#1 Lab 2 Microscope.pdf

Structure and Types of Microscopes Lab 02 Objective: To become familiar with the parts of the microscope and their functions. To learn how to operate and use the microscope. To Understand to handle and take care of a microscope. Why are Microscopes so important for studying Biology? The invention of...

Structure and Types of Microscopes Lab 02 Objective: To become familiar with the parts of the microscope and their functions. To learn how to operate and use the microscope. To Understand to handle and take care of a microscope. Why are Microscopes so important for studying Biology? The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms, study the structure of cells, and see the smallest parts of plants, animals, and fungi. Today, the microscope is still a commonly used tool to diagnosis illness in hospitals and clinics all over the world. Since their original invention, microscopes have moved beyond the simple visible light refracting lenses. Electrons, x-rays, and infrared rays are used by far more sophisticated (and expensive) microscopes to detect even smaller and smaller structures. Scanning electron microscopes are able to resolve viruses, which are far smaller than any cell. General Biology BIO 101 Structure and Types of Microscopes Lab 02 Handling the Microscope: Hold the microscope with one hand around the arm of the device, and the other hand under the base. This is the most secure way to hold and walk with the microscope. Avoid touching the lenses of the microscope. The oil and dirt on your fingers can scratch the glass. Clean smudges on the microscope glass with a piece of lens paper. Any other material, such as tissues or even cotton, can scratch the glass of the microscope. Rotate the nosepiece of the microscope all the way down to its lowest level when you have finished using the microscope. If you leave the nosepiece up while storing it, the gears on the device can wear out. If this happens, the nosepiece may not be able to lift and hold its position. Cover the microscope with the dust cover to protect it from dirt and other debris. Types: A light microscope (LM) is an instrument that uses visible light and magnifying lenses to examine small objects not visible to the naked eye. General Biology BIO 101 Structure and Types of Microscopes Lab 02 Magnification power- objective lens (4X, 10X, 40X) X power of eyepiece (10X) General Biology BIO 101 Structure and Types of Microscopes Lab 02 Electron Microscopies: There are two basic types of Electron Microscopies (EM) used to study subcellular structures: Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. An image is formed from the interaction of the electrons transmitted through the specimen; the image is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a charge-coupled device. General Biology BIO 101 Structure and Types of Microscopes Lab 02 A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the sample's surface topography and composition. General Biology BIO 101 Structure and Types of Microscopes Lab 02 General Biology BIO 101 Structure and Types of Microscopes Lab 02 General Biology BIO 101