Unit 2 Support Services PDF

Unit 2 Support Services Baltazar Vazquez Microbiology Laboratory Technician Agenda Lab Supplies obtaining, receiving, storing and inventory Glassware Preparation Specimen Receiving, processing, and distribution Media Prep Sterilization methods (Physical and Chemical) Proper Decontamina...

Unit 2 Support Services Baltazar Vazquez Microbiology Laboratory Technician Agenda Lab Supplies obtaining, receiving, storing and inventory Glassware Preparation Specimen Receiving, processing, and distribution Media Prep Sterilization methods (Physical and Chemical) Proper Decontamination and Disposal Quality of Laboratory Water Lab Supplies Obtaining: Sourcing from reputable suppliers, bulk purchasing benefits. Receiving: Inspect upon receipt, documentation. Storing: Optimal conditions (temperature and humidity), organization. Inventory: regular audits, tracking systems, reorder points. Some things to think about when ordering Lab supplies is: Purchasing guidelines may vary by department How fast items get used How long are the expiration dates Shorter Exp. Date: order a little bit more than needed and more frequent Longer Exp. Date: order enough and less frequent Always consider how long order process takes when reordering. If bulk order discounts are worth over stocking (usually worth it with long Exp. or no Exp. How long it takes for items to be delivered (delivery time is key when reordering items with EXP dates) Awareness of space available to store items (refrigerator, freezer, and room temp space in lab) Storage requirements are specific to items (always check packaging or instruction for Temp and Humidity requirements) Reagents, Media, Flammables, Anaerobic media, Acids, and Gas Cylinders Safety Factor Lab Supplies Lab Supplies Obtaining: Sourcing from reputable suppliers, bulk purchasing benefits. Receiving: Inspect upon receipt, documentation. Storing: Optimal conditions (temperature and humidity), organization. Inventory: regular audits, tracking systems, reorder points. Glassware Prep Cleaning Protocols: Manual vs. Automated. Drying: Air vs Oven Sterilization Inspection for defects or contamination Glassware Prep Manual: Rinse with warm water to remove lose particles. Use mild detergent and scrub using designated brushes to avoid scratching the glass. Rinse thoroughly to remove all soap residue. Automated: Pre-rinse or scrape off heavy residues. Load glassware correctly to prevent chipping or contact with other glassware. Select the appropriate cycle. Unload carefully to avoid accidents. Air Drying: Leave glassware on racks to let dry. Oven Drying: Glassware is placed in a drying oven at a temperature suitable for all types of glass.  Glassware Prep Pros and cons Manual washing Automated washing Air Drying Oven Drying PROS Control and customize Consistent Simple Fast process wash Efficient No equipment needed Completely dry Great for specialized and Large volume load No energy needed Great for narrow delicate glassware Enhanced cycles tubes and crevices Immediate wash Less time and labor CONS Labor intensive Cost of machines Slow process Cost of machine Inconsistent Not suitable for all Not completely dried Not suitable for all Time consuming glassware Risk of dust or airborne items Risk of breaking or being Maintenance contaminants setting in Energy usage injured because of Maintenance glassware Glassware Prep Sterilization Dry: Foil Common setting include temperatures from 160-200°C using heat. Good for cylinders, open flask, and beakers. Always attach a dry heat indicator. Autoclave: Plastic or Metal Caps Cap loosely and place autoclave tape and/or autoclave indicators, to indicate sterilization. Autoclave at 15 psi, 121°C for 15 to 30 min.  Physical Advantages Limitation Chemical Advantages Limitation Autoclavin High Temperatures can be Are not suitable for Hydrogen Non-toxic byproducts, Material g reached. heat-sensitive objects Peroxide Gas Wide material compatibility Steam is an effective way Repeated exposure to compatibility, limitations, Chamber to transfer heat. heat and humidity can Rapid cycle time, Low size constraints, Pre- Penetrates Materials dull sharp objects. temperature sterilization, processed Effective for materials that and Easy to monitor. requirement, contain water. Surface penetration Dry Heat Non-corrosive, wide Time consuming, issues, and material compatibility, Easy Damages or warps Exposure to to operate and maintain, sensitive materials, operator. Cost effective, Deep Requires more energy, penetration, Can’t sterilize environmentally friendly, flammable materials. and Flexible installation. Filtration Heat sensitive application, Clogging potential, Effective against large Filter integrity, Not microbes, Simple process, effective on smaller Can be used in large microbes or viruses, volumes. Cost of filters, and Potential absorption. Sterilization Methods Specimen Receiving, processing, and distribution Specimen Collection: Method of sample collection, labeling, Correct submitter form (information with storage requirement, preservation and shipment method). Receiving: Chain of custody, Initial inspection (Check for crack or leaks). Processing: Opening packages in BSC, Labeling, sorting, initial preparation, secured in racks if needed, put into proper carriers, submitter form and lab forms organized for each sample Distribution: Distribute on a cart for multiple specimen to their respective labs. Media Prep Types of media used in lab. Preparation steps: Wear correct PPE, read recipe card multiple times, clean glassware, weighing accurately, microbiology grade water, mixing until homogeneous, Adjust pH if needed, sterilizing time and pressure as directed in recipe card and do not overheat. Labeling: Name of media, date made, expiration date, and storage information. Quality control before use: Never use expired media when conducting diagnostic work. Proper Decontamination Follow SOP from respective lab. Decontamination procedures for different lab materials. Safe disposal practice aligned with regulations. Importance of waste segregation. Quality of Laboratory Water Importance of Water Quality: Impact on experiments, equipment, reliability and accuracy of results. Testing Methods: 1. Conductivity: Measures ionic content, indicative of purity. 2. pH Testing: Ensures water is neutral or at the required pH. 3. Bacterial Count: Regular testing for microbial contamination. 4. Total Organic Carbon (TOC): Detects organic impurities. Frequency: Daily, monthly, or quarterly. Equipment: Use specific water purity and pH meters, filtration checks. Conclusion Lab support services are crucial to a lab Everything has its place from glassware to the quality of water used