Agar Preparation for Gram Staining

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Questions and Answers

What is the purpose of heating the inoculating loop until red and then cooling it down?

  • To mix the agar with distilled water
  • To observe the bacteria under the microscope
  • To sterilize the loop (correct)
  • To prepare the sample for streak plating

What is the ratio of agar powder to distilled water for making agar?

  • 8.6g/300mL (correct)
  • 5g/100mL
  • 1g/10mL
  • 28g/L

What is the purpose of pouring the agar mixture into a petri dish while placing the lid of the flask and petri dish above the alcohol lamp?

  • To prevent contamination (correct)
  • To mix the agar with distilled water
  • To observe the sample under the microscope
  • To incubate the sample

What is the purpose of smearing the sample onto the sterile petri dish?

<p>To streak the plate (A)</p> Signup and view all the answers

What is the purpose of boiling the hay or grass until it smells like tea?

<p>To make hay infusion (D)</p> Signup and view all the answers

What is the purpose of adding iodine in the gram staining procedure?

<p>To mordant the sample (B)</p> Signup and view all the answers

To make agar, mix ______ grams of agar powder with 1 liter of distilled water.

<p>28</p> Signup and view all the answers

Before pouring the agar mixture into a petri dish, cool it down to ______ temperature.

<p>room</p> Signup and view all the answers

In the streak plate method, use a ______ stick to pick up any sample from the environment.

<p>swab</p> Signup and view all the answers

Heat the ______ loop until it turns red and then cool it down before use.

<p>inoculating</p> Signup and view all the answers

In the gram staining procedure, add ______ Violet to the sample for 30 seconds.

<p>Crystal</p> Signup and view all the answers

After pouring the agar mixture into a petri dish, put it in the ______ to sterilize it.

<p>autoclave</p> Signup and view all the answers

In the hay infusion method, use ______ water to make the infusion.

<p>canal</p> Signup and view all the answers

To make hay infusion, boil the hay or grass until it smells like ______.

<p>tea</p> Signup and view all the answers

Use a ______ to scrape the bacteria from the petri dish in the gram staining procedure.

<p>inoculating loop</p> Signup and view all the answers

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