In which of the following techniques do large proteins always come out of the column first? A) Cation-exchange chromatography B) Anion-exchange chromatography C) Affinity chromatog... In which of the following techniques do large proteins always come out of the column first? A) Cation-exchange chromatography B) Anion-exchange chromatography C) Affinity chromatography D) Gel filtration - according to size E) HPLC (High-performance liquid chromatography)
Understand the Problem
The question is asking about chromatography techniques and specifically which technique allows large proteins to exit the column first. This requires knowledge of biochemistry and chromatography methods.
Answer
Gel filtration - according to size.
The final answer is Gel filtration - according to size.
Answer for screen readers
The final answer is Gel filtration - according to size.
More Information
Gel filtration chromatography, also known as size exclusion chromatography, separates proteins based on their size. Large proteins elute first because they cannot enter the pores of the gel particles, unlike smaller proteins which take longer to pass through.
Tips
A common mistake is to confuse gel filtration with other forms of chromatography that separate based on charge or affinity.
Sources
- Gel-Filtration Chromatography - PMC - pmc.ncbi.nlm.nih.gov
- HPLC Column Choices for the Analysis of Proteins and Peptides - agilent.com
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