Anatomy and Physiology Pocket Guide Review
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Questions and Answers

What did the Amazon reviewers mention about the anatomy book?

  • The pocket size is misleading (correct)
  • It is not useful for clinical studies
  • It is not detailed or complete
  • It has no positive reviews

    • Who is the author of the book mentioned in the text?

  • Lela Buckingham, PhD, MB (ASCP), DLM (ASCP) (correct)
  • A reviewer on Amazon
  • A professor at Rush University Medical Center
  • An unnamed student

    • What type of promotion is currently available for individual purchases from FADavis.com?

  • 50% discount with free shipping
  • 25% discount with free returns
  • Buy one get one free offer
  • 20% discount with free shipping (correct)

    • What is the ultimate goal of applying innovative technology to molecular laboratory science?

    <p>To improve patient care</p> Signup and view all the answers

    What did the Amazon reviewers say about the usefulness of the resource for clinical studies?

    <p>It has been helpful through clinical studies</p> Signup and view all the answers

    What did the Amazon reviewers stress about the anatomy guide despite its size?

    <p>The guide is very detailed and complete</p> Signup and view all the answers

    What is the function of primase in DNA synthesis?

    <p>Catalyzing the synthesis of RNA primers on the lagging strand</p> Signup and view all the answers

    In which direction are both strands of the double helix read during replication?

    <p>5' to 3' on both strands</p> Signup and view all the answers

    Which enzyme is responsible for synthesizing deoxyribose nucleotides to extend the DNA chain?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the role of DNA polymerase on the lagging strand?

    <p>Synthesizing DNA discontinuously in the 3' to 5' direction</p> Signup and view all the answers

    Which enzyme component provides a hydroxyl group required for DNA synthesis?

    <p>Primase</p> Signup and view all the answers

    What is the function of helicase during DNA replication?

    <p>Unwinding the double helix to expose the template strands</p> Signup and view all the answers

    What did Julius Adler, Sylvy Kornberg, and Steven B. Zimmerman show about the new DNA?

    <p>It was a copy of the input DNA with the same A-T to G-C bp ratio.</p> Signup and view all the answers

    What is one function that most DNA polymerases have besides polymerization?

    <p>Pyrophosphorolysis</p> Signup and view all the answers

    What is the purpose of the exonuclease function in DNA polymerases?

    <p>To protect the sequence of nucleotides during synthesis</p> Signup and view all the answers

    Which fragment of E.coli DNA pol I carries the exonuclease activity?

    <p>Klenow fragment</p> Signup and view all the answers

    What would happen if copying errors occur during DNA replication?

    <p>Base changes or mutations in the DNA would result</p> Signup and view all the answers

    Which direction can DNA polymerase enzymes synthesize DNA?

    <p>5′ to 3′ only</p> Signup and view all the answers

    Which type of DNA ends are not suitable for exonucleases to work on?

    <p>Closed circular DNA</p> Signup and view all the answers

    What substrate requirement affects the activity of exonuclease I from E.coli?

    <p>Single-stranded DNA</p> Signup and view all the answers

    Which type of DNA ends can Exonuclease III efficiently remove mononucleotides from?

    <p>Recessed ends</p> Signup and view all the answers

    What additional activity does Exo III have besides removing mononucleotides from DNA ends?

    <p>Cutting DNA at apurinic sites</p> Signup and view all the answers

    When using Exonuclease VII from E.coli, which type of DNA ends can be digested by this enzyme?

    <p>3′-hydroxyl ends</p> Signup and view all the answers

    For what purpose was Exo III used in the research setting according to the text?

    <p>Creating nested deletions</p> Signup and view all the answers

    What is a unique feature of Exo VII among enzymes?

    <p>5′ exonuclease activity</p> Signup and view all the answers

    How can Exo VII be utilized in DNA manipulation?

    <p>To remove long single strands from DNA</p> Signup and view all the answers

    What was a limitation of the initial method used for joining separate DNA helices?

    <p>It required the chance positioning of two adjacent ends</p> Signup and view all the answers

    How did H. Gobind Khorana demonstrate efficient joining of DNA fragments?

    <p>By employing short synthetic segments with single-strand complementary overhangs</p> Signup and view all the answers

    What was observed to increase the efficiency of joining the ends of DNA molecules from certain bacterial viruses?

    <p>Treating DNA ends with terminal transferase</p> Signup and view all the answers

    Why was hydrogen bonding between single strands considered important for efficient ligation?

    <p>To hold ends together before ligation</p> Signup and view all the answers

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