Podcast
Questions and Answers
What is a crucial step when receiving laboratory supplies?
What is a crucial step when receiving laboratory supplies?
- Assuming all items are correct without verification.
- Inspecting items upon receipt and documenting any discrepancies. (correct)
- Disposing of packing materials before verifying the contents.
- Immediately placing items into storage without inspection.
When ordering lab supplies with shorter expiration dates, what approach is generally preferred?
When ordering lab supplies with shorter expiration dates, what approach is generally preferred?
- Ordering slightly more than needed and more frequently. (correct)
- Ordering less than needed to avoid waste if the expiration date arrives soon.
- Ignoring the expiration date as they are usually very conservative.
- Ordering a large quantity to minimize the frequency of reordering.
What consideration is crucial when deciding to purchase lab supplies in bulk?
What consideration is crucial when deciding to purchase lab supplies in bulk?
- If there is space available in the office to store the bulk orders.
- The current trends in laboratory supply purchasing.
- The color of the packaging.
- Whether the bulk order provides discounts that outweigh the risk of overstocking. (correct)
Which factor should be considered when storing laboratory supplies, according to the text?
Which factor should be considered when storing laboratory supplies, according to the text?
What is the initial step in manual cleaning of glassware?
What is the initial step in manual cleaning of glassware?
In automated glassware cleaning, what is one of the key considerations prior loading?
In automated glassware cleaning, what is one of the key considerations prior loading?
When air drying glassware, what is the recommended practice?
When air drying glassware, what is the recommended practice?
What role does documentation play when receiving lab supplies?
What role does documentation play when receiving lab supplies?
Which of the following is a disadvantage of using dry heat sterilization?
Which of the following is a disadvantage of using dry heat sterilization?
What is a key advantage of using filtration for sterilization?
What is a key advantage of using filtration for sterilization?
What step should be immediately performed when receiving a specimen in the lab?
What step should be immediately performed when receiving a specimen in the lab?
What is the most important factor when preparing media in a microbiology lab?
What is the most important factor when preparing media in a microbiology lab?
What kind of information should be included on the label of a prepared media?
What kind of information should be included on the label of a prepared media?
Why is water quality crucial in a laboratory setting?
Why is water quality crucial in a laboratory setting?
What does conductivity measure in the context of laboratory water?
What does conductivity measure in the context of laboratory water?
What is an important aspect to consider for decontamination?
What is an important aspect to consider for decontamination?
Which of the following is a primary advantage of using an oven for drying glassware?
Which of the following is a primary advantage of using an oven for drying glassware?
A laboratory technician needs to sterilize a batch of glass beakers and cylinders. Which sterilization method is best suited for this task?
A laboratory technician needs to sterilize a batch of glass beakers and cylinders. Which sterilization method is best suited for this task?
What is a limitation of relying solely on air drying for glassware?
What is a limitation of relying solely on air drying for glassware?
When using an autoclave for sterilization, which of the following is a critical requirement?
When using an autoclave for sterilization, which of the following is a critical requirement?
What is a significant drawback of manual washing of glassware in a laboratory setting?
What is a significant drawback of manual washing of glassware in a laboratory setting?
Which of the following is a limitation of using hydrogen peroxide gas for sterilization?
Which of the following is a limitation of using hydrogen peroxide gas for sterilization?
What is one advantage of automated washing systems compared to manual washing?
What is one advantage of automated washing systems compared to manual washing?
Why is it necessary to use indicators when sterilizing glassware?
Why is it necessary to use indicators when sterilizing glassware?
Flashcards
Lab Supply Obtaining
Lab Supply Obtaining
The process of obtaining lab supplies from reliable sources and leveraging the cost savings of purchasing in larger quantities.
Lab Supply Receiving
Lab Supply Receiving
Verifying the quality and quantity of received lab supplies, documenting their arrival, and maintaining accurate records.
Lab Supply Storing
Lab Supply Storing
Storing lab supplies in a way that maintains their quality and ensures their longevity. This often involves specific temperatures and humidity controls.
Lab Supply Inventory
Lab Supply Inventory
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Purchasing Guidelines
Purchasing Guidelines
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Glassware Cleaning
Glassware Cleaning
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Glassware Sterilization
Glassware Sterilization
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Glassware Inspection
Glassware Inspection
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Oven Drying
Oven Drying
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Autoclaving
Autoclaving
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Hydrogen Peroxide Gas Sterilization
Hydrogen Peroxide Gas Sterilization
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Dry Heat Sterilization
Dry Heat Sterilization
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Manual Glassware Washing
Manual Glassware Washing
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Automated Glassware Washing
Automated Glassware Washing
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Air Drying
Air Drying
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Autoclave Sterilization for Caps
Autoclave Sterilization for Caps
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Specimen Collection
Specimen Collection
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Specimen Receiving
Specimen Receiving
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Specimen Processing
Specimen Processing
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Specimen Distribution
Specimen Distribution
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Media Prep
Media Prep
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Decontamination
Decontamination
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Water Quality
Water Quality
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Conductivity Testing
Conductivity Testing
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Study Notes
Unit 2 Support Services
- This unit covers critical lab support functions.
Agenda
- Lab Supplies: Obtaining, receiving, storing, and inventory management.
- Glassware Preparation: Cleaning protocols (manual vs. automated), drying (air vs. oven), and sterilization procedures.
- Specimen Receiving, processing, and distribution: Chain of custody, initial inspection, processing, preparation, and distribution.
- Media Preparation: Types of media used, preparation steps, labeling, and quality control.
- Sterilization methods: Dry heat (foil) and autoclave (plastic/metal caps).
- Proper Decontamination and Disposal: Following SOPs, material-specific decontamination procedures, and safe disposal.
- Quality of Laboratory Water: Importance, testing methods (conductivity, pH, bacterial count, TOC), and frequency of testing, along with necessary equipment.
Lab Supplies
- Obtain supplies from reputable suppliers for bulk purchasing.
- Inspect supplies upon receipt, document properly.
- Store supplies in optimal conditions (temperature and humidity) and organize them.
- Use regular audits, inventory tracking systems, and reorder points.
- Ordering Considerations: Purchasing guidelines, item usage rate, expiration dates, order lead time, and appropriate bulk ordering strategies.
Glassware Preparation
- Manual cleaning: Rinsing with warm water, mild detergent, and specific brushes, thorough rinsing.
- Automated cleaning: Pre-rinsing/scraping, loading glassware, proper cycle selection, and careful unloading.
- Drying: Air drying or oven drying, selecting appropriate temperature settings.
- Inspection for defects or contamination.
Glassware Prep: Pros and Cons (Manual vs. Automated)
- Manual: Control and customization, specialized handling of delicate glassware, immediate washing.
- Cons: Labor intensive, time-consuming, inconsistent, risk of breakage.
- Automated: Consistent, efficient, handling of large volumes, reduced time.
- Cons: Cost of machines, less time/labor control, possibly not suitable for all types of glassware, possible maintenance.
- Air Drying: Simple, does not require equipment,
- Cons: Slow process, may not completely dry.
- Oven Drying: Fast, complete drying,
- Cons: Cost of machines, not suitable for all items, may require energy.
Glassware Prep: Sterilization
- Dry Heat: Temperatures from 160-200°C, good for cylinders, open flasks, and beakers. Use a dry heat indicator.
- Autoclave: 15 psi, 121°C for 15 to 30 minutes for plastic or metal caps. Use autoclave tape and/or indicators.
Sterilization Methods
- Physical: Autoclaving (high temperatures), Dry heat.
- Chemical: Hydrogen peroxide gas.
Specimen Receiving, Processing, and Distribution
- Specimen Collection: Method of collection, labeling, submitter form (information regarding storage and preservation required), and shipment method.
- Receiving: Chain of custody, initial inspection (checking for cracks/leaks).
- Processing: Opening packages, labeling, sorting, initial preparation.
- Distribution: Secure carriers, organizing lab forms, delivering to appropriate labs.
Media Prep
- Types of media used, steps for preparation, accurate weighing of materials, homogeneous mixing, pH adjustments, sterilization and preventing overheating, labeling (media name, date made, and expiration date).
- Quality control of media: Inspecting and using only unexpired supplies.
Proper Decontamination
- Follow respective lab SOPs for decontamination.
- Appropriately decontaminate different lab materials.
- Use safe disposal practices according to regulations.
- Importance of waste segregation.
Quality of Laboratory Water
- Importance of quality water: Accuracy and reliability of results.
- Testing methods: Conductivity (measures ionic content), pH testing, bacterial count, and total organic carbon (TOC) tests.
- Testing frequency: Daily, monthly, or quarterly.
- Equipment: Use specific purity and pH meters, and filtration.
Conclusion
- Lab support services are crucial to a laboratory's success.
- Everything, from glassware to water quality, has an essential role in achieving reliable results.
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