
- Variation in stress responses is due to genetics, environment, and gene-environment interactions.
- Chronic stress affects behavior and immune function due to glucocorticoids and mineralocorticoid receptors.
- Circadian rhythm impacts cortisol levels and stress pathways.
Stress Physiology
- Exotic species may not be well-matched to intensive farming systems.
- Physiological indicators of stress include mortality, weight loss, decreased fertility, and clinical disease.
- Behavioral changes are important for monitoring stress.
- Biomarkers can measure stress and disease in animals.
- SCC (somatic cell count) is useful to detect mastitis in dairy cows.
- Real-time monitoring improves the early detection and assessment of stress.
- Physiological pathways of stress include the SAM (Sympathetic-Adrenal Medulla) axis and the HPA (Hypothalamic-Pituitary-Adrenal) axis.
Stress Biochemistry
- Stress leads to ACh release into the bloodstream.
- Cortisol release is part of the HPA axis.
- Corticosteroids are involved in stress response.
- Mineralocorticoid receptors and glucocorticoid receptors are key in stress responses.
- Chronic stress can cause changes in behavior and immune function.
Stress Regulation and Other Considerations
- Immediate neural pathways versus neuroendocrine pathways for stress response.
- Circadian rhythm influences stress response and hormone levels.
- Chronic stress leads to desensitization of cortisol receptors.
- Stress and genetics interaction is important for stress resistance.
Immunoregulation
- Immune systems can be activated by infectious agents or stressors.
- Immune functions are dependent on several factors that influence activation and response.
- Microbial stressors may require a panel of biomarkers for evaluation.
- Innate and acquired immunity mechanisms play roles in stress response.
Host Defense
- Definitions relating to concepts such as antibodies, antigens, TNF, IL, PAMPs, and DAMPs are provided.
- Immune systems signal danger and trigger stress responses.
- Systemic inflammatory response syndrome (SIRS) relates to overly enthusiastic cytokine response during stress.
- Acute-phase response, or cytokine storm, leads to potentially fatal organ failure.
- Innate immunity provides the first line of response while acquired immunity provides a sustained response.
Antimicrobials
- Antimicrobial usage is prominent in animal production.
- Inappropriate antimicrobial use can contribute to antimicrobial resistance.
- Misuse of antimicrobials leads to negative effects in animals and humans.
Summary
- The topics cover the physiological response to stress in diverse animal species, which has significant influences and impact on the health, productivity, and welfare of the animal.
- It also highlights the interaction of genetics, environment, and other factors in determining stress response.
- Understanding these considerations is important for improving animal health and welfare.
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