Enzyme Biology Experiment PDF

Experiment Title Investigating the Effect of Temperature on Enzyme Activity (Catalase) Research Question How does temperature affect the activity of catalase in breaking down hydrogen peroxide? Hypothesis If the temperature increases up to an optimum level, the rate of reaction will increase. B...

Experiment Title Investigating the Effect of Temperature on Enzyme Activity (Catalase) Research Question How does temperature affect the activity of catalase in breaking down hydrogen peroxide? Hypothesis If the temperature increases up to an optimum level, the rate of reaction will increase. Beyond the optimum temperature, enzyme activity will decrease due to denaturation. Variables 1. Independent Variable: Temperature (e.g., 0°C, 20°C, 40°C, 60°C, 80°C). 2. Dependent Variable: Rate of reaction, measured by the volume of oxygen gas produced in a given time. 3. Controlled Variables: a. Concentration of hydrogen peroxide (e.g., 3% solution). b. Volume of hydrogen peroxide (e.g., 10 mL per trial). c. Source and quantity of catalase (e.g., 5g of blended potato or liver). d. Duration of reaction (e.g., 2 minutes per trial). Materials Hydrogen peroxide solution (3% concentration) Fresh potato or liver (as the source of catalase) Test tubes  Test tube rack Thermometer Water baths (to maintain specific temperatures) Stopwatch Graduated cylinder Measuring pipette Beaker Delivery tube and gas collection setup (e.g., an inverted measuring cylinder in a water trough) Method 1. Preparation: a. Blend the potato or liver with water to extract the catalase enzyme. Filter the mixture to remove large particles. b. Label the test tubes for different temperature conditions. 2. Temperature Adjustment: a. Use water baths to adjust the temperature of the hydrogen peroxide solution to the desired levels (e.g., 0°C, 20°C, 40°C, etc.). b. Allow the enzyme extract to reach the same temperature by placing it in the same water bath for a few minutes. 3. Reaction Setup: a. Add 10 mL of hydrogen peroxide to a test tube. b. Quickly add 5 mL of the enzyme solution and seal the test tube with a stopper attached to a delivery tube. c. Place the delivery tube in an inverted measuring cylinder filled with water to collect oxygen gas. 4. Timing: a. Start the stopwatch immediately after adding the enzyme solution. b. Measure the volume of oxygen gas collected after 2 minutes. 5. Repeat: a. Repeat the experiment three times for each temperature to ensure reliability. Data Collection Record the volume of oxygen gas produced at each temperature.  Create a table with temperature, volume of oxygen produced, and averages for multiple trials. Analysis Plot a graph of temperature (x-axis) vs. volume of oxygen produced (y-axis). Look for the temperature at which the enzyme activity is highest (optimum temperature). Identify the point where enzyme activity decreases due to denaturation. Conclusion Discuss whether the results support the hypothesis and explain the observed trends using scientific concepts (e.g., enzyme denaturation at high temperatures).