Lipoprotein Measurement Methods PDF

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WellBalancedRadiance8883

Uploaded by WellBalancedRadiance8883

Chattahoochee Technical College

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lipoprotein measurement electrophoresis lipid metabolism biology

Summary

This document provides an overview of lipoprotein measurement methods, including the use of electrophoresis. It also illustrates the different types of lipoproteins and their position on an electrophoresis gel.

Full Transcript

## Lipoprotein Measurement Methods - Measure physical properties: density, size, charge, apolipoprotein - Methods: ultracentrifugation, electrophoretic separation, chemical precipitation, chromatographic, immunochemical - Know the electrophoresis pattern shown below: ### Lipoprotein Electrophores...

## Lipoprotein Measurement Methods - Measure physical properties: density, size, charge, apolipoprotein - Methods: ultracentrifugation, electrophoretic separation, chemical precipitation, chromatographic, immunochemical - Know the electrophoresis pattern shown below: ### Lipoprotein Electrophoresis | Region | Lipoprotein | |---|---| | Origin | Chylomicron | | | LDL (B-Lipoprotein) | | | VLDL (Pre B-Lipoprotein) | | Mobility | HDL (a-Lipoprotein) | _The image shows a vertical electrophoresis gel with bands at different positions representing the different lipoproteins. The arrow indicates the direction of electrophoretic mobility, which is towards the anode (`+`)._