Lec4_ISE_and_gas_analyzer.pdf

Ion Selective Electrode Ion selective electrode is a widely used analytical tool for sensing ions in various clinical or research samples These are membrane electrode, that acts as a sensor, that converts the activity of a specific ion into an electrical potential. What is an ion selective electro...

Ion Selective Electrode Ion selective electrode is a widely used analytical tool for sensing ions in various clinical or research samples These are membrane electrode, that acts as a sensor, that converts the activity of a specific ion into an electrical potential. What is an ion selective electrode? Ion selective electrodes are the electrode that generates potential in the response to the presence of a solution of a specific ion. Since this electrode detects specific ions only, it can also be considered a sensor. Ion selective electrode principle An ideal ISE consists of a thin membrane across which only the specific ion can be transported. The transport of ions from a high concentration to a low concentration one through selective binding with some sites within the membrane creates a potential difference, this is the fundamental principle of ISE. Glass membrane electrode ——————————————Glass membrane electrodes are used for sensing univalent cations such as H+, Na+etc. Applications of Ion Selective electrode To determine ions like ca++, K+, Na+ , Cl– etc in body fluids like plasma, serum, and sweat. Advantages of ion selective ———————————-electrode —————- Simple, low-cost, and easy to handle. It can be used for the analysis of a continuous flow system. Arterial Blood Gas Analyzer Arterial Blood Gas Analysis measures blood gas tension, arterial partial pressure of oxygen (PaO2) and carbon dioxide (PaCO2), pH and oxygen saturation (SaO2). The machine used for analysis aspirates this blood from the syringe and measures the pH and the partial pressures of oxygen and carbon dioxide. The bicarbonate concentration is also calculated. These results are usually available for interpretation within five minutes cobas b 221 system - Roche Diagnostics The cobas b 221 system is a quick and reliable blood gas and electrolyte analyzer which measures 18 key critical care parameters An acceptable normal range of ABG values of ABG components are the following, noting that the range of normal values may vary among laboratories and in different age groups from neonates to geriatrics: pH (7.35-7.45) PaO2 (75-100 mmHg) PaCO2 (35-45 mmHg