Podcast
Questions and Answers
What is agarose gel electrophoresis used for?
What is agarose gel electrophoresis used for?
- Purification of DNA samples
- Quantification of RNA molecules
- Separation of DNA, RNA, and sometimes large proteins (correct)
- Amplification of DNA sequences
What is the concentration range of agarose used in agarose gel electrophoresis?
What is the concentration range of agarose used in agarose gel electrophoresis?
- 2 to 5 percent
- 5 to 8 percent
- 0.8 to 2 percent (correct)
- 0.2 to 0.5 percent
What is the purpose of adding ethidium bromide (EtBr) to the agarose gel?
What is the purpose of adding ethidium bromide (EtBr) to the agarose gel?
- To prevent DNA degradation
- To increase the resolution of protein bands
- To provide a pH buffer
- To visualize DNA under UV light (correct)
What is the function of bromophenol blue in agarose gel electrophoresis?
What is the function of bromophenol blue in agarose gel electrophoresis?
What is the purpose of the buffer used in the buffer tank during agarose gel electrophoresis?
What is the purpose of the buffer used in the buffer tank during agarose gel electrophoresis?
What are the alterations primary transcripts undergo during post-transcriptional modifications?
What are the alterations primary transcripts undergo during post-transcriptional modifications?
What is the primary transcript of mRNA in eukaryotes called?
What is the primary transcript of mRNA in eukaryotes called?
What is responsible for the processing of tRNAs and rRNAs in both prokaryotes and eukaryotes?
What is responsible for the processing of tRNAs and rRNAs in both prokaryotes and eukaryotes?
What modification occurs at the 5’ end of mRNA during post-transcriptional processing?
What modification occurs at the 5’ end of mRNA during post-transcriptional processing?
In contrast to prokaryotic mRNA, what undergoes extensive post-transcriptional changes in eukaryotes?
In contrast to prokaryotic mRNA, what undergoes extensive post-transcriptional changes in eukaryotes?
