Anatomy and Physiology Pocket Guide Review

What did the Amazon reviewers mention about the anatomy book?

The pocket size is misleading

Who is the author of the book mentioned in the text?

Lela Buckingham, PhD, MB (ASCP), DLM (ASCP)

What type of promotion is currently available for individual purchases from FADavis.com?

20% discount with free shipping

What is the ultimate goal of applying innovative technology to molecular laboratory science?

To improve patient care Signup and view all the answers

What did the Amazon reviewers say about the usefulness of the resource for clinical studies?

It has been helpful through clinical studies Signup and view all the answers

What did the Amazon reviewers stress about the anatomy guide despite its size?

The guide is very detailed and complete Signup and view all the answers

What is the function of primase in DNA synthesis?

Catalyzing the synthesis of RNA primers on the lagging strand Signup and view all the answers

In which direction are both strands of the double helix read during replication?

5' to 3' on both strands Signup and view all the answers

Which enzyme is responsible for synthesizing deoxyribose nucleotides to extend the DNA chain?

DNA polymerase Signup and view all the answers

What is the role of DNA polymerase on the lagging strand?

Synthesizing DNA discontinuously in the 3' to 5' direction Signup and view all the answers

Which enzyme component provides a hydroxyl group required for DNA synthesis?

Primase Signup and view all the answers

What is the function of helicase during DNA replication?

Unwinding the double helix to expose the template strands Signup and view all the answers

What did Julius Adler, Sylvy Kornberg, and Steven B. Zimmerman show about the new DNA?

It was a copy of the input DNA with the same A-T to G-C bp ratio. Signup and view all the answers

What is one function that most DNA polymerases have besides polymerization?

Pyrophosphorolysis Signup and view all the answers

What is the purpose of the exonuclease function in DNA polymerases?

To protect the sequence of nucleotides during synthesis Signup and view all the answers

Which fragment of E.coli DNA pol I carries the exonuclease activity?

Klenow fragment Signup and view all the answers

What would happen if copying errors occur during DNA replication?

Base changes or mutations in the DNA would result Signup and view all the answers

Which direction can DNA polymerase enzymes synthesize DNA?

5′ to 3′ only Signup and view all the answers

Which type of DNA ends are not suitable for exonucleases to work on?

Closed circular DNA Signup and view all the answers

What substrate requirement affects the activity of exonuclease I from E.coli?

Single-stranded DNA Signup and view all the answers

Which type of DNA ends can Exonuclease III efficiently remove mononucleotides from?

Recessed ends Signup and view all the answers

What additional activity does Exo III have besides removing mononucleotides from DNA ends?

Cutting DNA at apurinic sites Signup and view all the answers

When using Exonuclease VII from E.coli, which type of DNA ends can be digested by this enzyme?

3′-hydroxyl ends Signup and view all the answers

For what purpose was Exo III used in the research setting according to the text?

Creating nested deletions Signup and view all the answers

What is a unique feature of Exo VII among enzymes?

5′ exonuclease activity Signup and view all the answers

How can Exo VII be utilized in DNA manipulation?

To remove long single strands from DNA Signup and view all the answers

What was a limitation of the initial method used for joining separate DNA helices?

It required the chance positioning of two adjacent ends Signup and view all the answers

How did H. Gobind Khorana demonstrate efficient joining of DNA fragments?

By employing short synthetic segments with single-strand complementary overhangs Signup and view all the answers

What was observed to increase the efficiency of joining the ends of DNA molecules from certain bacterial viruses?

Treating DNA ends with terminal transferase Signup and view all the answers

Why was hydrogen bonding between single strands considered important for efficient ligation?

To hold ends together before ligation Signup and view all the answers