Questions and Answers
In agarose gel electrophoresis, what does a higher concentration of agarose result in?
Which of the following factors does NOT affect the migration rate of DNA in gel electrophoresis?
What is the purpose of using a DNA ladder in gel electrophoresis?
Which of the following statements is true about polyacrylamide gel electrophoresis (PAGE)?
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What is the typical charge of proteins that allows them to be separated by gel electrophoresis?
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Which of the following methods is NOT used to increase the resolution of smaller DNA fragments in electrophoresis?
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Study Notes
Agarose Gel Electrophoresis
- A higher concentration of agarose results in slower migration of large DNA fragments and greater resolution of small DNA fragments.
- Factors that affect the migration rate of DNA in gel electrophoresis include voltage applied, size of DNA fragments, and temperature of the gel.
- The concentration of DNA does not affect the migration rate.
DNA Ladder
- The purpose of using a DNA ladder in gel electrophoresis is to measure the size of the DNA fragments.
Polyacrylamide Gel Electrophoresis (PAGE)
- PAGE provides higher resolution than agarose gel.
- PAGE is suitable for protein separation.
Gel Electrophoresis of Proteins
- Proteins can be separated by gel electrophoresis due to their typical negative charge.
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Description
This quiz is designed to test your knowledge of molecular biology concepts, including agarose gel electrophoresis and DNA migration.
