Microbiology Lab Practical-Sample Collection Guidelines (PDF)

Microbiology Laboratory Dr. Basma Samir (MD) “ ” 1 Types of Biological Specimens 2 SPUTUM WOUND URINE TISSUE FAECES BODY FLUIDS BLOOD BRONCHIAL / WASH / LAVAGE BONE MARROW T...

Microbiology Laboratory Dr. Basma Samir (MD) “ ” 1 Types of Biological Specimens 2 SPUTUM WOUND URINE TISSUE FAECES BODY FLUIDS BLOOD BRONCHIAL / WASH / LAVAGE BONE MARROW THORAT SWAB PUS EAR EYE 3 Collection Containers 4 Basic Concepts for Specimen Collection 5 The proper collection of a specimen for culture is the most important step in the recovery of pathogenic organisms responsible for infectious diseases. A poorly collected specimen may lead to failure in isolating the causative organism(s) and/or result in the recovery of contaminating organisms. General guidelines for specimen collection 6 Collect the specimen from the actual site of infection, avoiding contamination from adjacent tissues or secretions. Collect the specimen at optimal times (for example, early morning sputum for AFB culture). Collect a sufficient quantity of material. Use appropriate collection devices: sterile, leak-proof specimen containers. General guidelines for specimen collection 7 Use appropriate transport media. Check expiration date before inoculating collection device. Whenever possible, collect specimens prior to administration of antimicrobial agents Properly label the specimen and complete the test request form. The specific source of specimen is required. Example: wound, left leg. General guidelines for specimen collection 8 Minimize transport time. Maintain an appropriate environment between collection of specimens and delivery to the laboratory. If appropriate, decontaminate the skin surface. Use 70% alcohol (ALC) and 2% chlorhexidine or 1-2% tincture of iodine (TIO) to prepare the site. Allow a contact time of two minutes to maximize the antiseptic effect.  9 Thank You