Anatomy and Physiology Pocket Guide Review
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Questions and Answers

What did the Amazon reviewers mention about the anatomy book?

  • The pocket size is misleading (correct)
  • It is not useful for clinical studies
  • It is not detailed or complete
  • It has no positive reviews

Who is the author of the book mentioned in the text?

  • Lela Buckingham, PhD, MB (ASCP), DLM (ASCP) (correct)
  • A reviewer on Amazon
  • A professor at Rush University Medical Center
  • An unnamed student

What type of promotion is currently available for individual purchases from FADavis.com?

  • 50% discount with free shipping
  • 25% discount with free returns
  • Buy one get one free offer
  • 20% discount with free shipping (correct)

What is the ultimate goal of applying innovative technology to molecular laboratory science?

<p>To improve patient care (D)</p> Signup and view all the answers

What did the Amazon reviewers say about the usefulness of the resource for clinical studies?

<p>It has been helpful through clinical studies (C)</p> Signup and view all the answers

What did the Amazon reviewers stress about the anatomy guide despite its size?

<p>The guide is very detailed and complete (B)</p> Signup and view all the answers

What is the function of primase in DNA synthesis?

<p>Catalyzing the synthesis of RNA primers on the lagging strand (C)</p> Signup and view all the answers

In which direction are both strands of the double helix read during replication?

<p>5' to 3' on both strands (B)</p> Signup and view all the answers

Which enzyme is responsible for synthesizing deoxyribose nucleotides to extend the DNA chain?

<p>DNA polymerase (C)</p> Signup and view all the answers

What is the role of DNA polymerase on the lagging strand?

<p>Synthesizing DNA discontinuously in the 3' to 5' direction (D)</p> Signup and view all the answers

Which enzyme component provides a hydroxyl group required for DNA synthesis?

<p>Primase (D)</p> Signup and view all the answers

What is the function of helicase during DNA replication?

<p>Unwinding the double helix to expose the template strands (D)</p> Signup and view all the answers

What did Julius Adler, Sylvy Kornberg, and Steven B. Zimmerman show about the new DNA?

<p>It was a copy of the input DNA with the same A-T to G-C bp ratio. (A)</p> Signup and view all the answers

What is one function that most DNA polymerases have besides polymerization?

<p>Pyrophosphorolysis (D)</p> Signup and view all the answers

What is the purpose of the exonuclease function in DNA polymerases?

<p>To protect the sequence of nucleotides during synthesis (A)</p> Signup and view all the answers

Which fragment of E.coli DNA pol I carries the exonuclease activity?

<p>Klenow fragment (A)</p> Signup and view all the answers

What would happen if copying errors occur during DNA replication?

<p>Base changes or mutations in the DNA would result (D)</p> Signup and view all the answers

Which direction can DNA polymerase enzymes synthesize DNA?

<p>5′ to 3′ only (B)</p> Signup and view all the answers

Which type of DNA ends are not suitable for exonucleases to work on?

<p>Closed circular DNA (D)</p> Signup and view all the answers

What substrate requirement affects the activity of exonuclease I from E.coli?

<p>Single-stranded DNA (A)</p> Signup and view all the answers

Which type of DNA ends can Exonuclease III efficiently remove mononucleotides from?

<p>Recessed ends (C)</p> Signup and view all the answers

What additional activity does Exo III have besides removing mononucleotides from DNA ends?

<p>Cutting DNA at apurinic sites (A)</p> Signup and view all the answers

When using Exonuclease VII from E.coli, which type of DNA ends can be digested by this enzyme?

<p>3′-hydroxyl ends (A)</p> Signup and view all the answers

For what purpose was Exo III used in the research setting according to the text?

<p>Creating nested deletions (D)</p> Signup and view all the answers

What is a unique feature of Exo VII among enzymes?

<p>5′ exonuclease activity (B)</p> Signup and view all the answers

How can Exo VII be utilized in DNA manipulation?

<p>To remove long single strands from DNA (A)</p> Signup and view all the answers

What was a limitation of the initial method used for joining separate DNA helices?

<p>It required the chance positioning of two adjacent ends (B)</p> Signup and view all the answers

How did H. Gobind Khorana demonstrate efficient joining of DNA fragments?

<p>By employing short synthetic segments with single-strand complementary overhangs (A)</p> Signup and view all the answers

What was observed to increase the efficiency of joining the ends of DNA molecules from certain bacterial viruses?

<p>Treating DNA ends with terminal transferase (C)</p> Signup and view all the answers

Why was hydrogen bonding between single strands considered important for efficient ligation?

<p>To hold ends together before ligation (B)</p> Signup and view all the answers

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