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Questions and Answers
In agarose gel electrophoresis, what does a higher concentration of agarose result in?
In agarose gel electrophoresis, what does a higher concentration of agarose result in?
Which of the following factors does NOT affect the migration rate of DNA in gel electrophoresis?
Which of the following factors does NOT affect the migration rate of DNA in gel electrophoresis?
What is the purpose of using a DNA ladder in gel electrophoresis?
What is the purpose of using a DNA ladder in gel electrophoresis?
Which of the following statements is true about polyacrylamide gel electrophoresis (PAGE)?
Which of the following statements is true about polyacrylamide gel electrophoresis (PAGE)?
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What is the typical charge of proteins that allows them to be separated by gel electrophoresis?
What is the typical charge of proteins that allows them to be separated by gel electrophoresis?
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Which of the following methods is NOT used to increase the resolution of smaller DNA fragments in electrophoresis?
Which of the following methods is NOT used to increase the resolution of smaller DNA fragments in electrophoresis?
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Study Notes
Agarose Gel Electrophoresis
- A higher concentration of agarose results in slower migration of large DNA fragments and greater resolution of small DNA fragments.
- Factors that affect the migration rate of DNA in gel electrophoresis include voltage applied, size of DNA fragments, and temperature of the gel.
- The concentration of DNA does not affect the migration rate.
DNA Ladder
- The purpose of using a DNA ladder in gel electrophoresis is to measure the size of the DNA fragments.
Polyacrylamide Gel Electrophoresis (PAGE)
- PAGE provides higher resolution than agarose gel.
- PAGE is suitable for protein separation.
Gel Electrophoresis of Proteins
- Proteins can be separated by gel electrophoresis due to their typical negative charge.
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Description
This quiz is designed to test your knowledge of molecular biology concepts, including agarose gel electrophoresis and DNA migration.
