Year 2 Anatomy: Neural Crest, Eye and Ear
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Questions and Answers

What is the earliest sign of eye development seen in the embryo?

  • Closure of the neural tube
  • Pair of shallow grooves on each side of the forebrain (correct)
  • Formation of the lens vesicle
  • Development of the optic cup
  • From which sources is the optic cup and lens vesicle derived?

  • Neuroectoderm, surface ectoderm, mesoderm, and neural crest cells (correct)
  • Fibrous and vascular coats of the eye
  • Ectoderm of the body and mesoderm
  • Neural crest cells only
  • What happens during the invagination of the optic vesicles?

  • They induce changes in the surface ectoderm (correct)
  • They develop into the optic nerve
  • They become a single-walled structure
  • They migrate to form the fibrous coat of the eye
  • Which embryonic structure eventually develops into the retina?

    <p>Neuroectoderm (A)</p> Signup and view all the answers

    What occurs to the intraretinal space as development progresses?

    <p>It disappears as the walls of the optic cup oppose each other (D)</p> Signup and view all the answers

    What triggers the dissociation of neural crest cells from their neighboring cells?

    <p>Elevating and fusing of the neural folds (A)</p> Signup and view all the answers

    What is a primary consequence of defective neural crest cell migration in the digestive system?

    <p>Hirschsprung's disease (A)</p> Signup and view all the answers

    Which of the following structures is derived from neural crest cells?

    <p>Melanocytes (C)</p> Signup and view all the answers

    Which embryological abnormality can result from improper development of the eye?

    <p>Cataract formation (C)</p> Signup and view all the answers

    What role do neural crest cells play in the formation of cranial nerve ganglia?

    <p>They migrate and differentiate into ganglia. (C)</p> Signup and view all the answers

    Which condition is NOT associated with failure of neural crest cell migration?

    <p>Rett syndrome (D)</p> Signup and view all the answers

    What is the main result of the epithelial-to-mesenchymal transition of neural crest cells?

    <p>Enhanced ability to migrate and differentiate (B)</p> Signup and view all the answers

    The parasympathetic ganglia of the gastrointestinal tract are derived from which type of cells?

    <p>Neural crest cells (A)</p> Signup and view all the answers

    What structure does the hyaloid artery and vein eventually become after development?

    <p>Central artery and vein of the retina (C)</p> Signup and view all the answers

    During which week do the lips of the choroid fissure fuse?

    <p>7th week (A)</p> Signup and view all the answers

    Which part of the optic cup develops into the inner layer of the iris?

    <p>Pars iridica retinae (C)</p> Signup and view all the answers

    What is formed from the inner layer of mesenchyme surrounding the eye primordium?

    <p>Choroid (B)</p> Signup and view all the answers

    What is the role of the optic stalk during development?

    <p>It forms the optic nerve and chiasm (D)</p> Signup and view all the answers

    Which structure is responsible for the formation of the ciliary body and iris?

    <p>Optic cup (B)</p> Signup and view all the answers

    What happens to the lens vesicle during the 5th week of development?

    <p>It loses contact with the surface ectoderm (A)</p> Signup and view all the answers

    The substantia propria of the cornea originates from which layer?

    <p>Outer layer of mesenchyme (A)</p> Signup and view all the answers

    What is a characteristic of the optic nerve regarding myelination?

    <p>It becomes fully myelinated by 3 months after birth. (C)</p> Signup and view all the answers

    Which type of cells are responsible for myelinating the optic nerve?

    <p>Oligodendrocytes (D)</p> Signup and view all the answers

    Which condition results from the failure of closure of the choroid fissure?

    <p>Coloboma iridis (D)</p> Signup and view all the answers

    Which structure is derived from the first pharyngeal cleft?

    <p>Tympanic membrane (B)</p> Signup and view all the answers

    What do the malleus and incus originate from?

    <p>1st pharyngeal arch (A)</p> Signup and view all the answers

    What forms the connective tissue surrounding the inner ear structures?

    <p>Neural crest (B)</p> Signup and view all the answers

    Which component is responsible for maintaining balance in the inner ear?

    <p>Saccule (A)</p> Signup and view all the answers

    Which statement is true about the optic nerve post-transection?

    <p>It cannot regenerate. (B)</p> Signup and view all the answers

    What is the origin of the definitive eardrum?

    <p>Two germ layers (A)</p> Signup and view all the answers

    Which structures develop from the first and second pharyngeal arches?

    <p>Auricular hillocks (A)</p> Signup and view all the answers

    Which of the following is NOT a congenital malformation of the ear?

    <p>Tympanic membrane rupture (A)</p> Signup and view all the answers

    What is the role of neural crest cells in the pharyngeal arches?

    <p>Contribution to skeletal components of the face (C)</p> Signup and view all the answers

    Which pharyngeal cleft contributes to the formation of the external auditory meatus?

    <p>First pharyngeal cleft (B)</p> Signup and view all the answers

    What comprises each pharyngeal arch?

    <p>Mesenchymal tissue covered by ectoderm and endoderm (B)</p> Signup and view all the answers

    Which of the following is a derivative of the first pharyngeal arch?

    <p>Incus (A)</p> Signup and view all the answers

    Which statement about auricular anomalies is true?

    <p>Microtia involves an underdeveloped external ear. (C)</p> Signup and view all the answers

    What developmental structure does the neural crest primarily differentiate into during eye formation?

    <p>Fibrous and vascular coats of the eye (D)</p> Signup and view all the answers

    Which embryonic structure forms the lens of the eye during development?

    <p>Surface ectoderm (B)</p> Signup and view all the answers

    During eye development, what is the significance of the optic vesicles?

    <p>They form the double-walled optic cup. (B)</p> Signup and view all the answers

    Which of the following statements best describes the fate of the intraretinal space during eye development?

    <p>It completely disappears as the optic cup develops. (B)</p> Signup and view all the answers

    What is the primary source of the retina and optic nerve in developing eyes?

    <p>Neuroectoderm (C)</p> Signup and view all the answers

    What is the primary consequence of neural crest cells failing to migrate into the bowel walls in Hirschsprung's disease?

    <p>Defective parasympathetic plexus (B)</p> Signup and view all the answers

    Which of the following structures is NOT a derivative of neural crest cells?

    <p>Liver parenchyma (C)</p> Signup and view all the answers

    What abnormality can occur as a result of improper development of the eye due to issues with neural crest cells?

    <p>Cataract formation (B)</p> Signup and view all the answers

    Congenital deafness can be attributed to issues during the development of which structure?

    <p>Inner ear (D)</p> Signup and view all the answers

    Which condition is primarily characterized by delayed passage of meconium and involves issues with neural crest cells?

    <p>Hirschsprung's disease (B)</p> Signup and view all the answers

    Failure of which neural crest derivative can lead to congenital megacolon?

    <p>Parasympathetic ganglia of the gastrointestinal tract (A)</p> Signup and view all the answers

    Which embryological abnormality specifically involves the formation of an incomplete optic fissure?

    <p>Coloboma (C)</p> Signup and view all the answers

    What structure forms the iridopupillary membrane during development?

    <p>Inner layer of mesenchyme (D)</p> Signup and view all the answers

    Which part of the developing optic cup gives rise to the ciliary body?

    <p>Pars ciliaris retinae (D)</p> Signup and view all the answers

    During which week does the choroid fissure close?

    <p>7th week (D)</p> Signup and view all the answers

    Which structures are formed from the mesoderm present between the optic cup and surface epithelium?

    <p>Ciliary muscles and sphincter pupillae (C)</p> Signup and view all the answers

    What does the outer layer of the mesenchyme surrounding the eye primordium differentiate into?

    <p>Sclera (D)</p> Signup and view all the answers

    What developmental structure is primarily involved in forming the optic nerve?

    <p>Optic stalk (D)</p> Signup and view all the answers

    Which layer of the optic cup is responsible for forming the pigment layer of the retina?

    <p>Outer wall of the optic cup (B)</p> Signup and view all the answers

    The anterior chamber of the eye develops through which process?

    <p>Vacuolisation (B)</p> Signup and view all the answers

    What is a characteristic of the optic nerve regarding its ability to regenerate?

    <p>The optic nerve does not regenerate after transection. (B)</p> Signup and view all the answers

    Which structure forms from the neural crest surrounding the inner ear?

    <p>Bony labyrinth (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the components of the external ear?

    <p>It includes the tympanic membrane and auricle. (B)</p> Signup and view all the answers

    What type of cells are responsible for myelination of the optic nerve?

    <p>Oligodendrocytes (A)</p> Signup and view all the answers

    Which condition is indicated by the absence of the lens?

    <p>Congenital Aphakia (D)</p> Signup and view all the answers

    How is the tympanic membrane primarily formed?

    <p>From first pharyngeal membrane components. (C)</p> Signup and view all the answers

    Which of the following statements is true regarding the development of the cochlear duct?

    <p>It is an element of the otic vesicle's ventral region. (D)</p> Signup and view all the answers

    What are the derivatives of the first and second pharyngeal arches related to the ear?

    <p>They develop into the malleus, incus, and stapes. (D)</p> Signup and view all the answers

    What is the primary embryonic origin of the definitive eardrum?

    <p>Two germ layers (A)</p> Signup and view all the answers

    Which of the following auricular anomalies is characterized by a complete absence of the external ear?

    <p>Anotia (C)</p> Signup and view all the answers

    Which pharyngeal cleft is known to contribute to the formation of the external auditory meatus?

    <p>First cleft (C)</p> Signup and view all the answers

    What type of cells migrate into the pharyngeal arches to contribute to the skeletal components of the face?

    <p>Neural crest cells (C)</p> Signup and view all the answers

    What structures primarily originate from the mesenchymal tissue of the pharyngeal arches?

    <p>Skeletal muscles of the face (D)</p> Signup and view all the answers

    Which condition results from congenital malformation associated with the first and second pharyngeal arches?

    <p>Microtia (A)</p> Signup and view all the answers

    Which of the following is a main component of each pharyngeal arch?

    <p>Core of mesenchymal tissue (C)</p> Signup and view all the answers

    What is the fate of the epithelial lining of the first pharyngeal cleft?

    <p>It forms the tympanic membrane. (D)</p> Signup and view all the answers

    Flashcards

    Neural Crest Cells Origin

    Specialized cells found at the border of the developing neural tube

    Neural Crest Cell Migration

    Neural crest cells leave the neuroectoderm and travel to different parts of the developing embryo to make different tissues

    Hirschsprung's Disease Cause

    Failure of neural crest cells to migrate properly to the bowel wall

    Hirschsprung's Disease Effect

    A congenital disorder resulting in a lack of certain nerve cells (aganglionic segment) in the bowel, commonly the rectum and sigmoid colon

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    Neural Crest Derivatives (face)

    Neural crest cells differentiate into connective tissues and bones of the face and skull

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    Neural Crest Derivatives (autonomic nervous system)

    Contribute to ganglia of the sympathetic and parasympathetic nervous systems

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    Coloboma/Cataract Development

    Eye development abnormalities that affect the structure and function

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    Congenital Deafness Cause

    Problems during ear development are possible factors to the condition

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    Eye development sources

    The eye develops from neuroectoderm, surface ectoderm, mesoderm, and neural crest cells.

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    Earliest sign of eye development

    Shallow grooves on each side of the forebrain are the initial indicators of eye formation in a 22-day embryo.

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    Optic Vesicles formation

    Initially shallow grooves, the development leads to outpocketings called optic vesicles, as the neural tube closes.

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    Optic cup formation

    The optic vesicles then invaginate further and forms a double-walled structure, like a cup; the optic cup

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    Lens vesicle role

    The lens vesicle is a structure which is formed when the optic vesicle comes into contact with the surface ectoderm that induces changes and forms a crucial part in eye development.

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    Choroid fissure

    A fissure that allows passage of hyaloid vessels during development.

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    Hyaloid artery/vein

    Temporary vessels that become central retinal vessels.

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    Lens placode

    Surface ectoderm cells involved in lens development.

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    Lens vesicle

    Invaginated lens placode.

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    Choroid

    Highly vascularized pigmented layer behind the retina, part of the eye.

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    Sclera

    Outer protective layer of the eye wall.

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    Anterior chamber

    Space in front of the iris and behind cornea, part of the eye's structure.

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    Optic nerve (CN II)

    Nerve formed by axons from ganglion cells of retina, part of the eye.

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    Optic nerve myelination

    The optic nerve (CN II) is not fully myelinated until 3 months after birth. Myelination is done by Oligodendrocytes.

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    Optic nerve regeneration

    The optic nerve (CN II) cannot regenerate after being severed or damaged.

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    Coloboma iridis

    A birth defect where the iris doesn't fully form due to an incomplete closure of the choroid fissure.

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    Aniridia

    A condition where the iris is completely absent at birth.

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    Ear development origins

    The ear develops from a thickened ectodermal placode at the rhombencephalon and from the first and second pharyngeal arches.

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    Ear parts

    The ear has three main sections: the outer ear (auricle, external auditory meatus, eardrum), the middle ear (malleus, incus, stapes and the auditory tube), and the inner ear (cochlear duct, semicircular canals).

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    Membranous labyrinth

    The membranous labyrinth includes the cochlear duct, utricle, saccule, and semicircular canals inside the bony labyrinth.

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    Tympanic membrane derivation

    The eardrum (tympanic membrane) develops from the first pharyngeal membrane, combining ectoderm of the first pharyngeal cleft and endoderm of the first pharyngeal pouch.

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    Eardrum Development

    The eardrum, or tympanic membrane, is a three-layered structure formed from two germ layers: ectoderm and endoderm. The outer layer originates from ectoderm, while the inner layer comes from endoderm.

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    Auricle Development

    The outer ear, or auricle, develops from six mesenchymal proliferations (auricular hillocks) surrounding the first pharyngeal cleft.

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    Congenital Deafness Types

    Congenital deafness can occur in the inner ear (due to factors like rubella), middle ear (due to problems with the first and second pharyngeal arches), or external ear (due to agenesis).

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    Auricular Anomalies

    These are birth defects of the external ear, including accessory tragus (extra cartilage), microtia (small ear), anotia (absence of ear), and preauricular pits or sinuses (depressions or openings near the ear).

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    Pharyngeal Arch Composition

    Each pharyngeal arch consists of a core of mesenchymal tissue covered by surface ectoderm (outer layer) and endoderm (inner layer) epithelium. Neural crest cells migrate into the arches, contributing to facial skeletal components, while mesoderm cells form face and neck muscles.

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    First Pharyngeal Arch Derivatives

    The first pharyngeal arch has a dorsal portion (maxillary process) and a ventral portion (mandibular process) containing Meckel's cartilage, which contributes to the development of the lower jaw.

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    Pharyngeal Pouch Function

    Pharyngeal pouches are outpouchings of the primitive pharynx that contribute to the development of important structures like the parathyroid glands, thymus, and tonsils.

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    First Pharyngeal Cleft Function

    The first pharyngeal cleft, the only one to contribute to the development of the external auditory meatus, is involved in the formation of the ear canal. This cleft also contributes to the formation of the tympanic membrane.

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    Neural Crest Cells

    A special group of cells found at the border of the developing neural tube, which migrate to various locations and differentiate into diverse structures.

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    Hirschsprung's Disease

    A birth defect where neural crest cells fail to migrate to the bowel wall, resulting in a lack of nerve cells necessary for proper digestion and elimination.

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    Eye Development

    The eye develops from several sources: neuroectoderm, surface ectoderm, mesoderm, and neural crest cells. These cells interact to form the different parts of the eye.

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    Coloboma

    A birth defect that occurs when the choroid fissure, a gap involved in eye development, fails to close completely.

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    Ear Development

    The ear develops from specialized ectoderm (placode) and contributes from pharyngeal arches, specifically the first and second, which contribute to its different parts.

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    Congenital Deafness

    Hearing loss present at birth due to problems during ear development, including issues with the inner, middle, or external parts.

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    Pharyngeal Arches

    Curved structures in the developing embryo that give rise to important facial structures, muscles, and nerves. Each arch involves a core of mesenchymal tissue, covered by ectoderm and endoderm.

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    What are the four sources of eye development?

    The eye develops from four embryonic sources: neuroectoderm (retina, posterior iris, optic nerve), surface ectoderm (lens, cornea epithelium), mesoderm (vascular and fibrous layers), and neural crest cells (choroid, sclera, corneal endothelium).

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    What is the earliest sign of eye development?

    The first sign of eye development is seen in a 22-day embryo as two shallow grooves on either side of the forebrain.

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    What is the function of the lens vesicle?

    The lens vesicle, formed from the surface ectoderm, interacts with the optic vesicles and induces changes leading to the formation of the double-walled optic cup. This cup is a crucial structure for the eye's development.

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    What is the optic cup?

    The optic cup is a double-walled structure formed by the invagination of the optic vesicles. It eventually becomes the retina and the pigmented epithelial layer of the eye.

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    What is the difference between the inner and outer walls of the optic cup?

    The inner wall of the optic cup develops into the neural retina (light-sensitive layer), while the outer wall forms the pigmented epithelium, which supports the retina.

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    Hyaloid vessels

    Temporary blood vessels that supply the developing lens and retina.

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    Central artery and vein of the retina

    The main blood vessels that supply the retina after the hyaloid vessels disappear.

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    What is the role of pharyngeal pouches?

    Pharyngeal pouches are outpouchings of the primitive pharynx that contribute to the development of important structures like the parathyroid glands, thymus, and tonsils.

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    Function of the First Pharyngeal Cleft

    The first pharyngeal cleft, the only one to contribute to the development of the external auditory meatus, is involved in the formation of the ear canal. This cleft also contributes to the formation of the tympanic membrane.

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    Study Notes

    Neural Crest, Eye and Ear

    • The class is Year 2, Semester 1
    • The lecturer is Dr. Vijayal Akshmi S B, from the Department of Anatomy
    • The date of the lecture is 26-11-2024

    Learning Outcomes

    • Describe the origin and migration of neural crest cells
    • Explain how failure of normal neural crest migration can cause pathologies, like Hirschsprung's disease
    • Describe the development of the eye, and explain how embryological abnormalities like coloboma or cataract formation occur
    • Describe the development of the ear, and explain how problems like congenital deafness occur
    • Describe the pharyngeal arches and their derivatives

    Neural Crest Cells

    • Neural crest cells are a specialized cell population situated at the lateral border/crest of the neuroectoderm
    • These cells detach from their neighbours as neural folds elevate and fuse to form the neural tube
    • Neural crest cells undergo an epithelial-to-mesenchymal transition
    • They migrate extensively to various locations in the body, contributing to diverse structures

    Neural Crest Derivatives

    • Connective tissue and bones of the face and skull
    • Cranial nerve ganglia
    • C cells of the thyroid gland
    • Conotruncal septum in the heart
    • Odontoblasts
    • Dermis in face and neck
    • Spinal (dorsal root) ganglia
    • Sympathetic chain and pre-aortic ganglia
    • Parasympathetic ganglia of the gastrointestinal tract
    • Adrenal medulla
    • Schwan cells
    • Glial cells
    • Meninges (forebrain)
    • Melanocytes
    • Smooth muscle cells to blood vessels of the face and forebrain

    Hirschsprung's Disease

    • Failure of neural crest cells to migrate into the bowel wall
    • Defective parasympathetic plexus (Auerbach's and Meissner's)
    • Aganglionic segment, particularly the sigmoid and rectum
    • Delayed passage of meconium
    • Constipation, vomiting
    • Abdominal distension

    Development of Eye

    • The earliest sign of eye development is seen in the 22-day embryo as shallow grooves on each side of the forebrain
    • Closure of the neural tube forms outpocketings called optic vesicles
    • Four sources: neuroectoderm of the forebrain, surface ectoderm of the head, mesoderm between these two tissues, and neural crest cells.
    • Neuroectoderm gives rise to the retina, posterior layers of iris and optic nerve
    • Surface ectoderm creates the lens of the eye and the corneal epithelium
    • Mesoderm between neuroectoderm and surface ectoderm forms the fibrous and vascular coats(choroid, sclera) of the eye
    • Neural crest cells migrate, contributing to the choroid, sclera, and corneal endothelium
    • Optic vesicles form the optic cup, the lens placode invaginates to create the lens vesicle.
    • Choroid fissure allows the hyaloid vessels during development, the lumen then disappears.

    Optic Cup and Lens Vesicle

    • Lens vesicle contacts the surface ectoderm, inducing changes
    • Optic vesicles invaginate, forming the double-walled optic cup
    • Inner and outer walls of the cup are initially separated by an intraretinal space; this space disappears during development as the walls come together
    • Surface ectoderm contacting the optic vesicle forms lens placodes, which invaginate into lens vesicles.

    Development of Ear

    • The ear (inner, middle, external) develops from the thickened ectodermal placode located at the rhombencephalon (hindbrain) and first and second pharyngeal arches.
    • The otic placode induces vesicles, the dorsal vestibular region and ventral cochlear region.
    • The otic vesicles create the cochlear duct and semicircular canals and the endolymphatic duct.
    • Neural crest forms surrounding connective tissue (mesenchyme) which becomes cartilaginous and then ossifies
    • The first arch is responsible for the malleus and incus, and the second arch is responsible for the stapes

    Pharyngeal Arches and Derivatives

    • Develop during the 4th and 5th weeks
    • Consist of arch (mesoderm), groove/clefts (ectoderm), and pouches (endoderm)
    • Arches contribute to facial muscles, bones (malleus, incus, stapes), and the hyoid bone and laryngeal cartilages

    Fate of Pharyngeal Arch

    • Maxillary prominence (smaller) : maxilla, zygomatic, and temporal bones
    • Mandibular prominence (larger): lower face structures

    Derivatives of Pharyngeal Arches

    • First arch cartilages (Meckel's cartilage): middle ear ossicles (malleus, incus), sphenomandibular ligament, mandible
    • Second arch cartilages (Reichert's cartilage): stapes, stylopharyngeus, stylohyoid ligament, lesser and greater horn of the hyoid bone
    • Third arch cartilage: inferior part of hyoid bone, greater horn of hyoid bone
    • Fourth and sixth arches cartilages: laryngeal cartilages (cricoid, thyroid, etc.)

    Pharyngeal Pouches

    • Endodermal outpouchings from the pharynx
    • First pouch: auditory tube, tympanic cavity, mastoid antrum
    • Second pouch: palatine tonsils, tonsillar crypts, superior parathyroid gland
    • Third pouch: inferior parathyroid glands, thymus
    • Fourth pouch: superior parathyroid glands
    • Fifth pouch: ultimobranchial body, parafollicular cells of thyroid

    Pharyngeal Clefts

    • Ectodermal grooves on the outside of the pharynx
    • First cleft: external auditory meatus
    • Other clefts contribute to the cervical sinus, which normally disappears.

    Clinical Correlates

    • Coloboma iridis: due to failure of closure of choroid fissure
    • Persistence of iridopupillary membrane: causes a lack of iris tissues
    • Aniridia: absence of iris tissue
    • Congenital Aphakia: absence of the lens
    • Congenital Cataracts: opaque lens
    • Synophthalmia/Cyclopia: fused or single eye

    1st Arch Defects

    • Mandibulofacial dysostosis: autosomal dominant genetic disorder
    • Micrognathia: underdevelopment of the mandible
    • Cleft palate
    • Glossoptosis: tongue posteriorly placed
    • Robin sequence: a syndrome characterized by several developmental issues
    • Treacher Collins syndrome

    3rd & 4th Pouch Syndrome

    • Absence of thymus
    • Absence of parathyroid glands
    • Persistent truncus arteriosus
    • Abnormal external ear
    • Micrognathia - underdevelopment of the mandible (some cases)

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    Description

    This quiz focuses on the neural crest, its development, and related abnormalities in eye and ear development. You will learn about the migration of neural crest cells, associated pathologies, and the formation of the eye and ear structures. Delve into key topics such as Hirschsprung's disease and congenital deafness.

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