Ion Selective Electrodes in Analytical Chemistry
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Questions and Answers

What is the primary function of an ion selective electrode?

  • To measure the pH of a solution
  • To convert the activity of a specific ion into an electrical potential (correct)
  • To calculate the bicarbonate concentration in blood
  • To detect the presence of all ions in a solution

    • What type of membrane is used in glass membrane electrodes?

  • Poruous membrane
  • Glass membrane
  • Semipermeable membrane (correct)
  • Ion-impermeable membrane

    • What is the principle behind the operation of an ion selective electrode?

  • Spectrophotometric detection of ions
  • Electrochemical reaction between the ion and the electrode
  • Transport of ions from a high concentration to a low concentration through selective binding (correct)
  • Chromatographic separation of ions

    • What is an advantage of using ion selective electrodes?

    <p>Simple, low-cost, and easy to handle</p> Signup and view all the answers

    What is the name of the machine used for Arterial Blood Gas Analysis?

    <p>Arterial Blood Gas Analyzer</p> Signup and view all the answers

    What is measured by an Arterial Blood Gas Analyzer?

    <p>Blood gas tension, arterial partial pressure of oxygen, carbon dioxide, pH, and oxygen saturation</p> Signup and view all the answers

    What is calculated by an Arterial Blood Gas Analyzer?

    <p>Bicarbonate concentration</p> Signup and view all the answers

    What type of ions can be sensed using glass membrane electrodes?

    <p>Univalent cations</p> Signup and view all the answers

    Study Notes

    Ion Selective Electrode

    • An ion selective electrode (ISE) is a membrane electrode that senses ions in various clinical or research samples, converting the activity of a specific ion into an electrical potential.
    • ISEs generate potential in response to the presence of a solution of a specific ion, detecting specific ions only, making them sensors.
    • The principle of ISE involves a thin membrane that allows only the specific ion to be transported, creating a potential difference through selective binding with sites within the membrane.

    Glass Membrane Electrode

    • Glass membrane electrodes are used for sensing univalent cations such as H+, Na+, etc.

    Applications of Ion Selective Electrode

    • ISEs are used to determine ions like Ca++, K+, Na+, Cl–, etc. in body fluids like plasma, serum, and sweat.

    Advantages of Ion Selective Electrode

    • Simple, low-cost, and easy to handle
    • Can be used for the analysis of a continuous flow system

    Arterial Blood Gas Analysis

    • Arterial Blood Gas Analysis measures blood gas tension, arterial partial pressure of oxygen (PaO2) and carbon dioxide (PaCO2), pH, and oxygen saturation (SaO2)
    • The machine used for analysis aspirates blood from a syringe and measures pH and the partial pressures of oxygen and carbon dioxide
    • Bicarbonate concentration is also calculated

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    Ion Selective Electrode & Blood Gas Analyzer PDF

    Description

    Learn about ion selective electrodes, a crucial tool in analytical chemistry used to detect specific ions in clinical and research samples.

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