BSc(Hons) Horticulture Past Paper 2023-2024 PDF

# MAHARASHTRA AGRICULTURAL UNIVERSITIES EXAMINATION BOARD, PUNE ## SEMESTER END THEORY EXAMINATION ### B.Sc.(Hons.) Horticulture - Semester: III (New) - Course No.: II/BIOT 231 - Credits: 2 (1+1) - Term: First - Academic Year: 2023-24 - Title: Elementary Plant Biotechnology - Day & Date: Tuesday,...

# MAHARASHTRA AGRICULTURAL UNIVERSITIES EXAMINATION BOARD, PUNE ## SEMESTER END THEORY EXAMINATION ### B.Sc.(Hons.) Horticulture - Semester: III (New) - Course No.: II/BIOT 231 - Credits: 2 (1+1) - Term: First - Academic Year: 2023-24 - Title: Elementary Plant Biotechnology - Day & Date: Tuesday, 12.12.2023 - Time: 9:00 to 11:00 hrs. - Total Marks: 40 **Note:** 1. Solve ANY EIGHT questions from SECTION 'A'. 2. All questions from SECTION 'B' are compulsory. 3. All questions carry equal marks. 4. Draw neat diagram wherever necessary. ## SECTION 'A' **Q.1** Define Molecular marker. Enlist various types of molecular marker. State their applications for crop improvement. **Q.2** Define Somaclonal variations. What are causes/reasons of induction of somaclonal variation in plants? **Q.3** a) Define Nutrient media. Name three antioxidants and three antibiotics used in media. b) Define Totipotency and explain it with reference to dedifferentiation and redifferentiation. **Q.4** a) Define Micropropagation. State the advantages of micropropagation. b) Write in detail about three steps of PCR technique. **Q.5** Define Blotting. Enlist the types of blotting. Distinguish between Southern and Northern blotting techniques. **Q.6** Define DNA fingerprinting. Write in detail the procedure of DNA fingerprinting. **Q.7** a) Describe two types of somatic embryogenesis. Write its applications. b) Write precisely about protoplast fusion by spontaneous fusion method and induced fusion method. **Q.8** Define Plant Biotechnology. Discuss at length about importance of Biotechnology in Horticulture. **Q.9** a) What is Test tube fertilization? State its applications? b) Define Genetic Engineering. Enlist vector-mediated and vector-less methods of gene transfer. **Q.10** Write short notes on (Any Two): a) Three types of nanomaterial with examples of Nano-biotechnology b) Applications of marker-assisted selection in crop improvement c) Synthetic/Artificial seed ## SECTION ‘B’ **Q.11** Match the pairs: | 'A' | 'B' | |---|---| | Meristem culture | Crown gall disease | | Agrobacterium tumefaciens | Callus | | Thermus aquaticus | Virus-free plants of Dahlia | | Habituation | DNA polymerase | **Q.12** State True or False: 1) Letham derived kinetin-like substance from Maize endosperm and named it Picloram. 2) Glossy and glassy appearance of tissue cultured plants is called ex vitro. 3) EDTA is not a chelating agent. 4) Tissue cultured plants within in vitro conditions are autotrophic in nature.