What is the main purpose of tissue fixation in histological techniques? Which fixative is commonly used in tissue preparation? What is the role of ethyl alcohol in tissue preparati... What is the main purpose of tissue fixation in histological techniques? Which fixative is commonly used in tissue preparation? What is the role of ethyl alcohol in tissue preparation? Why is clearing necessary before tissue embedding? What is the first step in the histological tissue preparation process? Which clearing agent is commonly used during tissue preparation? What is the primary reason for slicing tissue into thin sections? Which type of microtome is used for frozen tissue sections? What dye stains the nucleus blue in H&E staining? What cellular component does eosin stain? True or False Questions: Hematoxylin stains basic cellular components blue. The role of xylene is to replace alcohol during the clearing step. Embedding is performed before tissue dehydration. The rotary microtome is used for paraffin-embedded tissues. Staining is the last step in tissue preparation. Hematoxylin binds strongly to DNA and RNA due to their acidic properties. Clearing ensures that tissues are compatible with the embedding medium. Eosin is a basic dye used for staining nuclei. The nucleus appears dark blue or purple in H&E staining. PAS staining is used to detect carbohydrates in tissues.
Understand the Problem
The question set is focused on histological techniques and tissue preparation, including fixation, staining, and the materials used in these processes.
Answer
Fixation preserves tissue structure; formalin is a common fixative; ethyl alcohol for dehydration; clearing is for transparency; fixation is the first step; xylene is a clearing agent; thin sections for examination; cryostat for frozen sections; hematoxylin stains nuclei blue; eosin stains cytoplasm.
The main purpose of tissue fixation in histological techniques is to preserve the tissue's structure by preventing decay and autolysis. Formalin is the commonly used fixative. Ethyl alcohol aids in dehydration during tissue preparation. Clearing is necessary to make tissues transparent for embedding. The first step is fixation. Xylene is commonly used as a clearing agent. Slicing tissue into thin sections allows for detailed examination. For frozen sections, a cryostat microtome is used. Hematoxylin stains the nucleus blue, while eosin stains the cytoplasm. Hematoxylin stains basic components blue is True. Xylene replaces alcohol during clearing is True. Embedding before dehydration is False. Rotary microtome use for paraffin sections is True. Staining as the last step is False. Hematoxylin binding to acids is True. Clearing for embedding compatibility is True. Eosin as a basic dye is False. The nucleus appears blue/purple in H&E staining is True. PAS staining detects carbohydrates is True.
Answer for screen readers
The main purpose of tissue fixation in histological techniques is to preserve the tissue's structure by preventing decay and autolysis. Formalin is the commonly used fixative. Ethyl alcohol aids in dehydration during tissue preparation. Clearing is necessary to make tissues transparent for embedding. The first step is fixation. Xylene is commonly used as a clearing agent. Slicing tissue into thin sections allows for detailed examination. For frozen sections, a cryostat microtome is used. Hematoxylin stains the nucleus blue, while eosin stains the cytoplasm. Hematoxylin stains basic components blue is True. Xylene replaces alcohol during clearing is True. Embedding before dehydration is False. Rotary microtome use for paraffin sections is True. Staining as the last step is False. Hematoxylin binding to acids is True. Clearing for embedding compatibility is True. Eosin as a basic dye is False. The nucleus appears blue/purple in H&E staining is True. PAS staining detects carbohydrates is True.
More Information
Fixation is crucial for maintaining the integrity of tissue samples for microscopic analysis. With correct fixation, tissues remain structurally intact and can endure further processing without degradation.
Tips
A common mistake is confusing the sequential steps of tissue preparation, such as performing embedding before dehydration or mixing up clearing and staining processes. Remembering the correct sequence is key to avoiding mistakes.
Sources
- An Introduction to Specimen Processing - Leica Biosystems - leicabiosystems.com
- Tissue Fixation - an overview | ScienceDirect Topics - sciencedirect.com
- Histology, Staining - StatPearls - NCBI Bookshelf - ncbi.nlm.nih.gov
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