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Agarose Gel Electrophoresis Basics Quiz

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Questions and Answers

What is agarose gel electrophoresis used for?

Purification of DNA samples

Quantification of RNA molecules

Separation of DNA, RNA, and sometimes large proteins (correct)

Amplification of DNA sequences

What is the concentration range of agarose used in agarose gel electrophoresis?

2 to 5 percent

5 to 8 percent

0.8 to 2 percent (correct)

0.2 to 0.5 percent

What is the purpose of adding ethidium bromide (EtBr) to the agarose gel?

To prevent DNA degradation

To increase the resolution of protein bands

To provide a pH buffer

To visualize DNA under UV light (correct)

What is the function of bromophenol blue in agarose gel electrophoresis?

To monitor the progress of electrophoresis Signup and view all the answers

What is the purpose of the buffer used in the buffer tank during agarose gel electrophoresis?

To provide a medium for ion movement Signup and view all the answers

What are the alterations primary transcripts undergo during post-transcriptional modifications?

Terminal base additions, base modifications, splicing Signup and view all the answers

What is the primary transcript of mRNA in eukaryotes called?

hnRNA Signup and view all the answers

What is responsible for the processing of tRNAs and rRNAs in both prokaryotes and eukaryotes?

Ribonucleases Signup and view all the answers

What modification occurs at the 5’ end of mRNA during post-transcriptional processing?

Capping with 7-methylguanosine Signup and view all the answers

In contrast to prokaryotic mRNA, what undergoes extensive post-transcriptional changes in eukaryotes?