Bacterial Pathogenesis (Lecture 1) PDF

Bacterial pathogenesis  Pathogen: microbe has ability to causing infection, some microbes are highly pathogenic, whereas others cause disease rarely (low pathogenic).  Virulence: it is referred to degree of ability of microbe to produce disease. The viru...

Bacterial pathogenesis  Pathogen: microbe has ability to causing infection, some microbes are highly pathogenic, whereas others cause disease rarely (low pathogenic).  Virulence: it is referred to degree of ability of microbe to produce disease. The virulence is determined by virulence factors such as capsule enzymes and toxins.  Infection, in general, is presence and multiplication of microbe within human body, and cause infectious disease.  Characters of pathogen: 1. Pathogenic organism should be able to enter the host body. 2. Pathogenic organism should be able to multiply in tissue. 3. Pathogenic organism should be able to damage the tissue. 4. They must be capable to resist the host defense. Virulence factors of pathogen: Some important virulence factors that have role in pathogenesis of bacteria: 1. Capsule: 1   Which mediate adherence of pathogen to specific receptors on host cell surface.  Prevent the phagocytes from adhering to bacteria, therefore act as antiphagocytic factor. 2. Pili:  Which mediate adherence to specific sites on surface of host cell. 3. Enzymes:  Several enzymes secreted by bacteria play role in pathogenesis. 4. Toxins  The bacteria produce two types of toxins; exotoxin and endotoxin. Sources of infection: 1. Human: is common source of infection from patients or carriers. Fomites are inanimate objects of patients that serve as source of organism. 2. Animals; are important source of organisms that infect human, the organisms can transmitted from infected animals to human and cause disease. The disease is called zoonosis. 3. Soil; may serves as source of infection for many pathogenic organisms or spores of some bacteria and fungi. 4. Food; contaminated food may be source of infection, eg. Staphylococcal food poisoning. 5. Water; many pathogenic MO such as Vibrio cholerae, hepatitis virus may be found in water. Stages of bacterial pathogenesis: I. Transmission and portal of entry: 2  A- Portals of entry (routes of infection): four important portals of entry 1. Respiratory tract. 2. Elementary tract. 3. Skin 4. Genital tract. B- Mode of transmission 1. Inhalation: the bacteria may be transmitted by inhalation of respiratory secretions of infected patients or by inhalation of contaminated dust with bacteria (from soil). 2. Ingestion: the bacteria can be transmitted by consumption of contaminated water or food. 3. Skin contact, bite, wound: The transmission may occur through skin contact or can be occurs by trauma. The transmission from animal to human can take place either directly from bite of reservoir animal host; or indirectly through the bite of insect vectors. Those bacteria may be introduced directly into blood stream or remain at site of infection. 4. Blood transfusion: some bacteria can be transmitted by blood transfusion. Injection of needles during intravenous drug use or indwelling catheter, have role in transmission. 5. Sexual intercourse: certain bacteria can be transmitted by sexual contact. All above methods are called horizontal transmission. 6. Transplacental; the infection of fetus can occur between mother and offspring across the placenta, at time of delivery, or during breast feeding. The transmission from mother to her fetus is called Vertical transmission. 3 II. Adherence and multiplication: Certain bacteria have specialized structures (adherence factors, such as pili and capsule) which allow them to adhere to specific sites on surface of human cell, thereby enhancing their ability to cause diseases. The bacteria can be multiplied by growth at site of adherence and form colonization, or spread to another tissues by invasion. III. Invasion and damage of host tissues: The invasion is ability of microorganism to spread into host tissues, some bacteria are non-invasive, but multiply at site of adherence. Bacteria secrete several invasive factors that play role in pathogen a. Enzymes: 1. Collaginase and hyaluronidase ; which degrade collagen and hyaluronic acid ,thereby allowing the bacteria to spread through host tissues. 2. Coagulase; which accelerates the formation of fibrin clot from fibrinogen around bacteria to protect the bacteria from phagocytosis action. 3. IgA protease; which degrades this immunoglobulin; allowing the bacteria to adhere to mucous membrane. 4. leucocidin, which can destroy both neutrophile and macrophages. 4  5. Hemolysin which can destroy RBC 6. Capsule; prevent the phagocytes from adhering to bacteria therefore, capsule act as antiphagocytic factor. b. Toxin production: The bacteria produce two types of toxins, exotoxin and endotoxin. Exotoxin is produce by G+ ve and G- ve bacteria. In contrast, endotoxin which is produce by only G- ve bacteria. Exotoxin is protein, whereas endotoxin is lipid and carbohydrate. Exotoxin is good antigen and induce high titer of antibody (antitoxin), whereas endotoxin is weakly antigenic and not induce antitoxin. exotoxin is heat labile at 60C, when treated with formaldehyde (or acid or heat) can be converted in toxoid (the toxin is lost their toxicity but retain their antigenicity, therefore can be used as vaccine) whereas, endotoxin cannot be toxoid because the toxin is heat stable at more than 60C. 5 Table (4) the maine feature of exotoxin and endotoxin. 6