Methods Exam PDF - Biomedicine Exam Paper

Methods-exam.pdf biomedicine Methods and Techniques in Biomedicine i 2º Grado en Biomedicina Facultad de Ciencias de la Salud y del Deporte Universidad Alfonso X El Sabio Reservados todos los derechos. No se permi...

Methods-exam.pdf biomedicine Methods and Techniques in Biomedicine i 2º Grado en Biomedicina Facultad de Ciencias de la Salud y del Deporte Universidad Alfonso X El Sabio Reservados todos los derechos. No se permite la explotación económica ni la transformación de esta obra. Queda permitida la impresión en su totalidad.  1- In the developing of SDS-PAGE a researcher has forgotten to add glycerol to the loading buffer, it is likely that: 2- A researcher wants to analyze a protein of 200kDa molecular mass. An appropriate separating gel would have a final acrylamide percentage of: a) 7% b) 1% c) 20% d) 5% 3- The function of correcting the densitometry by using the value of the loading control is to: a) normalize the signal to obtain a ratio that allows to increase the specific signal b) normalize the signal, to obtain a ratio between the densitometry value and the biological changes c) normalize the signal, to obtain a ratio between the change caused by the experiment and a known and relatively stable signal value d) normalize the signal to obtain a ratio to decrease the background 4- An important variable when selecting restriction sites to initially open a plasmid would be a) find multiple restriction sites to ensure that the restriction enzyme will find them (at least one of them) b) find a restriction site that is unique to prevent the plasmid from recircularization c) find two restriction sites that are unique to prevent the vector from recircularization d) find two unique restriction sites to allow easier insertion of the gene of interest 5- A researcher is looking to generate a stable cell line, what type of vector would you advise using? a) third-generation plasmid vector b) viral vector c) third generation viral vector with HA tag d) plasmid vector with neomycin resistance 6- One way to ensure the correct expression of a eukaryotic protein (without post-translational modifications) in a prokaryotic system could be to generate a cloning experiment from… a) eukaryotic proteins cannot be obtained in prokaryotic systems b) the eukaryotic gene and using a bacterial backbone c) eukaryotic mRNA and using a bacterial backbone d) a eukaryotic cDNA and using a bacterial backbone 7- A researcher wants to analyze a protein of 200kDa molecular mass. An appropriate concentrating gel would have a final acrylamide percentage of: a) 7% b) 1% c) 20% a64b0469ff35958ef4ab887a898bd50bdfbbe91a-7247987 Reservados todos los derechos. No se permite la explotación económica ni la transformación de esta obra. Queda permitida la impresión en su totalidad.  d) 5% 8- A researcher seeks to clone the insulin gene to produce recombinant insulin and to purify it in order to use in his/her experiments of glucose regulation. The sequence of interest to insert into the appropriate vector should be a) the sequence of the insulin gene b) the sequence of the introns of the insulin gene c) the sequence of the mRNA (as cDNA) d) the protein sequence after post translational processing 9- A researcher is analyzing by Western Blot (WB) the phosphorylation of AKT. As for blocking he/she should a) check if the use of BSA affects the signal sought in the WB b) check if the use of skim milk affects the signal sought in the WB c) use 5% skim milk because its blocking action is adequate d) use BSA as its blocking action is usually better than skim milk 10- A researcher is about to generate a figure from Western Blot (WB) results in a siRNA experiment. In one lane, there is a control siRNA (that do not knockdown any gene) and in the other lane, there is a siRNA positive treatment. However, researcher observe differences in the signal of the loading controls. Knowing that WB is semiquantitative, the approximation you could propose to compose the figure would be a) to densitometrate the bands and obtain an average of the signals for each lane b) to densitometrate the bands and correct its values with the control siRNAs signal c) to discard the experiment and start again d) to densiometrate the bands and correct its values with the loading controls´ signal 11- In a WB experiment, blocking of the membrane was not optimal, thus is likely that a) the primary antibody will not interact properly b) there would be no major problems c) the secondary antibody will be blocked from interacting d) there will be higher background 12- In terms of ethics in scientific image manipulation, the next one is generally considered a good practice a) performing partial manipulations to our images to highlight a result b) enhance the background of the images to remove elements and/or better highlight our results c) making small adjustments to make our data better fit our hypotheses d) cutting out a portion of the total image, if the procedure performed is indicated 13- The correct position of the layers of the WB “sandwich” starting from close to the negative electrode and moving towards the positive electrode is a) sponge, membrane, filter paper, gel, sponge, filter paper b) sponge, filter paper, gel, membrane, filter paper, sponge c) sponge, filter paper, membrane, gel, filter paper, sponge a64b0469ff35958ef4ab887a898bd50bdfbbe91a-7247987 Reservados todos los derechos. No se permite la explotación económica ni la transformación de esta obra. Queda permitida la impresión en su totalidad.  d) filter paper, sponge, membrane, filter paper, sponge 14- One way to purify recombinant proteins obtained in prokaryotic systems could be to perform a cloning… a) with an unspecific tag and using magnetic beads b) with a natural fluorophore and observe ir under the microscope c) with a specific tag and then perform a DNA purification d) with a specific tag and then performing an immunoprecipitation a64b0469ff35958ef4ab887a898bd50bdfbbe91a-7247987 Reservados todos los derechos. No se permite la explotación económica ni la transformación de esta obra. Queda permitida la impresión en su totalidad.