Bacterial Sample Collection Lab. No. 1 PDF
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This document details various techniques for collecting bacterial samples and different methods of sterilization for lab materials and tools. It covers sample types, physical sterilization methods like heat and radiation, and chemical sterilization methods. The lab notes also describe the importance of understanding pathogen types to determine suitable sterilization techniques for different materials and samples.
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F Lab. No.1. Bacterial sample collection' Sample types...
F Lab. No.1. Bacterial sample collection' Sample types il ". F;i !-.r::: ' ': -we should determined the infected area. 1-Throat swab 2-Ear swab \-, 3-Nasalswab 4-Nasal secretion S-SPutum samPle r 6-BiobsY samPle 7-Peritoneal fluid samPle 8-Vaginal swab (high or cervical) 9-Semenal,prostatic, urethral discharge l0-StoolsamPle r 11-Urin samPle r 12-Blood samPle r 13-Wound swab 14-Skin swab L5-Cerebro spinal fluid sample (CSF) NoU we should know type of sample (infected area),suspect type of pathogens determine type of media that will be use for cultivating.we must make direct examination of sample immediatelY if we need. Method; we use the collection media as swab media to collect some swabs as the student like (ear, nasal, skin, mouth) swab. + f> it-3 ? 151/ Sterilization organisms exist the complete removal or eradicate of all micro- in Sterilization method: is fungi or above materials and tools including vegetative bactenia, bacterial spores, viruses , and protozoa. 6 There are many techniques used to sterilize materials and laboratory tools , these are: A-physical sterilization methods: i 1-Heat: a-dry heat: l--incineration: by using of flame ,ex; flaming of loop, needle, slides. 2- hot air: by using of oven at 1-60-180 c for 90-120 min, ex; powder material (heat resistant), soccer scissors forceps and other related tools, glass wares like Petri-dishes and pipettes....etc, b-moist heat: 1-steem under pressure: by using of Auto clave in 121c at L5 par for t5-2O min, used for media and all heat resistant materials and fluid. 2-steem: by using Arnold sterilization system at 100c for L5min in three flowing days this method called fractional sterilization use for sugar solution and all materials destroy above 100c. c-boiling: heating of item to 1"00c,by this way killed only vegetative cells. d-pasteurization: usage of liquid material at 63c for 30 min or 72ctor 20 min ex: milk and other protein materials. 2- Radiation: Using of Gamma, X , ultra violet (U.V) and Beta rays ,use for sterilizing plastic tools, surgical rooms....etc. B-chemical sterilization methods : by using ethyl , methyl and isopropyl alcohol ex:75To of ethanol used for cleaning & sterilizing surface and hands, skin antiseptic before injection or venepuncture. Also use of 2-5% phenol for cleaning floor and surface of tables ,bench.... etc. Other chemicals ;aldehydes ,halogens and others C-mechanicals sterilization methods: by using specific filters (bacteriological filter) ex: Seitz , Berkfeld, membrane filters and others, used for sterilizing liquids have heat sensitive materials like biological fluids (serum) and solution of enzyme ,vitamin and antibiotic. Not ;these filter have specific pores (0.45) prevent passing of microbial cells charge of filter and microbial cells and liquid nature is ver\7 important. Methods: culture of swap sample from tables directly and after cleaning with ethanol (75%)using of nutrient agar. #
