Podcast
Questions and Answers
What is the primary role of a histotechnologist?
What is the primary role of a histotechnologist?
- To analyze blood samples
- To manufacture laboratory equipment
- To prepare and process tissue specimens for microscopic examination (correct)
- To perform surgeries on patients
Which technique is used for screening cervical cancer?
Which technique is used for screening cervical cancer?
- Immunohistochemical staining
- Pap smear (correct)
- Autopsy examination
- Routine Biopsy processing
What is the purpose of the fixation process in histopathology?
What is the purpose of the fixation process in histopathology?
- To dehydrate the tissue specimen
- To preserve the tissue specimen (correct)
- To stain the tissue specimen
- To remove water from the specimen
Which of the following is a step involved in the routine histopathologic technique?
Which of the following is a step involved in the routine histopathologic technique?
What is dehydration in the context of histopathology?
What is dehydration in the context of histopathology?
Which of the following terms refers to the examination of specimens taken from the morgue?
Which of the following terms refers to the examination of specimens taken from the morgue?
What does immunohistochemical staining detect?
What does immunohistochemical staining detect?
What is meant by the clearing process in histopathology?
What is meant by the clearing process in histopathology?
What is the primary purpose of infiltration in histopathological techniques?
What is the primary purpose of infiltration in histopathological techniques?
Which staining technique employs hematoxylin and eosin dyes?
Which staining technique employs hematoxylin and eosin dyes?
What is the process of embedding in histopathology?
What is the process of embedding in histopathology?
What is the purpose of sectioning in histopathology?
What is the purpose of sectioning in histopathology?
What could result from mislabeling specimens in the laboratory?
What could result from mislabeling specimens in the laboratory?
What is the significance of mounting in histopathology?
What is the significance of mounting in histopathology?
What is meant by labeling in histopathologic techniques?
What is meant by labeling in histopathologic techniques?
During the clearing process in histopathology, what agent is primarily used?
During the clearing process in histopathology, what agent is primarily used?
Flashcards
Histotechnologist
Histotechnologist
A medical professional who prepares and processes tissue samples for microscopic examination by a pathologist.
Biopsy
Biopsy
A tissue sample taken from a living patient during surgery or another procedure.
Autopsy Specimens
Autopsy Specimens
Tissue samples taken during a post-mortem examination of a deceased individual.
Routine Histopathologic Examination
Routine Histopathologic Examination
Signup and view all the flashcards
Immunohistochemical Staining
Immunohistochemical Staining
Signup and view all the flashcards
Dehydration
Dehydration
Signup and view all the flashcards
Fixation
Fixation
Signup and view all the flashcards
Clearing
Clearing
Signup and view all the flashcards
Routine Histopathologic Technique
Routine Histopathologic Technique
Signup and view all the flashcards
Specimen Identification Number
Specimen Identification Number
Signup and view all the flashcards
Formalin Fixation
Formalin Fixation
Signup and view all the flashcards
Xylene Clearing
Xylene Clearing
Signup and view all the flashcards
Paraffin Wax Infiltration
Paraffin Wax Infiltration
Signup and view all the flashcards
Tissue Embedding
Tissue Embedding
Signup and view all the flashcards
Tissue Sectioning (Microtomy)
Tissue Sectioning (Microtomy)
Signup and view all the flashcards
Staining (Hematoxylin & Eosin)
Staining (Hematoxylin & Eosin)
Signup and view all the flashcards
Study Notes
Histotechnologist Overview
- Histotechnologists (HTs) and histologic technicians (HTLs) prepare, process, and stain tissue samples for microscopic examination by pathologists.
- Their work is similar to clinical laboratory technicians (CLTs) and clinical laboratory scientists (CLSs).
Histopathology
- Histopathology examines tissue samples to diagnose diseases.
- Autopsy: Examination of tissue from a deceased person's body, also known as post-mortem or necropsy examination.
- Biopsy: Examination of tissue samples removed from a living person during surgery, to determine the cause of problems with organs or tissue.
Routine and Special Tests in Histopathology
- Pap Smear: A screening method for cervical cancer and precancerous changes in the cervix.
- Immunohistochemical staining detects antigens in tissue cells using antibodies.
Routine Histopathologic Techniques
- 1. Numbering: Recording tissue samples.
- 2. Fixation: Preserving tissue samples using 10% formalin.
- 3. Dehydration: Removing water from samples using increasing concentrations of alcohol.
- 4. Clearing: Removing alcohol using xylene.
- 5. Infiltration: Filling tissue spaces with melted paraffin wax.
- 6. Embedding/Molding: Placing infiltrated tissue in molds.
- 7. Trimming: Removing excess paraffin from the tissue block.
- 8. Sectioning: Cutting tissue into thin slices using a microtome.
- 9. Staining: Employing hematoxylin and eosin dyes to differentiate cells and tissue structures.
- 10. Mounting: Applying a mounting medium to tissue sections before adding cover slips.
- 11. Labeling: Identifying the specimen with a number on the slide.
Additional Notes
- Mislabeling is a critical error in the lab.
- Tissue processing can be performed quickly, in some cases within minutes.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
