Connective Tissue Lecture Notes PDF

Connective Tissue Connective Tissue Proper Yomna Fathy Hassan Lecturer of Medical Histology & Cell Biology Faculty of Medicine-Mansoura University  Connective Tissue Definition The connective tissue supports and connects various tissues and organs. Types...

Connective Tissue Connective Tissue Proper Yomna Fathy Hassan Lecturer of Medical Histology & Cell Biology Faculty of Medicine-Mansoura University  Connective Tissue Definition The connective tissue supports and connects various tissues and organs. Types Connective tissue proper Soft in consistency Connective Tissue Cartilage Rubbery in consistency Bone Hard in consistency  Connective Tissue Proper Fibers Cells Matrix 1-White collagenous fibers Rounded or oval Branched C. T. cells C. T. cells 2-Yellow elastic fibers 1 Plasma cells. 1 Undifferentiated 3-Reticular fibers 2 Mast cells. mesenchymal cells. 2 Fibroblasts and fibrocytes. 3 Adipose cells. 4 Blood 3-Macrophages. leucocytes. 4-Pigment cells.  Connective Tissue Fibers White Collagenous Fibers Characters -Color: White in color. -Strong and resist stretch. -Wavy bundles branch but the single fiber does not branch. L/M -H & E → Acidophilic. -Mallory trichrome stain → Blue. Sites 1. Type I collagen: bone, teeth and tendon. 2. Type II collagen: hyaline and elastic cartilage. Yellow Elastic Fibers Characters -Color: Yellow. -Elastic in nature. -The fibers branch and may form elastic membranes, e.g. in the wall of the aorta. L/M - H & E → Acidophilic. - Van Gieson's stain → Yellow. - Verhoeff's stain → Black. Sites -Walls of arteries. Reticular Fibers Characters They are very thin fibers that branch and anastomose to form a network. L/M - H & E → Not stained. - Silver → Black. Sites Stroma of parenchymatous organs e.g. Liver, spleen and lymph nodes.  White Collagenous Fibers Yellow Elastic Fibers Reticular Fibers Characters -Color: White in color. -Color: Yellow. They are very thin -Strong and resist stretch. -Elastic in nature. fibers that branch and -Wavy bundles branch but the -The fibers branch and may form anastomose to form a single fiber does not branch. elastic membranes, e.g. in the wall of network. the aorta. L/M -H & E → Acidophilic. - H & E → Acidophilic. - H & E → Not stained. -Mallory trichrome stain → - Van Gieson's stain → Yellow. - Silver → Black. Blue. - Verhoeff's stain → Black. Sites 1. Type I collagen: bone, teeth -Walls of arteries. Stroma of and tendon. parenchymatous organs: 2. Type II collagen: hyaline Liver, spleen and lymph and elastic cartilage. nodes. Which of the followings is a site for type I collagen fibers? a)Bone. b) Hyaline cartilage. c) Elastic cartilage. d) Stroma of liver. e) Stroma of lymph nodes Which of the followings is a special stain for elastic fibers? a) Mallory trichrome stain. b) Van Gieson's stain. c) Silver stain. d) Sudan black stain. e) Best’s carmine stain.  Connective Tissue Proper Fibers Cells Matrix 1 White collagenous fibers Rounded or oval Branched C. T. cells C. T. cells 2 Yellow elastic fibers 1 Plasma cells. 1 Undifferentiated 3-Reticular fibers mesenchymal cells. 2 Mast cells. 2 Fibroblasts and fibrocytes. 3 Adipose cells. 4 Blood 3-Macrophages. leucocytes. 4-Pigment cells.  Connective Tissue Cells Rounded or oval Branched C. T. cells C. T. cells -Plasma cells. -Undifferentiated mesenchymal cells. -Mast cells. -Fibroblasts and fibrocytes. -Adipose cells. -Macrophages. -Blood leucocytes. -Pigment cells.  Rounded or oval C. T. cells Plasma cells L/M Nucleus Cart-wheel in shape due to special arrangement of its chromatin. Cytoplasm Deep basophilic with negative Golgi image. E/M The cytoplasm is rich in ribosomes, rER and Golgi apparatus. Function Secretion of antibodies. Mast cells L/M Cytoplasm Contains large basophilic granules. Special stains Can be stained metachromatically: Toluidine blue: Reddish purple color. E/M The cytoplasm contains electron dense granules. Function Synthesis, storage and secretion of histamine in cases of inflammation and allergy.  Adipose cells White adipose cells Brown adipose cells L/M Nucleus Eccentric flattened (signet- Central rounded. ring). Special stains: - Sudan III: Orange. - Sudan black: Black. - Osmic acid: Black. E/M - Single large fat globule. -Numerous small fat droplets. - Few cell organelles around -Numerous mitochondria. the nucleus. Function 1 Storage of extra energy. -Rapid heat production on exposure to 2 Heat insulation. cold. Blood leucocytes Leave the blood stream and appear in the C.T. in certain conditions, e.g.: Eosinophils → in allergy. Neutrophils, monocytes & lymphocytes → in infections. Which of the following connective tissue cells have large metachromatic granules in the cytoplasm? a)Plasma cells. b) Mast cells. c) White adipose cells. d)Macrophages. e)Undifferentiated mesenchymal cells.