What characterizes the appearance of the cytoplasm in cells abundant with rough endoplasmic reticulum when stained with H&E?
Understand the Problem
The question is asking about the staining characteristics of cytoplasm in cells that have a lot of rough endoplasmic reticulum when stained with Hematoxylin and Eosin (H&E). It suggests that we need to identify how the cytoplasm appears based on the presence of ribosomes and the staining properties.
Answer
Blue or basophilic cytoplasm in H&E staining.
The cytoplasm in cells with abundant rough endoplasmic reticulum appears blue or basophilic when stained with H&E due to high rRNA content from ribosomes.
Answer for screen readers
The cytoplasm in cells with abundant rough endoplasmic reticulum appears blue or basophilic when stained with H&E due to high rRNA content from ribosomes.
More Information
Cells with abundant rough endoplasmic reticulum contain a high number of ribosomes, which are rich in RNA. This ribosomal RNA binds hematoxylin, a basophilic dye, resulting in a blue or purple appearance under H&E staining.
Tips
A common mistake is assuming that all cytoplasm appears pink in H&E staining. Remember, the presence of ribosomes in rough ER can cause a blue or basophilic appearance due to rRNA content.
Sources
- The Wonderful Colors of the Hematoxylin–Eosin Stain in Diagnostic... - journals.sagepub.com
- Connective Tissue - Michigan Histology - histology.medicine.umich.edu
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