Mixed Noncompetitive Inhibition PDF
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This document explains mixed noncompetitive inhibition, a type of enzyme inhibition. It details the behavior of proteins and enzymes, and how to understand the Lineweaver-Burk plot of mixed noncompetitive inhibition. This document is a great resource for students who are studying biochemistry.
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Chapter 6 The Behavior of Proteins: Enzymes Mixed Noncompetitive Inhibition © 2018 Cengage...
Chapter 6 The Behavior of Proteins: Enzymes Mixed Noncompetitive Inhibition © 2018 Cengage Learning. All Rights Reserved. Mixed Noncompetitive Inhibition Similar to noncompetitive inhibition but binding of Inhibitor DOES affect binding of S and vice versa Dissociation constants KI and K′I not identical Still lowers Vmax © 2018 Cengage Learning. All Rights Reserved. Lineweaver–Burk Plot for Noncompetitive Inhibition Slope and y intercept change x intercept remains unchanged Noncompetitive inhibition 1 K = M I 1 1 I 1 + × + 1 + V Vmax K I S Vmax K I y = m × x + b © 2018 Cengage Learning. All Rights Reserved. Identifying a Noncompetitive Inhibitor Lineweaver-Burk equation: 1 KM 1 1 = × + V Vmax S Vmax Substitute modified value of Vmax: ★ ★ © 2018 Cengage Learning. All Rights Reserved. Lineweaver–Burk Plot for Mixed Noncompetitive Inhibition Slope and y intercept change x intercept changes Noncompetitive inhibition 1 K = M I 1 1 I 1 + × + 1 + V Vmax K I S Vmax K I y = m × x + b © 2018 Cengage Learning. All Rights Reserved. Mixed Noncompetitive Inhibition Vmax decreases KM increases (or decreases) © 2018 Cengage Learning. All Rights Reserved.
