Ion Selective Electrodes in Analytical Chemistry

Questions and Answers

To measure the pH of a solution

To convert the activity of a specific ion into an electrical potential (correct)

To calculate the bicarbonate concentration in blood

To detect the presence of all ions in a solution

Poruous membrane

Glass membrane

Semipermeable membrane (correct)

Ion-impermeable membrane

Spectrophotometric detection of ions

Electrochemical reaction between the ion and the electrode

Transport of ions from a high concentration to a low concentration through selective binding (correct)

Chromatographic separation of ions

Simple, low-cost, and easy to handle Signup and view all the answers

Arterial Blood Gas Analyzer Signup and view all the answers

Blood gas tension, arterial partial pressure of oxygen, carbon dioxide, pH, and oxygen saturation Signup and view all the answers

Bicarbonate concentration Signup and view all the answers

Univalent cations Signup and view all the answers

Study Notes

An ion selective electrode (ISE) is a membrane electrode that senses ions in various clinical or research samples, converting the activity of a specific ion into an electrical potential.
ISEs generate potential in response to the presence of a solution of a specific ion, detecting specific ions only, making them sensors.
The principle of ISE involves a thin membrane that allows only the specific ion to be transported, creating a potential difference through selective binding with sites within the membrane.

Glass membrane electrodes are used for sensing univalent cations such as H+, Na+, etc.

ISEs are used to determine ions like Ca++, K+, Na+, Cl–, etc. in body fluids like plasma, serum, and sweat.

Arterial Blood Gas Analysis measures blood gas tension, arterial partial pressure of oxygen (PaO2) and carbon dioxide (PaCO2), pH, and oxygen saturation (SaO2)
The machine used for analysis aspirates blood from a syringe and measures pH and the partial pressures of oxygen and carbon dioxide
Bicarbonate concentration is also calculated