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Questions and Answers
What is a primary characteristic of mixed noncompetitive inhibition?
What is a primary characteristic of mixed noncompetitive inhibition?
- It does not affect the binding of substrate.
- It affects the binding of substrate and inhibitor mutually. (correct)
- The dissociation constants KI and K′I are identical.
- It decreases both Vmax and KM.
In a Lineweaver–Burk plot for mixed noncompetitive inhibition, which of the following changes?
In a Lineweaver–Burk plot for mixed noncompetitive inhibition, which of the following changes?
- The slope and y-intercept remain unchanged.
- Both the slope and y-intercept change. (correct)
- The y-intercept changes while the x-intercept changes. (correct)
- The x-intercept remains unchanged.
What happens to Vmax in the case of mixed noncompetitive inhibition?
What happens to Vmax in the case of mixed noncompetitive inhibition?
- Vmax decreases. (correct)
- Vmax is independent of inhibitor concentration.
- Vmax increases.
- Vmax remains constant.
How does KM behave during mixed noncompetitive inhibition?
How does KM behave during mixed noncompetitive inhibition?
Which of the following equations correctly represents the Lineweaver–Burk equation for mixed noncompetitive inhibition?
Which of the following equations correctly represents the Lineweaver–Burk equation for mixed noncompetitive inhibition?
What is the impact of a mixed noncompetitive inhibitor on the relationship between substrate concentration and reaction rate?
What is the impact of a mixed noncompetitive inhibitor on the relationship between substrate concentration and reaction rate?
Which statement correctly describes the dissociation constants in mixed noncompetitive inhibition?
Which statement correctly describes the dissociation constants in mixed noncompetitive inhibition?
How does mixed noncompetitive inhibition affect the Michaelis-Menten constant (KM)?
How does mixed noncompetitive inhibition affect the Michaelis-Menten constant (KM)?
What changes occur to the x-intercept in a Lineweaver–Burk plot for mixed noncompetitive inhibition?
What changes occur to the x-intercept in a Lineweaver–Burk plot for mixed noncompetitive inhibition?
What is the effect of mixed noncompetitive inhibition on Vmax?
What is the effect of mixed noncompetitive inhibition on Vmax?
In the context of mixed noncompetitive inhibition, what does the term 'binding of inhibitor' refer to?
In the context of mixed noncompetitive inhibition, what does the term 'binding of inhibitor' refer to?
Which of the following can be observed in a Lineweaver–Burk plot of mixed noncompetitive inhibition?
Which of the following can be observed in a Lineweaver–Burk plot of mixed noncompetitive inhibition?
What does the Lineweaver-Burk equation indicate about the relationship between reaction rate and substrate concentration?
What does the Lineweaver-Burk equation indicate about the relationship between reaction rate and substrate concentration?
In terms of the behavior of enzymes, how does mixed noncompetitive inhibition differ from traditional noncompetitive inhibition?
In terms of the behavior of enzymes, how does mixed noncompetitive inhibition differ from traditional noncompetitive inhibition?
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Study Notes
Mixed Noncompetitive Inhibition
- Similar to noncompetitive inhibition, but the inhibitor binding affects substrate binding and vice versa.
- Dissociation constants KI and K′I are not identical.
- Vmax is still lowered.
Lineweaver-Burk Plot for Noncompetitive Inhibition
- Slope and y-intercept change.
- x-intercept remains unchanged.
Lineweaver-Burk Plot for Mixed Noncompetitive Inhibition
- Slope and y-intercept change.
- x-intercept changes.
Mixed Noncompetitive Inhibition
- Vmax decreases.
- KM increases or decreases.
Mixed Noncompetitive Inhibition
- Similar to noncompetitive inhibition, but differs in that the inhibitor affects the binding of the substrate and vice versa.
- The dissociation constants for the inhibitor, KI and KI', are not identical.
- Mixed noncompetitive inhibition still lowers the maximum velocity (Vmax) of the reaction.
Lineweaver–Burk Plot for Mixed Noncompetitive Inhibition
- The slope and y-intercept of the Lineweaver-Burk plot change, while the x-intercept also changes.
- The equation for the Lineweaver-Burk plot under mixed noncompetitive inhibition is: 1/V = (1 + [I]/KI')/Vmax * 1/[S] + (1 + [I]/KI)/Vmax.
Identifying a Mixed Noncompetitive Inhibitor
- The Lineweaver-Burk equation for the uninhibited reaction is: 1/V = KM/Vmax * 1/[S] + 1/Vmax.
- Substitute the modified value of Vmax from the mixed noncompetitive inhibition equation into the Lineweaver-Burk equation to identify a mixed noncompetitive inhibitor.
Mixed Noncompetitive Inhibition Effects
- Vmax decreases.
- KM may increase or decrease.
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