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Questions and Answers
Which type of electron microscopy uses a beam of electrons transmitted through an ultra-thin specimen?
Which type of electron microscopy uses a beam of electrons transmitted through an ultra-thin specimen?
- Light microscope (LM)
- Transmission electron microscopy (TEM) (correct)
- Scanning electron microscope (SEM)
- Electron microscopy (EM)
What is the magnification power of the objective lens of 4X in a light microscope with an eyepiece power of 10X?
What is the magnification power of the objective lens of 4X in a light microscope with an eyepiece power of 10X?
- 4X
- 40X (correct)
- 10X
- 100X
What does a scanning electron microscope (SEM) produce images of?
What does a scanning electron microscope (SEM) produce images of?
- Visible light
- Ultra-thin specimens
- Subcellular structures
- Sample's surface topography and composition (correct)
What is the most secure way to hold and walk with a microscope?
What is the most secure way to hold and walk with a microscope?
What has the invention of the microscope allowed scientists to discover?
What has the invention of the microscope allowed scientists to discover?
What are some other types of microscopes besides the simple visible light refracting lenses?
What are some other types of microscopes besides the simple visible light refracting lenses?
What can scanning electron microscopes resolve that is smaller than any cell?
What can scanning electron microscopes resolve that is smaller than any cell?
What is the main use of microscopes in hospitals and clinics?
What is the main use of microscopes in hospitals and clinics?
Which type of microscope uses visible light and magnifying lenses to examine small objects not visible to the naked eye?
Which type of microscope uses visible light and magnifying lenses to examine small objects not visible to the naked eye?
Which type of electron microscopy uses a beam of electrons transmitted through an ultra-thin specimen?
Which type of electron microscopy uses a beam of electrons transmitted through an ultra-thin specimen?
What does a scanning electron microscope (SEM) produce images of?
What does a scanning electron microscope (SEM) produce images of?
What type of microscopy technique uses a fluorescent screen or photographic film to detect the image formed by transmitted electrons?
What type of microscopy technique uses a fluorescent screen or photographic film to detect the image formed by transmitted electrons?
What are the two basic types of Electron Microscopies used to study subcellular structures?
What are the two basic types of Electron Microscopies used to study subcellular structures?
What type of microscope produces images of a sample by scanning it with a focused beam of electrons?
What type of microscope produces images of a sample by scanning it with a focused beam of electrons?
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Study Notes
Electron Microscopy
- Transmission Electron Microscopy (TEM) uses a beam of electrons transmitted through an ultra-thin specimen for imaging.
- Scanning Electron Microscopy (SEM) produces images of the surface of a sample by scanning it with a focused beam of electrons.
- Two basic types of Electron Microscopies are TEM and SEM.
- Scanning Electron Microscopes can resolve structures smaller than any cell, often down to nanometer scales.
- TEM employs a fluorescent screen or photographic film to detect transmitted electrons' images.
Light Microscopy
- The effective magnification power of a light microscope with a 4X objective lens and a 10X eyepiece is 40X (4 x 10).
- Light microscopes use visible light and magnifying lenses to examine small objects that are not visible to the naked eye.
Handling Microscopes
- The most secure way to hold and walk with a microscope is to hold it with both hands: one hand on the arm and the other supporting the base.
Contributions of Microscopes
- The invention of the microscope has enabled scientists to discover microorganisms and cellular structures, vastly expanding knowledge in biology and medicine.
Other Microscopy Techniques
- Besides simple visible light microscopes, other types include fluorescence microscopes, phase-contrast microscopes, and confocal microscopes.
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