Hematology 2 Lab Module 1 3D Notes PDF

HEMATOLOGY 2 LABORATORY: MODULE 1 – Special Laboratory Methods for Leukocytes MA’AM PARREÑAS, RMT & MA’AM TINASAS, RMT * To whom it may concern: If you have received a copy of this, please do not edit NOR remove anything found on this file. You will NOT PASS if...

HEMATOLOGY 2 LABORATORY: MODULE 1 – Special Laboratory Methods for Leukocytes MA’AM PARREÑAS, RMT & MA’AM TINASAS, RMT * To whom it may concern: If you have received a copy of this, please do not edit NOR remove anything found on this file. You will NOT PASS if you dare to do so. Respect lang po! Salamat! Absolute Eosinophil Count PROCEDURE !! Eosinophils are high in terms of respiratory diseases (ex. Pulmonary 1. Draw well-mixed anticoagulated whole blood to the 1 mark of the infiltrates, Hay Fever) & allergic reactions (alongside basophils) WBC pipette. 2. Wipe the outside portion of the pipette and draw the diluting fluid to PRINCIPLE the 11 mark. Eosinophils are identified and counted microscopically after the 3. Mix for 10 minutes using a pipette shaker. specimen is diluted and loaded on a hemocytometer. 4. Expel the first 4 drops and fill both sides of the counting chamber. 5. Allow the cells to settle for at least 3 minutes before counting. 6. Count the eosinophils in the entire ruled area of both sides of the In the diluting fluid; hemocytometer (9 large squares). 7. Get the average count and compute for the absolute count. Phloxine stains eosinophils red Propylene glycol lyses the RBCs COMPUTATION Heparin inhibits leukocyte clumping Sodium carbonate lyses all WBCs except eosinophils 100 = DF / 0.1mm depth REFERENCE RANGE 150 - 300 / mm3 of blood Table 1.1: Diluting fluids with phloxine Phloxine Manner’s Randolph’s INTERPRETATION Pilot’s solution dluting fluid diluting fluid diluting fluid Eosinopenia — hyperadrenalism or Cushing’s syndrome Phloxine Phloxine Phloxine Phloxine Eosinophilia o Allergic reactions Propylene glycol Propylene glycol Urea Propylene glycol CAP o Parasitic infections Sodium Sodium o Certain leukemias Trisodium citrate Calcium chloride carbonate carbonate Distilled water Distilled water Distilled water THORN’S TEST (Eosinophil Depression Test) A test for normal adrenal function Heparin Patient is on fasting state. — Baseline eosinophil count is determined. Patient is given ACTH injection. — Eosinophil count after 4 hrs is determined. CAYANAN, ESPADA, GUEVARRA, SALINAS 1  HEMATOLOGY 2 LABORATORY: MODULE 1 – Special Laboratory Methods for Leukocytes MA’AM PARREÑAS, RMT & MA’AM TINASAS, RMT * To whom it may concern: If you have received a copy of this, please do not edit NOR remove anything found on this file. You will NOT PASS if you dare to do so. Respect lang po! Salamat! INTERPRETATION When the Neutrophils are incubated with colourless soluble dye The second absolute count is 50% (Nitroblue Tetrazolium), reduction produces blue-black formazan NORMAL lower than the fasting count. precipitates, the number of neutrophils that reduced the dye is HYPOADRENALISM quantified. Increase in eosinophil count !! Heparinized blood or buffy coat is used (Addison’s disease) HYPERADRENALISM Decrease in eosinophil count (Cushing’s disease) INTERPRETATION !! Eosinophils decrease in presence of cortisol due to increased apoptosis >10 NBT- positive neutrophils – Systemic bacterial infection