Lab Activity 1 Basic Microscopy PDF

Laboratory Activity 1 Laboratory Worksheet 1: Basic Microscopy LEARNING OUTCOMES CLO 2. Define zoology as a branch of biology and recognize its significance in understanding the animal kingdom. LO1. Apply the different steps of scientific method in solving a problem. LO2. Familia...

Laboratory Activity 1 Laboratory Worksheet 1: Basic Microscopy LEARNING OUTCOMES CLO 2. Define zoology as a branch of biology and recognize its significance in understanding the animal kingdom. LO1. Apply the different steps of scientific method in solving a problem. LO2. Familiarize students with the scientific method and its application in zoological research. LO3. Manipulate the microscope and prepare specimens for study. --------------------------------------------------------------------------------------------------------------------------------------- PRE-LABORATORY QUESTIONS --------------------------------------------------------------------------------------------------------------------------------------- The following questions will help you in preparing for the laboratory activity by ensuring you understand key concepts and techniques related to this topic. (The pre-lab questions are not graded but must be answered or studied to prepare for the pre-lab discussion and laboratory activity) 1. A microscope has different lenses. What do you think is the purpose of each lens? 2. Why is it important to have clean materials during experiments? 3. What is the purpose of staining cheek cells during microscopic observation? --------------------------------------------------------------------------------------------------------------------------------------- MATERIALS --------------------------------------------------------------------------------------------------------------------------------------- Materials Qty Specs Item 1 piece Light microscope 1 piece Slide with cover slip 1 piece Magazine or any printed material 1 piece Toothpick 1 pad/roll Paper towels or tissue 5 ml Methylene blue 1 piece Dropper --------------------------------------------------------------------------------------------------------------------------------------- PROCEDURE --------------------------------------------------------------------------------------------------------------------------------------- A. Review of the Basic Microscopy 1. Cut a small letter “e” from a newspaper or printed material that is enough to fit a slide. 2. Place the letter “e” on top of the slide and add a drop of water to keep it in place. 3. Gently cover the glass slide with a coverslip. 4. Place the slide on the stage of the microscope and secure it using the stage clips. 5. Look through the ocular lens. Adjust the coarse and fine focus knobs until the letter comes into focus. Be careful while focusing so that the slide does not touch the lenses. 6. Record your observations B. Observing Human Cheek cells using a Microscope 1. Scrape the inner cheek with a clean toothpick to obtain thin sample of tissue. DO NOT GOUGE THE INSIDE OF YOUR CHEEKS! 2. Take a clean glass slide and place 1–2 drops of distilled water at its center. Carefully transfer the tissue to the slide and spread it evenly in the center. 3. Add a few drops of methylene blue. CAUTION: METHYLENE BLUE WILL STAIN CLOTHES AND SKIN. 4. Allow the stain to sit for 3–5 minutes to ensure proper staining of the cells. 5. Place the coverslip carefully and blot excess methylene by touching one side of the cover slip. 6. Place the specimen-containing slide on the stage. NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 1 7. Turn on the light source. 8. Adjust the coarse and fine adjustment knobs while looking through the eyepiece to focus the projected image and distance of the specimen. 9. Record your observations. Observe proper handling of the microscope. Handle microscope slides and coverslips carefully to avoid cuts from broken glass. Do not play with the heat and keep lighter away when not in use. SAFETY Adhere to all laboratory rules and guidelines. PRECAUTIONS All equipment and materials must be cleaned and dried properly. Working table must be cleaned. DISPOSAL AND Dispose waste materials properly according to your lab's disposal protocols. CLEAN-UP REFERENCES Staining Microscopic Specimen. https://openstax.org/books/microbiology/pages/2-4-staining-microscopic- specimens NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 1 LABORATORY REPORT Name: ______________________________________________________ Score: LAB ACTIVITY 1 _______________________________________________________________ _______________________________________________________________ Date Performed: ____________________ Group: ________ Date Submitted: _______________________ Results and Observations A. Review of Basic Microscopy Draw your observation of the letter “e” below. Write the function of each part. 1 2 3 9 6 8 4 7 5 Draw here the letter “e” PARTS FUNCTION 1) Ocular lens (eyepiece) 2) Revolving nosepiece 3) Objective lens 4) Coarse adjustment knob 5) Fine adjustment knob 6) Stage 7) Lamp 8) Condenser NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 1 9) Mechanical stage B. Microscopy of Cheek Cells Illustrate the cheek cell you have observed. Describe the specimen in the space provided. (5pts) Scanner LPO HPO Description: Description: Description: Post-Lab Questions Answer the following questions. Include relevant citations to support your explanation. No citation, no point. (Use the back portion of this paper) 1. When you place the letter “e” on the stage of the microscope, and looked through the ocular lens or eyepiece and all you see is black in the field view, what would you adjust to fix the problem? _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 1 2. How did switching between low, medium, and high magnifications change your view? _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ 3. Why do cheek cells have a flat, irregular shape? How does this aid their function? _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ 4. What might happen if the cells were not stained? _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ Summary and Conclusions In a minimum of 10 sentences, summarize the concepts you learned from this activity (5 pts) _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________. NS0400L – FUNDAMENTALS OF ZOOLOGY