MLBT 1040 Hematology Unit 1 Objectives PDF

MLBT1040 Hematology Unit 1 Objectives ===================================== **TITLE: UNIT 1 -** Chapters 3, 4, 10 and 30 Procedures: Manual WBC, RBC and Platelet counts; Manual Hct, RBC Indices; and Coulter Labs **OBJECTIVES:** The student will be able to describe cellular structure and organiz...

MLBT1040 Hematology Unit 1 Objectives ===================================== **TITLE: UNIT 1 -** Chapters 3, 4, 10 and 30 Procedures: Manual WBC, RBC and Platelet counts; Manual Hct, RBC Indices; and Coulter Labs **OBJECTIVES:** The student will be able to describe cellular structure and organization; the processes for hematopoiesis and cellular maturation. The student will be able to perform manual WBC, RBC cell counts and calculate indices. The students will be able to describe the characteristics of hemoglobin and the hemoglobinopathies. **AFFECTIVE OBJECTIVES:** 1. Completes assignments on time 2. Demonstrates problem-solving skills 3. Displays skill in prioritizing and management of time and stress **COGNITIVE OBJECTIVES**: Chapter 3 1. Describe the chemical composition and general function of cellular membranes 2. Explain the general membrane activities of passive and facilitated diffusion, active transport, osmosis and endocytosis. 3. Describe the structure and function of each of the cytoplasmic organelles, cell membrane and nucleus. 4. Define the term apoptosis and compare active and passive cell death. 5. Compare the characteristics of tissue necrosis and apoptosis. 6. Describe the processes of mitosis and meiosis. 7. Define the terms gene and alleles and discuss genetic alterations including hemophilia and sickle cell anemia. 8. Explain the impact of oncogenes and give an example of a genetic alteration. 9. Describe the functions of protooncogenes. Chapter 4 1. Name the sites of hematopoiesis, including erythropoiesis, from early embryonic stage of development until fully established in adults. 2. Compare the developmental events of embryonic, hepatic and early medullary phases of hematopoiesis. 3. Name and compare the organs and tissues of the hematopoietic system. 4. Define and identify sites of extramedullary hematopoiesis. 5. Describe conditions that produce hepatosplenomegaly 6. Describe the characteristics and functions of the thymus, spleen and lymph nodes. 7. Contrast the features of erythropoiesis, granulopoiesis, lymphopoiesis and megakaryopoiesis. 8. List the hematopoietic growth factors. 9. Name the cells in developmental order that will mature into erythrocytes, thrombocytes, plasma cells and the five leukocyte types 10. Compare the nuclear characteristics of **shape, chromatin pattern and nucleoli in cell types** 11. Compare the cytoplasmic features of color, granulation, shape, quantity, vacuolization and inclusions to cell maturity. Chapter 10 1. List and define the components of a complete blood count (CBC) 2. Calculate a manual RBC cell count when given the dilution and numbers of cells counted 3. Describe the principles of the cyanmethemoglobin assay for determination of hemoglobin. 4. Describe the measurements of a microhematocrit. 5. Compare RBC, hemoglobin and hematocrit values using the rule of three 6. Define, calculate and classify RBC morphology for each of the RBC indices: MCV, MCH and MCHC. 7. Compare the morphological appearances of reticulocytes stained with Wright stained and supravital stain such as new methylene blue 8. Calculate a manual WBC cell count given the dilution and numbers of cells counted. 9. Name the conditions that can produce leukocytosis and those that produce leukocytopenia. 10. Calculate an absolute cell count. 11. Discuss the normal values for RBC, WBC, Hct, Hgb, MCV, MCH, MCHC and Reticulocytes Chapter 30 1. Describe the basic theory of the following principles of cell counting and sizing: 1) Electrical impedance and 2 ) Optical detection 2. Explain the fundamental concepts of laser technology 3. Describe the principles of flow-cell cytometry and two basic uses in hematology. 4. List the parameters measured and the methods used to measure these paraments in basic benchtop hematology analyzers 5. Describe the process and output of total cell and histogram electrical impedance systems 6. Describe the process and output of a laser scatter technology system. 7. Describe the appearance of microcytic and macrocytic rbc's on a histogram 8. Define RDW, how it is calculated, the normal range and how it is relations to MCV 9. Describe the appearance of a leukocyte histogram by both electrical and optical detection methods. 10. Describe the construction of a platelet histogram. 11. Define MPV, its relationship with platelets and four disorders associated with it. 12. Describe the generation, by laser technology, of a histogram of: RBC, WBC and Platelets 13. Describe the general functions that flow cytometry can provide and the hematological applications. 14. Interpret histogram and scatter plot data **PSYCHOMOTOR OBJECTIVES:** - **Students are given [2 attempts to achieve a 70% or higher] on the Lab Skills Test.** - If after 2 attempts, the student is unable to achieve a passing grade, the student will be dismissed from the course and program. - NOTE: If a student [misses a lab test], they will [receive a zero and it will count as their 1^st^ attempt]. They will only be given 1 additional lab attempt to complete with a 70% or higher grade. **RESOURCES:** [Textbook and Handouts] **LEARNING ACTIVITIES:** 1. READ Chapters 3, 4, 10, and 30 2. Perform Manual procedures: WBC, RBC, and Platelet counts; RBC Indices 3. Attend all class lectures 4. Perform all laboratories **EVALUATION:** Information from Unit I will be included on Test \#1 and Laboratory Test \#1