Sequencing and Functional Genomics Part 1 PDF
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Adang
2025
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Summary
This document discusses modern genetic advancements, including recombinant cloning, DNA sequencing, and PCR. It explores the goals and reasons behind the Human Genome Project and highlights advancements in sequencing technologies, from Sanger sequencing to next-generation sequencing (Illumina, SMRT) and nanopore sequencing. The document touches on the applications of these advancements, such as the 1000 Genomes project and precision medicine.
Full Transcript
Modern - genetic advancements 1970s-1980s - Recombinant cloning - DNA seq. - PCR years -. Practice w/ 2 smaller genomes. Map DNA 3 , then sea 4. Used Sanger sea method.. First sea 5 of genome HPG goal...
Modern - genetic advancements 1970s-1980s - Recombinant cloning - DNA seq. - PCR years -. Practice w/ 2 smaller genomes. Map DNA 3 , then sea 4. Used Sanger sea method.. First sea 5 of genome HPG goals ident fl ↓ gather data for ethical vol Reasons - inventory - -genetic relationships - future research Reasons - - into abt human - archives -utilize for genetic # disease 1977 Sanger - Hourescent chopping , length, sequence 1. Cut 2. PCR 3899. - Read Gel chromosomes in genetics Physical mapping via dones hotgen eots sea by ovenape Zach Zardom/ com Sequencing needs good coverage yeast d C. elegans ↓ Drosophila challenges - Humans large genome read - Sanger few base pairs - Lots of coverage - repetitive DNA Transposable ↓ done-by-clone shotgen sequencing - Conclusion cheaper - faster - -high quality - $1 billion for first genome - ethical lessen concerns did Herose euchrom ativ ↓ heterochromatin finally sequenced HGD Covid vaccines 2nd gen Sanger lighted DNA ↓ Cheaper Illumina - short sea via strand M utresis ↓ 3rd gen causes companies to d 3rd - gen meaper/ faster for single molec. no cation amplifi SMRT - synthesizes single DNA molecule Presence portable P nanopore sea 2007 - 2015 SNP - single nucleotide differenc ↓ variation ↓ 2 / silent ↓ ↓ AA Wrong Stop 1000 genomes project - on focus differend precision medicine - individual treatment based on genetics
