Organophosphate Poisoning and Rescue Experiment PDF

Intoxication and Detoxication of Organophosphates Experimental purposes  Master the symptom of intoxication induced by Organophosphates;  Observe the effect of atropine and pralidoxime methiodide (PAM) on detoxication of Organophosphates;  Compare the effect of atr...

Intoxication and Detoxication of Organophosphates Experimental purposes  Master the symptom of intoxication induced by Organophosphates;  Observe the effect of atropine and pralidoxime methiodide (PAM) on detoxication of Organophosphates;  Compare the effect of atropine and PAM Experimental Principle The mechanism and the symptom of intoxication induced by Organophosphates ？ The antidotes and their mechanism ？ The mechanism of intoxication of Organophosphates  symptoms of organophosphates intoxication Muscarinic effects  Eye - Blurred vision, miosis  Glands - Increased lacrimation  Cardiovascular - Bradycardia, hypotension  Respiratory –severe respiratory distress  Gastrointestinal - nausea and vomiting, abdominal pain, diarrhea, fecal incontinence  Genitourinary – Urine incontinence symptoms of organophosphates intoxication Nicotinic symptoms  N1: hypertension  N2: muscular tension decreased, Muscular twitching CNS effects  Anxiety  Seizures  Coma Antidotes and their mechanism Atropine Muscarinic antagonist  Relieves M symptoms, such as miosis, hypersalivation, fecal and Urine incontinence, inhibition of cardiovascular system.  PAM Cholinesterase reactivator  Experimental Material  Animal ： Rabbit ， 2-3 kg  Instrument: Rabbit-cage, syringes  Drugs 0.08 ％ dichlorovinyl dimethyl phosphate （ DDV ） 0.1 ％ atropine 2.5 ％ PAM Experimental Procedure  Weigh rabbit, observe and record their normal indexes, such as respiration, pupils, salivation, feces and urine, muscular tension, and muscular twitching.  Administration: 0.08 ％ DDV, 1ml/kg( intramuscular injection, im), and observe if the indexes changed. Experimental Procedure  When the intoxication symptoms become obvious(Seizures), inject atropine (1 ml/kg, ear intravenous injection ， iv ) to the rabbit.  Then, observe if the indexes have any changes, and record changes of each index.  After the M-symptoms disappear, inject PAM (2 ml/kg, iv) to the rabbit, then record changes of each index.  Results and analysis Normal After After After PAM Indexes status DDV atropine Pupils Saliva Feces/Urine Muscular Tension Muscular twitching  Questions Analyze the mechanism of Organophosphates intoxication ？ Analyze the detoxication mechanism of atropine and PAM ？