Lecture+5_Signal+Transduction+Part+2.pptx

Optional accompanying reading Pharmacology 4001: Mechanisms of Drug Action Chapter 15 Lecture 4: Signal Transduction, Part II January 23, 2024 Signal Transduction, Part II Figure 15.20 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 200...

Optional accompanying reading Pharmacology 4001: Mechanisms of Drug Action Chapter 15 Lecture 4: Signal Transduction, Part II January 23, 2024 Signal Transduction, Part II Figure 15.20 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002, Garland Science Optional accompanying reading: Chapter 15 Second Messengers Review: A) In one sentence, describe two properties of a second messenger B) Name two second messengers Complex Interactions ure 2.17 of M Ptashne and A Gann “Genes and Signals” 2002, Cold Spring Harbor Laboratory Press Intracellular and Extracellular Receptors Figure 15.4 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002,.Garland Science Nuclear Receptor Signaling Molecules Figure 15.12 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002,.Garland Science Nuclear Receptor: Regulated Recruitment Figure 15.13 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002,.Garland Science Enzyme-linked Receptors Note that there is a distinction between tyrosine kinaselinked receptors and tyrosine kinases Figure 15.15 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002,.Garland Science G protein-coupled Receptors Figure 15.28 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002, Garland Science gure 15.28 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter Molecular Biology of the Cell” fourth edition 2002, Garland Science Phospholipase C: Stimulated by Gq Figure 15.36 of B Alberts, A Johnson, J Lewis, M Raff, K Roberts and P Walter “Molecular Biology of the Cell” fourth edition 2002, Garland Science Learning Objectives Revisited 1. Name the simplified components of a signaling pathway. 1. 2. 3. 4. Signal molecule (first messenger) Receptor protein Intracellular signaling proteins (second messengers or kinases) Target proteins 2. List and describe the different types of signaling. 1. 2. 3. 4. 5. Endocrine signaling depends on endocrine cells, which secrete hormones into the bloodstream for distribution throughout the body. Paracrine signaling depends on local mediators that are released into the extracellular space and act on neighboring cells. Autocrine signaling occurs when a cell secretes a signaling molecule that binds back to its own receptors. Contact-dependent signaling requires cells to be in direct membrane–membrane contact. Synaptic signaling is performed by neurons that transmit signals electrically along their axons and release neurotransmitters at synapses 3. Describe the characteristics of second messengers. Small molecules Rapidly generated Generated from readily available sources (e.g., ion gradients, plasma membrane phospholipids) Readily reversible (i.e., can be turned off quickly by phosphodiesterases or ion transporters. 4. How are second messengers generated and inactivated? They are generated from readily available sources, including bembrane sources (IP3, DAG), nucleotide sources (cAMP, cGMP), ion gradients (Ca+2, Na+, K+), They are inactivated by phosphodiesterases (cAMP, cGMP), ion transporters (Ca+2, Na+, K+), enzymatic inactivation (prostaglandins, IP3, diacylglycerol) Homework Assignments for Jan 16 – Feb. 13 Complete Quiz #5 by Thursday at 9:05 am. On Canvas. Over Review Article. Download REVIEW ARTICLE #1: Hanahan, D., and R. A. Weinberg (2000). The hallmarks of cancer. Cell 100, 57-70. – Start reading and prepare to discuss during lecture on Feb. 1 Download RESEARCH PAPER #1: Druker at al. (1996). Effects of a specific inhibitor of the Abl tyrosine kinase on growth of Bcr-Abl positive cells. Nature Medicine 2, 561-564. – Start reading and prepare to discuss during lecture on Feb. 13