Podcast
Questions and Answers
Match the following types of biological specimens with their corresponding types:
Match the following types of biological specimens with their corresponding types:
Sputum = Bronchial / Wash / Lavage Pus = Wound Urine = Body Fluids Blood = Throat Swab
Match the following general guidelines for specimen collection with their descriptions:
Match the following general guidelines for specimen collection with their descriptions:
Collect from the infection site = Avoid contamination from adjacent tissues Use appropriate transport media = Ensure specimen viability during transport Label the specimen correctly = Include specific source information Collect at optimal times = Enhance the chance of isolating pathogens
Match the following collection containers with their appropriate specimen types:
Match the following collection containers with their appropriate specimen types:
Sterile container = Urine Leak-proof container = Blood Culture swab = Pus Transport media = Sputum
Match the following antiseptic agents with their uses in skin preparation:
Match the following antiseptic agents with their uses in skin preparation:
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Match the following specimen collection concepts with their importance:
Match the following specimen collection concepts with their importance:
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Study Notes
Microbiology Laboratory
- Dr. Basma Samir (MD) is the instructor
Sample Collection in Microbiology
- Includes various types of biological specimens
Types of Biological Specimens
- Sputum
- Urine
- Feces
- Blood
- Bone marrow
- Pus
- Wound
- Tissue
- Body fluids
- Bronchial wash/lavage
- Throat swab
- Ear
- Eye
Generic Equipment Required
- Transport medium containing swab
- Wood sticks
- Sterile cup
- Sterile, disposable gloves
- Acid Citrate Dextrose (ACD) tube for blood collection
Collection Containers
- Variety of containers for different specimens
- Sterile, leak-proof containers are crucial
- Containers must be properly labeled with necessary patient information
Basic Concepts for Specimen Collection
- Proper specimen collection is critical for accurate diagnosis of infectious diseases
- Poor collection can lead to failure in isolating the causative organism(s) or contamination
General Guidelines for Specimen Collection
- Collect specimen from the site of infection, avoid contamination
- Collect specimens at optimal times (e.g., early morning sputum for AFB culture)
- Collect sufficient quantity of material
- Use sterile, leak-proof specimen containers
- Use appropriate transport media
- Check expiration date of collection devices
- Collect prior to antimicrobial administration whenever possible
- Properly label specimens and complete test request forms
- Specific specimen source (e.g., wound, left leg) must be noted
- Minimize transport time
- Maintain appropriate environment during transport
- Decontaminate skin surface if needed using 70% alcohol, 2% chlorhexidine, or 1-2% tincture of iodine (allow contact time of 2 minutes)
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Description
This quiz covers the essential techniques for collecting biological specimens in a microbiology laboratory. Learn about different types of specimens, required equipment, and the importance of proper specimen handling for accurate diagnosis of infectious diseases. Test your knowledge on guidelines and best practices in microbiology sample collection.
