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Questions and Answers
What is agarose gel electrophoresis primarily used for?
What is agarose gel electrophoresis primarily used for?
What is agarose derived from?
What is agarose derived from?
How are molecules separated in agarose gel electrophoresis?
How are molecules separated in agarose gel electrophoresis?
In agarose gel electrophoresis, towards which direction do negatively charged particles move?
In agarose gel electrophoresis, towards which direction do negatively charged particles move?
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Why do biomolecules move at different rates in electrophoresis?
Why do biomolecules move at different rates in electrophoresis?
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What characteristic of biomolecules determines their direction of migration in an electric field?
What characteristic of biomolecules determines their direction of migration in an electric field?
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Study Notes
Agarose Gel Electrophoresis
- A method of gel electrophoresis used in molecular biology, genetics, and clinical chemistry to separate macromolecules such as DNA, RNA, or proteins.
- Uses a matrix of agarose, a natural linear polysaccharide extracted from seaweed.
Principle of Electrophoresis
- Separation based on electrophoretic charge, which depends on the extra functional group on the surface of biomolecules.
- Biomolecules exist as electrically charged particles with either positive or negative charges.
- In an electric field, charged molecules travel to the opposite direction of the positive or negative pole.
- Molecules migrate at different rates depending on their mass, not their charge, when exposed to an electric field.
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Description
Learn about agarose gel electrophoresis, a method used to separate macromolecules like DNA, RNA, and proteins in molecular biology, genetics, and clinical chemistry. Understand the principle and process of agarose gel electrophoresis.
