Mixed Noncompetitive Inhibition PDF

Summary

This document explains mixed noncompetitive inhibition, a type of enzyme inhibition. It details the behavior of proteins and enzymes, and how to understand the Lineweaver-Burk plot of mixed noncompetitive inhibition. This document is a great resource for students who are studying biochemistry.

Full Transcript

Chapter 6 The Behavior of Proteins: Enzymes Mixed Noncompetitive Inhibition © 2018 Cengage...

Chapter 6 The Behavior of Proteins: Enzymes Mixed Noncompetitive Inhibition © 2018 Cengage Learning. All Rights Reserved. Mixed Noncompetitive Inhibition Similar to noncompetitive inhibition but binding of Inhibitor DOES affect binding of S and vice versa Dissociation constants KI and K′I not identical Still lowers Vmax © 2018 Cengage Learning. All Rights Reserved. Lineweaver–Burk Plot for Noncompetitive Inhibition Slope and y intercept change x intercept remains unchanged Noncompetitive inhibition 1 K = M   I  1 1   I  1 +  × + 1 +  V Vmax  K I   S  Vmax  K I  y = m × x + b © 2018 Cengage Learning. All Rights Reserved. Identifying a Noncompetitive Inhibitor Lineweaver-Burk equation: 1 KM 1 1 = × + V Vmax  S Vmax Substitute modified value of Vmax: ★ ★ © 2018 Cengage Learning. All Rights Reserved. Lineweaver–Burk Plot for Mixed Noncompetitive Inhibition Slope and y intercept change x intercept changes Noncompetitive inhibition 1 K = M   I  1 1   I  1 +  × + 1 +  V Vmax  K I   S  Vmax  K I  y = m × x + b © 2018 Cengage Learning. All Rights Reserved. Mixed Noncompetitive Inhibition Vmax decreases KM increases (or decreases) © 2018 Cengage Learning. All Rights Reserved.

Use Quizgecko on...
Browser
Browser