Histotechnologist G3 PDF

HISTOTECHNOLOGIST Prepared by Group 3 Histotechnologist (“Tissue Processor”) The work of the histologic technician (HT) and histotechnologist (HTL) is somewhat analogous to the clinical laboratory technician (CLT), and clinical laboratory scientist (CLS). Histotechnologist (“Tissue...

HISTOTECHNOLOGIST Prepared by Group 3 Histotechnologist (“Tissue Processor”) The work of the histologic technician (HT) and histotechnologist (HTL) is somewhat analogous to the clinical laboratory technician (CLT), and clinical laboratory scientist (CLS). Histotechnologist (“Tissue Processor”) Histologic technicians or histotechnologists routinely prepare, process, and stain biopsies and tissue specimens for microscopic examination by a pathologist. Histopathology Autopsy Specimens taken from the morgue Also known as Post-mortem mortem Examination or Necropsy. Biopsy Specimens collected from the operating room.  Routine and Special Tests in Histopathology Immunohistochemical Routine Histopathologic Pap Smear Examination staining Papanicolaou (PAP) smear is a Immunohistochemical staining is a method of screening for process of detecting antigens in the cells of tissue sections by using the cervical cancer and pre- principle of antibodies binding to cancerous changes in th cevix. specific antigens.  Routine Histopathologic Techniques 1. Numbering 5. Infiltration 9. Staining 6. Embedding/ 2. Fixation 10. Mounting Molding 3. Dehydration 7. Trimming 11. Labeling 4. Clearing 8. Sectioning  Routine Histopathologic Technique 1. Numbering 2. Fixation 3. Dehydration 4. Clearing The process of Preserving the Process of Also known as de- recording the tissue tissue specimen removing water alcoholization. specimen in a logbook from the 10% formalin specimen by Xylene and assigning routine fixative using increasing routine clearing identification numbers used in the grades of ethyl agent employed to specimen recieved. alcohol. in the laboratory. laboratory.  Routine Histopathologic Technique 5. Infiltration 6. Embedding/ 7. Trimming 8. Sectioning The process of filling up Molding The process of removing Also known as microtomy. tissue spaces or cavities the excess paraffin wax In sectioning, a tissue block The process of placing from the block until it using melted paraffin is cut into thin slices called wax. the inflitrated tissue assumes tue shape of a ribbons/section using a inside a mold. truncated pyramid. microtome.  Routine Histopathologic Technique 9. Staining 10. Mounting 11. Labeling 8. Sectioning The process that The process of putting Is a means of identifying Note: Mislabeling is the employs the use of the specimen. In hematoxylin and the cover slip on the labeling, the specimen mortal sin in the eosin dyes to stained tissue using a number is indicated in laboratory! differentiate the cells mounting medium. the glass slides. and cellular constituents.  THANK YOU Reference: Introduction to Medical Technology Practice in the Philippine Setting (Cardona, et al. 2015)